Sorting out the complexity of SR protein functions

RNA

Volumn 6, Issue 9, 2000, Pages 1197-1211

  Graveley, Brenton R ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL PROTEIN; PROTEIN SR; UNCLASSIFIED DRUG;

HUMAN; MOLECULAR RECOGNITION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ASSEMBLY; PROTEIN FAMILY; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; REVIEW; RNA SPLICING; SPLICEOSOME;

EXONS; HUMANS; NUCLEAR PROTEINS; PHOSPHOPROTEINS; PROTEIN CONFORMATION; RIBONUCLEOPROTEINS, SMALL NUCLEAR; RNA PRECURSORS; RNA SPLICING; RNA-BINDING PROTEINS; SPLICEOSOMES;

EID: 0033835333     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838200000960     Document Type: Review

References (142)

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4. Bimolecular exon ligation by the human spliceosome
5. A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs
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7. Molecular cloning of htra2-beta-1 and htra2-beta-2, two human homologs of tra-2 generated by alternative splicing
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9. Exon recognition in vertebrate splicing
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11. The SRm160/300 splicing coactivator subunits
12. SR-related proteins and the processing of messenger RNA precursors
13. A coactivator of pre-mRNA splicing
14. Regulation of sexual differentiation in D. melanogaster via alternative splicing of RNA from the transformer gene
15. Role of the constitutive splicing factors U2AF65 and SAP49 in suboptimal RNA splicing of novel retroviral mutants
16. Identification of a bidirectional splicing enhancer: Differential involvement of SR proteins in 5' and 3' splice site activation
17. Enhancer-dependent interaction between 5' and 3' splice sites in trans
18. Splicing of precursors to mRNAs by the spliceosomes
19. Drosophila doublesex gene controls somatic sexual differentiation by producing alternatively spliced mRNAs encoding related sex-specific polypeptides
20. Functional analysis of pre-mRNA splicing factor SF2/ASF structural domains
21. A specific subset of SR proteins shuttles continuously between the nucleus and the cytoplasm
22. Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors
23. The splicing factors 9G8 and SRp20 transactivate splicing through different and specific enhancers
24. Characterization and cloning of the human splicing factor 9G8: A novel 35 kDa factor of the serine/arginine protein family
25. Characterization of a Drosophila protein associated with boundaries of transcriptionally active chromatin
26. RNA splicing specificity determined by the coordinated action of RNA recognition motifs in SR proteins
27. Evidence for the function of an exonic splicing enhancer after the first catalytic step of pre-mRNA splicing
28. A two-step mechanism for 5' and 3' splice-site pairing
29. The Clk/Sty protein kinase phosphorylates SR splicing factors and regulates their intranuclear distribution
30. An element in the 5' common exon of the NCAM alternative splicing unit interacts with SR proteins and modulates 5' splice site selection
31. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP
32. A U6 snRNA: Pre-mRNA interaction can be rate-limiting for U1-independent splicing
33. A human homologue of the Drosophila sex determination factor transformer-2 has conserved splicing regulatory functions
34. Analysis of the functional specificity of RS domains in vivo
35. Conservation of regulated alternative splicing and identification of functional domains in vertebrate homologs to the Drosophila splicing regulator, suppressor-of-white-apricot
36. Novel delayed-early and highly insulin-induced growth response genes. Identification of HRS, a potential regulator of alternative pre-mRNA splicing
37. Protein phosphorylation plays an essential role in the regulation of alternative splicing and sex determination in Drosophila
38. The SRm160/300 splicing coactivator is required for exon-enhancer function
39. The [U4/U6.U5] tri-snRNP-specific 27K protein is a novel SR protein that can be phosphorylated by the snRNP-associated protein kinase
40. Luc7p, a novel yeast U1 snRNP protein with a role in 5' splice site selection
41. Specific commitment of different pre-mRNAs to splicing by single SR proteins
42. The superfamily of arginine/serine-rich splicing factors
43. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus
44. The 35-kDa mammalian splicing factor SC35 mediates specific interactions between U1 and U2 small nuclear ribonucleoprotein particles at the 3' splice site
