Functional properties of p54, a novel SR protein active in constitutive and alternative splicing

Molecular and Cellular Biology

Volumn 16, Issue 10, 1996, Pages 5400-5408

  Zhang, Wan Jiang ^a   Wu, Jane Y ^a  

^a WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MESSENGER RNA PRECURSOR; PHOSPHOPROTEIN; PROTEIN S 100; SMALL NUCLEAR RIBONUCLEOPROTEIN; VIRUS PROTEIN;

ALTERNATIVE RNA SPLICING; ARTICLE; CONTROLLED STUDY; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; PROTEIN RNA BINDING; SPLICEOSOME; TISSUE DISTRIBUTION;

EID: 0029843012     PISSN: 02707306     EISSN: None     Source Type: Journal    
DOI: 10.1128/MCB.16.10.5400     Document Type: Article

References (88)

1. Abovich, N., X.-L. Liao, and M. Rosbash. 1994. The yeast MUD2 protein: an interaction with PRP11 defines a bridge between commitment complexes and U2 snRNP addition. Genes Dev. 8:843-854.
2. Amrein, H., M. Gorman, and R. Nöthiger. 1988. The sex-determining gene tra-2 of Drosophila encodes a putative RNA binding protein. Cell 55:1025-1035.
3. Amrein, H., M. L. Hedley, and T. Maniatis. 1994. The role of specific protein-RNA and protein-protein interactions in positive and negative control of pre-mRNA splicing by Transformer 2. Cell 76:735-746.
4. Ayane, M., U, Preuss, G. Kohler, and P. J. Nielsen. 1991. A differentially expressed murine RNA encoding a protein with similarities to two types of nucleic acid binding motifs. Nucleic Acids Res. 19:1273-1278.
5. Baker, B. S. 1989. Sex in flies: the splice of life. Nature (London) 340:521-524.
6. Bennett, M., S. Michaud, J. Kingston, and R. Reed. 1992. Protein components specifically associated with prespliceosome and spliceosome complexes. Genes Dev. 6:1986-2000.
7. Berget, S. M. 1995. Exon recognition in vertebrate splicing. J. Biol. Chem. 2:2411-2414.
8. Birney, E., S. Kumar, and A. R. Krainer. 1993. Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors. Nucleic Acids Res. 21:5803-5816.
9. Black, D. L. 1995. Finding splice sites within a wilderness of RNA. RNA 1:763-771.
10. Blanar, M. A., and W. J. Kutter. 1992. Interaction cloning: identification of a helix-loop-helix zipper protein that interacts with c-Fos. Science 256:1014-1017.
11. Blencowe, B. J., R. Issner, J. Kim, P. McCaw, and P. A. Sharp. 1995. New proteins related to the Ser-Arg family of splicing factors. RNA 1:852-865.
12. Blencowe, B. J., J. A. Nickerson, R. Issner, S. Penman, and P. A. Sharp. 1994. Association of nuclear matrix antigens with exon-containing splicing complexes. J. Cell Biol. 127:593-607.
13. Boggs, R. T., P. Gregor, S. Idriss, J. M. Belote, and M. McKeown. 1987. Regulation of sexual differentiation in D. melanogaster via alternative splicing of RNA from the transformer gene. Cell 50:739-747.
14. Bruzik, J. P., and T. Maniatis. 1995. Enhancer-dependent interaction between 5′ and 3′ splice sites in trans. Proc. Natl. Acad. Sci. USA 92:7056-7059.
15. Cáceres, J. F., S. Stamm, D. M. Helfman, and A. R. Krainer. 1994. Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors. Science 265:1706-1709.
16. Cavaloc, Y., M. Popielarz, J. P. Fuchs, R. Gattoni, and J. Stevenin. 1994. Characterization and cloning of the human splicing factor 9G8: a novel 35 kDa factor of the serine/arginine protein family. EMBO J. 13:2639-2649.
17. Champlin, D. T., M. Frasch, H. Saumweber, and J. T. Lis. 1991. Characterization of a Drosophila protein associated with boundaries of transcriptionally active chromatin. Genes Dev. 5:1611-1621.
18. Chaudhary, N., C. McMahon, and G. Blobel. 1991. Primary structure of a human arginine-rich nuclear protein that colocalizes with spliceosome components. Proc. Natl. Acad. Sci. USA 88:8189-8193.
19. Chiara, M. D., and R. Heed. 1995. A two-step mechanism for 5′ and 3′ splice-site pairing. Nature (London) 375:510-513.
20. Crispino, J. D., B. J. Blencowe, and P. A. Sharp. 1993. Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science 265:1866-1869.
