CRISPRCas9 and CRISPR-assisted cytidine deaminase enable precise and efficient genome editing in Klebsiella pneumoniae

Applied and Environmental Microbiology

Volumn 84, Issue 23, 2018, Pages

  Wang, Yu ^a   Wang, Shanshan ^b   Chen, Weizhong ^a   Song, Liqiang ^a   Zhang, Yifei ^a   Shen, Zhen ^c   Yu, Fangyou ^d   Li, Min ^c   Ji, Quanjiang ^a  

^a SHANGHAITECH UNIVERSITY   (China)

^b WENZHOU MEDICAL UNIVERSITY   (China)

^c SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

^d TONGJI UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

Base editing; Cas9; CRISPR; Genetic engineering; Genome editing; Klebsiella pneumoniae

Indexed keywords

BACTERIA; DNA; GENETIC ENGINEERING; HEALTH RISKS;

BASE EDITING; CAS9; CRISPR; CYTIDINE DEAMINASE; GENETIC MANIPULATIONS; KLEBSIELLA PNEUMONIAE; PNEUMONIAE STRAINS; RECOMBINATION SYSTEM;

GENES;

BACTERIUM; DETECTION METHOD; DRUG RESISTANCE; ENZYME; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; GENETICS; GENOME; MICROBIAL ACTIVITY; MICROORGANISM; PHYSIOLOGY; PROTEIN; PUBLIC HEALTH;

KLEBSIELLA PNEUMONIAE;

BACTERIAL PROTEIN; CYTIDINE DEAMINASE;

BACTERIAL GENOME; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CRISPR CAS SYSTEM; EVALUATION STUDY; GENE EDITING; GENETIC ENGINEERING; GENETICS; KLEBSIELLA PNEUMONIAE; METABOLISM; PROCEDURES;

BACTERIAL PROTEINS; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS; CRISPR-CAS SYSTEMS; CYTIDINE DEAMINASE; GENE EDITING; GENETIC ENGINEERING; GENOME, BACTERIAL; KLEBSIELLA PNEUMONIAE;

EID: 85056666811     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01834-18     Document Type: Article

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