45. Isolation of a complementary DNA that encodes the mammalian splicing factor SC35
46. General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5' and 3' splice site selection
47. A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro
48. Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators
49. The plant U1 small nuclear ribonucleo-protein particle 70K protein interacts with two novel serine/arginine-rich proteins
50. An SC35-like protein and a novel serine/arginine-rich protein interact with Arabidopsis U1-70K protein
51. A systematic analysis of the factors that determine the strength of pre-mRNA splicing enhancers
52. Arginine/serine-rich domains of SR proteins can function as activators of pre-mRNA splicing
53. Identification and characterization of srp1, a gene of fission yeast encoding a RNA binding domain and a RS domain typical of SR splicing factors
54. A serine kinase regulates intracellular localization of splicing factors in the cell cycle
55. Purification and characterization of a kinase specific for the serine- and arginine-rich pre-mRNA splicing factors
56. Characterization by cDNA cloning of two new human protein kinases. Evidence by sequence comparison of a new family of mammalian protein kinases
57. Common themes in the function of transcription and splicing enhancers
58. Serine-arginine (SR)-rich splicing factors have an exon-independent function in pre-mRNA splicing
59. U1 snRNP-ASF/SF2 interaction and 5' splice site recognition: Characterization of required elements
60. Molecular cloning of a novel human cdc2/CDC28-like protein kinase
61. Pre-mRNA Splicing of IgM exons M1 and M2 is directed by a juxtaposed splicing enhancer and inhibitor
62. Regulation of adenovirus alternative RNA splicing by dephosphorylation of SR proteins
63. Protein-protein interactions and 5' splice site recognition in mammalian mRNA precursors
64. The essential pre-mRNA splicing factor SF2 influences 5' splice site selection by activating proximal sites
65. Purification and characterization of pre-mRNA splicing factor SF2 from HeLa cells
66. Functional expression of cloned human splicing factor SF2: Homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators
67. Antagonism between RSF1 and SR proteins for both splice-site recognition in vitro and Drosophila development
68. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding
69. Identification of a plant serine-arginine-rich protein similar to the mammalian splicing factor SF2/ASF
70. Arginine/serine-rich domains of the su(wa) and tra RNA processing regulators target proteins to a subnuclear compartment implicated in splicing
71. Distinct factor requirements for exonic splicing enhancer function and binding of U2AF to the polypyrimidine tract
72. Identification of functional exonic splicing enhancer motifs recognized by individual SR proteins
73. Exonic splicing enhancer motif recognized by human SC35 under splicing conditions
74. Functional characterization of SR and SR-related genes in Caenorhabditis elegans
75. A novel family of plant splicing factors with a Zn knuckle motif: Examination of RNA binding and splicing activities
76. Pre-mRNA splicing in plants: Characterization of Ser/Arg splicing factors
77. Characterization of a novel arginine/serine-rich splicing factor in Arabidopsis
78. An exonic enhancer is required for inclusion of an essential exon in the SMA-determining gene SMN
79. Srp2, an SR protein family member of fission yeast: In vivo characterization of its modular domains
80. Assembly of specific SR protein complexes on distinct regulatory elements of the Drosophila double-sex splicing enhancer
81. Substrate specificities of SR proteins in constitutive splicing are determined by their RNA recognition motifs and composite pre-mRNA exonic elements
82. Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing
83. Inhibition of msl-2 splicing by Sex-lethal reveals interaction between U2AF35 and the 3' splice site AG
84. An ATP-independent complex commits pre-mRNA to the mammalian spliceosome assembly pathway
85. Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo
86. The dynamics of a pre-mRNA splicing factor in living cells
87. The cellular organization of gene expression
88. RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo
89. CROP/Luc7A, a novel serine/arginine-rich protein, isolated from cisplatin-resistant cell line
90. Identification of a putative RNA helicase (HRH1), a human homolog of yeast Prp22
91. The protein kinase Clk/Sty directly modulates SR protein activity: Both hyper- and hypophosphorylation inhibit splicing