21. Diamond, R. H., K. Du, V. M. Lee, K. L. Mohn, B. A. Haber, D. S. Tewari, and R. Taub. 1993. Novel delayed-early and highly insulin-induced growth response genes: identification of HRS, a potential regulator of alternative pre-mRNA splicing. J. Biol. Chem. 268:15185-15192.
22. Dirksen, W. P., R. K. Hampson, Q. Sun, and F. M. Rottman. 1994. A purine-rich exon sequence enhances alternative splicing of bovine growth hormone pre-mRNA. J. Biol. Chem. 269:6431-6436.
23. Eperon, I. C., D. C. Ireland, R. A. Smith, A. Mayeda, and A. R. Krainer. 1993. Pathwavs for selection of 5′ splice sites by U1 snRNPs and SF2/ASF. EMBO J. 12:3607-3617.
24. Erba, H. P., P. Gunning, and L. Kedes. 1986. Nucleotide sequence of the human γ-cytoskeletal actin mRNA: anomalous evolution of vertebrate non-muscle actin genes. Nucleic Acids Res. 14:5275-5294.
25. Fu, X.-D. 1993. Specific commitment of different pre-mRNAs to splicing by single SR proteins. Nature (London) 365:82-85.
26. Fu, X.-D. 1995. The superfamily of arginine/serine rich splicing factors. RNA 1:663-680.
27. Fu, X.-D., and T. Maniatis. 1990. Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus. Nature (London) 343:437-441.
28. Fu, X.-D., and T. Maniatis. 1992. Isolation of a complementary DNA that encodes the mammalian splicing factor SC35. Science 256:535-538.
29. Fu, X. D., A. Mayeda, T. Maniatis, and A. Krainer. 1992. General splicing factors SF2 and SC35 have equivalent activities in vitro, and both affect alternative 5′ and 3′ splice site selection. Proc. Natl. Acad. Sci. USA 89: 11224-11228.
30. Ge, H., and J. L. Manley. 1990. A protein factor, ASF, controls cell-specific alternative splicing of SV40 early pre-mRNA in vitro. Cell 62:25-34.
31. Ge, H., P. Zuo, and J. L. Manley. 1991. Primary structure of the human splicing factor ASF reveals similarities with Drosophila regulators. Cell 66: 373-382.
32. Gilman, M. Z. 1988. Page 4.1.6. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology, vol. 1. John Wilery & Sons, New York.
33. Goralski, T. J., J.-E. Edström, and B. S. Baker. 1989. The sex determination locus transformer-2 of Drosophila encodes a polypeptide with similarity to RNA binding proteins. Cell 56:1011-1018.
34. Green, M. R. 1991. Biochemical mechanisms of constitutive and regulated pre-mRNA splicing. Annu. Rev. Cell Biol. 7:559-599.
35. Gui, J. F., W. S. Lane, and X.-D. Fu. 1994. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Nature (London) 369:678-682.
36. Kanaar, R., S. E. Roche, E. L. Beall, M. R. Green, and D. C. Rio. 1993. The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability. Science 262:569-573.
37. Kim, Y.-J., P. Zuo, J. L. Manley, and B. S. Baker. 1992. The Drosophila RNA-binding protein RBP1 is localized to transcription active sites of chromosomes and a functional similarity to human splicing factor ASF/SF2. Genes Dev. 6:2569-2579.
38. Kingston, R. E. 1988. Page 4.2.1. In F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl (ed.), Current protocols in molecular biology, vol. 1. John Wiley & Sons, New York.
39. Kohtz, J. D., S. F. Jamison, C. L. Will, P. Zuo, R. Lührmann, M. A. Garcia-Blanco, and J. L. Manley. 1994. Protein-protein interactions and 5′-splice-site recognition in mammalian mRNA precursors. Nature (London) 368: 119-124.
40. Krainer, A. R., G. C. Conway, and D. Kozak. 1990. Purification and characterization of pre-mRNA splicing factor SF2 from HcLa cells. Genes Dev. 4:1158-1171.
41. Krainer, A. R., G. C. Conway, and D. Kozak. 1990. The essential pre-mRNA splicing factor SF2 influences 5′ splice site selection by activating proximal sites. Cell 62:35-42.
42. Krainer, A. R., and T. Maniatis. 1985. Multiple factors including the small nuclear ribonucleoproteins U1 and U2 arc necessary for pre-mRNA splicing in vitro. Cell 42:725-736.
43. Krainer, A. R., T. Maniatis, B. Ruskin, and M. R. Green. 1984. Normal and mutant human β-globin pre-mRNAs are faithfully and efficiently spliced in vitro. Cell 36:993-1005.
