Initial splice-site recognition and pairing during pre-mRNA splicing

Current Opinion in Genetics and Development

Volumn 6, Issue 2, 1996, Pages 215-220

  Reed, Robin ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARGININE; MESSENGER RNA; SERINE; SMALL NUCLEAR RIBONUCLEOPROTEIN;

CONTROLLED STUDY; ENHANCER REGION; EXON; METAZOON; NONHUMAN; PRIORITY JOURNAL; PROTEIN RNA BINDING; RNA SEQUENCE; RNA SPLICING; SHORT SURVEY; SPLICEOSOME;

EID: 0029933504     PISSN: 0959437X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0959-437X(96)80053-0     Document Type: Article

References (47)

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32. ••,36-39] provide evidence that several different hnRNP proteins can play important roles in initial splice-site recognition.
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41. Chiara MD, Reed R: A two-step mechanism for 5′ and 3′ splice site pairing. Nature 1995, 375:510-513. RNAs can be spliced in trans if the 3′ splice site is associated with an exonic enhancer or downstream 5′ splice site. Selection of the trans 5′ splice site can occur after assembly of the A complex on the 3′ splice site, indicating that splice-site pairing can occur after initial splice-site recognition.
42. Konforti BB, Koziolkiewicz MJ, Konarska MM: Disruption of base-pairing between the 5′ splice site and the 5′ end of U1 snRNA is required for spliceosome assembly. Cell 1993, 75:863-873.
43. Konforti BB, Konarska MM: U4/U5/U6 snRNP recognizes the 5′ splice site in the absence of U2 snRNP. Genes Dev 1994, 8:1962-1973,
44. Sun J-S, Manley JL: A novel U2-U6 snRNA structure is necessary for mammalian mRNA splicing. Genes Dev 1995, 9:843-854. The essential U2-U6 interaction identified in yeast is shown to be required for RNA splicing in mammals. A novel U2-U6 helix, which may function in juxtaposing the branch site and 5′ splice site, was also detected.
45. Crispino JD, Blencowe BJ, Sharp PA: Complementation by SR proteins of pre-mRNA splicing reactions depleted of U1 snRNP. Science 1994, 265:1866-1869. Nuclear extracts specifically depleted of U1 snRNP can function in splicing if excess SR proteins are added to the reaction. This observation is consistent with the notion that multiple interactions, some of which can be dispensable, are involved in initial splice-site recognition.
46. ••].
47. Crispino JD, Sharp PA: A U6 snRNA:pre-mRNA interaction can be rate-limiting for U1-independent splicing. Genes Dev 1995, 9:2314-2323. Mutations in the 5′ splice site that increase complementarity to U6 snRNA facilitate splicing in the U1 snRNP-depleted extracts supplemented with SR proteins. This provides evidence that excess SR proteins obviate the requirement for U1 snRNP in early spliceosome assembly and that U6 snRNP recognizes the 5′ splice site later (as occurs on normal pre-mRNAs).