92. A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein
93. Initial splice-site recognition and pairing during pre-mRNA splicing
94. Spliceosome assembly
95. The SR protein B52/SRp55 is essential for Drosophila development
96. SR proteins escort the U4/U6·U5 tri-snRNP to the spliceosome
97. Specific phosphorylation of SR proteins by mammalian DNA topoisomerase I
98. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription
99. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly
100. Control of male sexual behavior and sexual orientation in Drosophila by the fruitless gene
101. Developmental regulation of SR protein phosphorylation and activity
102. Multiple distinct splicing enhancers in the protein-coding sequences of a constitutively spliced pre-mRNA
103. Selection and characterization of pre-mRNA splicing enhancers: Identification of novel SR protein- specific enhancer sequences
104. Identification and characterization of three members of the human SR family of pre-mRNA splicing factors
105. Exonic splicing enhancers contribute to the use of both 3' and 5' splice site usage of rat betatropomyosin pre-mRNA
106. A specific RNA hairpin loop structure binds the RNA recognition motifs of the Drosophila SR protein B52
107. Scanning from an independently specified branch point defines the 3' splice site of mammalian introns
108. Characterization of SRp46, a novel human SR splicing factor encoded by a PR264/SC35 retropseudogene
109. SWAP pre-mRNA splicing regulators are a novel, ancient protein family sharing a highly conserved sequence motif with the prp21 family of constitutive splicing proteins
110. A putative mammalian RNA helicase with an arginine-serine-rich domain colocalizes with a splicing factor
111. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer
112. Sequence-specific RNA binding by an SR protein requires RS domain phosphoryiation: Creation of an SRp40-specific splicing enhancer
113. The human splicing factors ASF/SF2 and SC35 possess distinct, functionally significant RNA binding specificities
114. Determinants of SR protein specificity
115. Human Tra2 proteins are sequence-specific activators of pre-mRNA splicing
116. SR proteins can compensate for the loss of U1 snRNP functions in vitro
117. Modulation of 5' splice site choice in pre-messenger RNA by two distinct steps
118. The human U5 snRNP-specific 100-kD protein is an RS domain-containing, putative RNA helicase with significant homology to the yeast splicing factor Prp28p
119. Positive control of pre-mRNA splicing in vitro
120. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA
121. A splicing enhancer exhibits both constitutive and regulated activities
122. SRPK2: A differentially expressed SR protein-specific kinase involved in mediating the interaction and localization of pre-mRNA splicing factors in mammalian cells
123. Overexpression of the SR proteins ASF/SF2 and SC35 influences alternative splicing in vivo in diverse ways
124. Targeted disruption of an essential vertebrate gene: ASF/SF2 is required for cell viability
125. Genetic analysis of the SR protein ASF/SF2: Interchangeability of RS domains and negative control of splicing
126. Intrinsic U2AF binding is modulated by exon enhancer signals in parallel with changes in splicing activity
127. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing
128. Functional recognition of the 3' splice site AG by the splicing factor U2AF35
129. Phosphorylation of the ASF/SF2 RS domain affects both protein-protein interactions and is necessary for splicing
130. Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2
131. SR proteins: A conserved family of pre-mRNA splicing factors
132. Distinct functions of SR proteins in alternative pre-mRNA splicing
133. Human SR proteins and isolation of a cDNA encoding SRp75
134. Identification, purification, and biochemical characterization of U2 small nuclear ribonucleoprotein auxiliary factor
135. Cloning and domain structure of the mammalian splicing factor U2AF
136. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit
137. Functional properties of p54, a novel SR protein active in constitutive and alternative splicing
138. Human homologs of yeast prp16 and prp17 reveal conservation of the mechanism for catalytic step II of pre-mRNA splicing
139. Both subunits of U2AF recognize the 3' splice site in Caenorhabditis elegans
140. 35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing
141. Functional domains of the human splicing factor ASF/SF2
142. The human splicing factor ASF/SF2 can specifically recognize pre-mRNA 5' splice sites