Determinants of SR protein specificity

Current Opinion in Cell Biology

Volumn 11, Issue 3, 1999, Pages 358-362

  Tacke, Roland ^a   Manley, James L ^a  

^a Och Spine at New York Presbyterian Hospitals   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA PRECURSOR; NUCLEAR FACTOR; PROTEIN;

METAZOON; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; REVIEW; RNA BINDING; RNA SPLICING;

METAZOA;

EID: 0033152209     PISSN: 09550674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0955-0674(99)80050-7     Document Type: Review

References (42)

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38. Wang J, Xiao SH, Manley JL: Genetic analysis of the SR protein ASF/SF2: Interchangeability of RS domains and negative control of splicing. Genes Dev 1998, 12:2222-2233. This paper employs a genetic complementation strategy to show that SR protein RS domains are interchangeable for ASF/SF2 functions required for viability of the chicken B-cell line DT40. By genetically depleting ASF/SF2, the study also provides first direct evidence that SR proteins can influence alternative splicing or negatively modulate splicing under physiological conditions.
39. Xiao SH, Manley JL: Phosphorylation-dephosphorylation differentially affects activities of splicing factor ASF/SF2. EMBO J 1998, 17:6359-6367.
40. Valcarcel J, Gaur RK, Singh R, Green MR: Interaction of U2AF65 RS region with pre-mRNA branch point and promotion of base pairing with U2 snRNA. Science 1996, 273:1706-1709.
41. Rudner DZ, Breger KS, Rio DC: Molecular genetic analysis of the heterodimeric splicing factor U2AF: The RS domain on either the large or small Drosophila subunit is dispensable in vivo. Genes Dev 1998, 12:1010-1021. This paper describes the unexpected observation that the RS domain of either subunit of the U2AF heterodimer can be deleted without affecting viability in Drosophila. Deletion of both RS domains, however, was lethal, suggesting either that the domains are redundant or that the cumulative effect of both deletions reduces U2AF activity below a threshold required for at least one essential function.
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