44. Krainer, A. R., A. Mayeda, D. Kozak, and G. Binns. 1991. Functional expression of cloned human splicing factor SF2: homology to RNA-binding proteins, U1 70K, and Drosophila splicing regulators. Cell 66:383-394.
45. Kraus, M. E., and J. T. Lis. 1994. The concentration of B52, an essential splicing factor and regulator of splice site choice in vitro, is critical for Drosophila development. Mol. Cell. Biol. 14:5360-5370.
46. Lavigueur, A., H. La Branche, A. R. Kornblihtt, and B. Chabot. 1993. A splicing enhancer in the human fibronectin alternate ED1 exon interacts with SR proteins and stimulates U2 snRNP binding. Genes Dev. 7:2405-2417.
47. Legrain, P., and C. Chapon. 1993. Interaction between PRP11 and SPP91 yeast splicing factors and characterization of PRP9-PRP11-SPP91 complex. Science 262:108-110.
48. Li, H., and P. M. Bingham. 1991. Arginine/serine-rich domains of the su(wa) and tra RNA processing regulators target proteins to a subnuclear compartment implicated in splicing. Cell 67:335-342.
49. Lynch, K. W., and T. Maniatis. 1995. Synergistic interactions between two distinct elements of a regulated splicing enhancer. Genes Dev. 9:284-293.
50. Mayeda, A., and A. R. Krainer. 1992. Regulation of alternative pre-mRNA splicing by hnRNP A1 and splicing factor SF2. Cell 68:365-375.
51. Mayeda, A., A. M. Zahler, A. R. Krainer, and M. B. Roth. 1992. Two members of a conserved family of nuclear phosphoproteins are involved in pre-mRNA splicing. Proc. Natl. Acad. Sci. USA 89:1301-1304.
52. McKeown, M., J. M. Belote, and B. Baker. 1987. A molecular analysis of tra, a gene in D. melanogaster that controls female sexual differentiation. Cell 48:489-499.
53. Michaud, S., and R. Reed. 1993. A functional association between the 5′ and 3′ splice sites is established in the earliest prespliceosome complex (E) in mammals. Genes Dev. 7:1008-1020.
54. Moore, J. M., C. C. Query, and P. A. Sharp. 1993. Splicing of precursor to messenger RNAs by the spliceosome, p. 303-358. In R. F. Gesteland and J. F. Atkins (ed.), The RNA world. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
55. Peng, X.-B., and S. M. Mount. 1995. Genetic enhancement of RNA-processing defects by a dominant mutation in B52, the Drosophila gene for an SR protein splicing factor. Mol. Cell. Biol. 15:6273-6282.
56. Query, C. C., R. C. Bentley, and J. D. Keene. 1989. A common RNA recognition motif identified within a defined U1 RNA binding domain of the 70K U1 snRNP protein. Cell 57:89-101.
57. Ramchatesingh, J., A. M. Zahler, K. M. Neugebauer, M. B. Roth, and T. A. Cooper. 1995. A subset of SR proteins activates splicing of the cardiac troponin T alternative exon by direct interactions with an exonic enhancer. Mol. Cell. Biol. 15:4898-4907.
58. Reed, R., and T. Maniatis. 1986. A role for exon sequences and splice site proximity in splice site selection. Cell 46:681-690.
59. Ring, H.Z., and J. T. Lis. 1994. The SR protein B52/SRp55 is essential for Drosophila development. Mol. Cell. Biol. 14:7499-7506.
60. Rio, D. C. 1993. Splicing of pre-mRNA: mechanism, regulation and role in development. Curr. Opin. Genet. Dev. 3:574-584.
61. Rio, D. C. Personal communication.
62. Roscigno, R. F., and M. A. Garcia-Blanco. 1995. SR proteins escort U4/6.U5 tri-snRNP to the spliceosome. RNA 1:692-706.
63. Roth, M. B., C. Murphy, and J. G. Gall. 1990. A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle. J. Cell Biol. 111:2217-2223.
64. Ruskin, B., P. D. Zamore, and M. R. Green. 1988. A factor, U2AF, is required for U2 snRNP binding and splicing complex assembly. Cell 52:207-219.
65. Screaton, G. R., J. F. Cáceres, A. Mayeda, M. V. Bell, M. Plebanski, D. G. Jackson, J. I. Bell, and A. R. Krainer. 1995. Identification and characterization of three members of the human SR family of pre-mRNA splicing factors. EMBO J. 14:4336-4349.
