Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos

Science

Volumn 364, Issue 6437, 2019, Pages 289-292

  Zuo, Erwei ^a,b   Sun, Yidi ^c   Wei, Wu ^c,d,e   Yuan, Tanglong ^b   Ying, Wenqin ^a   Sun, Hao ^f   Yuan, Liyun ^c   Steinmetz, Lars M ^d,g   Li, Yixue ^c,h,i   Yang, Hui ^a  

^a Shenzhen Fundamental Research Institutions   (China)

^b INSTITUTE OF PLANT PROTECTION   (China)

^c UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

^d STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^e SHANGHAI JIAO TONG UNIVERSITY   (China)

^f UNIVERSITY OF CALIFORNIA   (United States)

^g EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^h FUDAN UNIVERSITY   (China)

ⁱ SHANGHAI CENTER FOR BIOINFORMATION TECHNOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

CELL; EMBRYO; EXPERIMENTAL STUDY; GENETIC ANALYSIS; GENETIC ENGINEERING; GENOMICS; MUTATION; POLYMORPHISM; RODENT;

ARTICLE; BLASTOMERE; CRISPR-CAS9 SYSTEM; EDITOR; EMBRYO; GENE MUTATION; HUMAN; HUMAN CELL; INJECTION; MOUSE EMBRYO; SINGLE NUCLEOTIDE POLYMORPHISM; SPONTANEOUS MUTATION; ANIMAL; BLASTOMA; C57BL MOUSE; CRISPR CAS SYSTEM; DNA MUTATIONAL ANALYSIS; FEMALE; GENE EDITING; GENOME-WIDE ASSOCIATION STUDY; MALE; MAMMALIAN EMBRYO; MOUSE; MUTATION; PROCEDURES;

CYTOSINE;

ANIMALS; BLASTOMERES; CRISPR-ASSOCIATED PROTEIN 9; CRISPR-CAS SYSTEMS; CYTOSINE; DNA MUTATIONAL ANALYSIS; EMBRYO, MAMMALIAN; FEMALE; GENE EDITING; GENOME-WIDE ASSOCIATION STUDY; MALE; MICE; MICE, INBRED C57BL; MUTATION; POLYMORPHISM, SINGLE NUCLEOTIDE;

EID: 85062990961     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aav9973     Document Type: Article

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