66. Spritz, R. A., K. Strucnk, C. S. Surowy, S. O. Hoch, D. E. Barton, and U. Francke. 1987. The human U1-70k snRNP protein: cDNA cloning, chromosomal localization, expression, alternative splicing, and RNA-binding. Nucleic Acids Res. 15:10373-10391.
67. Staknis, D., and R. Reed. 1994. SR proteins promote the first specific recognition of pre-mRNA and are present together with the U1 small nuclear ribonucleoprotein particle in a general splicing enhancer complex. Mol. Cell. Biol. 14:7670-7682.
68. Sun, Q., A. Mayeda, R. K. Hampson, A. R. Krainer, and F. M. Rottman. 1993. General splicing factor SF2/ASF promotes alternative splicing by binding to an exonic splicing enhancer. Genes Dev. 7:2598-2608.
69. Tacke, R., and J. L. Manley. 1995. The human splicing factors ASF/SF2 and SC35 possess different, functionally significant RNA binding specificities. EMBO J. 14:3540-3551.
70. Tarn, W. Y., and J. A. Steitz. 1994. SR proteins can compensate for the loss of U1 snRNP functions in vitro. Genes Dev. 8:2704-2717.
71. Tanaka, K., A. Watakabe, and Y. Shimura. 1994. Polypurine sequences within a downstream exon function as a splicing enhancer. Mol. Cell. Biol. 14:1347-1354.
72. Theissen, H., M. Etzerodt, R. Reuter, C. Schneider, F. Lottspeich, P. Argos, R. Lührmann, and L. Fhilipson. 1986. Cloning of the human cDNA for the U1 RNA-associaled 70K protein. EMBO J. 5:3209-3217.
73. Tian, M., and T. Maniatis. 1993. A splicing enhancer complex controls alternative splicing of doublesex pre-mRNA. Cell 74:105-114.
74. Treisman, R., S. H. Orkin, and T. Maniatis. 1983. Specific transcription and RNA splicing defects in five cloned β-thalassaemia genes. Nature (London) 302:591-596.
75. Vellard, M., A. Sureau, J. Soret, C. Martinerie, and B. Perbal. 1992. A potential splicing factor is encoded by the opposite strand of the transspliced c-myb exon. Proc. Natl. Acad. Sci. USA 89:2511-2515.
76. Wang, J., and J. L. Manley. 1995. Overexpression of the SR proteins ASF/ SF2 and SC35 influences alternative splicing in vivo in diverse ways. RNA 1:335-346.
77. Wu, J. Y., and T. Maniatis. 1993. Specific interactions between proteins implicated in splice site selection and regulated alternative splicing. Cell 75:1061-1070.
78. Xu, R., J. Teng, and T. A. Cooper. 1993. The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element. Mol. Cell. Biol. 13:3660-3674.
79. Zahler, A. M., W. S. Lane, J. A. Stolk, and M. B. Roth. 1992. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6:837-847.
80. Zahler, A. M., K. M. Neugebauer, W. S. Lane, and M. B. Roth. 1993. Distinct functions of SR proteins in alternative pre-mRNA splicing. Science 260:219-222.
81. Zahler, A. M., K. M. Neugebauer, J. A. Stolk, and M. B. Roth. 1993. Human SR proteins and isolation of a cDNA encoding SRp75. Mol. Cell. Biol. 13: 4023-4028.
82. Zahler, A. M., and M. B. Roth. 1995. Distinct functions of SR proteins in recruitment of U1 small nuclear ribonucleoprotein to alternative 5′ splice sites. Proc. Natl. Acad. Sci. USA 92:2642-2646.
83. Zamore, P. D., and M. R. Green. 1989. Identification, purification and characterization of U2 small nuclear ribonuclcoprotein auxiliary factor. Proc. Natl. Acad. Sci. USA 86:9243-9247.
84. Zamore, P. D., and M. R. Green. 1991. Biochemical characterization of U2snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intracellular distribution. EMBO J. 10:207-214.
85. Zamore, P. D., J. G. Patton, and M. R. Green. 1992. Cloning and domain structure of the mammalian splicing factor U2AF. Nature (London) 355: 609-614.
86. Zervos, A. S., J. Gyuris, and R. Brent. 1993. Mix1, a protein that specifically interacts with Max to bind Myc-Max recognition sites. Cell 72:223-232.
87. Zhang, M., P. D. Zamore, M. Carmo-Fonseca, A. I. Lamond, and M. R. Green. 1992. Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit. Proc. Natl. Acad. Sci. USA 89:8769-8773.
88. 35 mediates critical protein-protein interactions in constitutive and enhancer-dependent splicing. Genes Dev. 10:1356-1368.