A versatile one-step CRISPR-Cas9 based approach to plasmid-curing

Microbial Cell Factories

Volumn 16, Issue 1, 2017, Pages

  Lauritsen, Ida ^a   Porse, Andreas ^a   Sommer, Morten O A ^a   Nørholm, Morten H H ^a  

^a Chalmers University of Technology   (Denmark)

Author keywords

CRISPR Cas9; Genome engineering; PFREE; Plasmid curing; Pseudomonas putida; Replicon analysis

Indexed keywords

ARTICLE; BACTERIAL CELL; CLONING; CONTROLLED STUDY; CRISPR CAS SYSTEM; CRISPR CAS9 SYSTEM; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE EDITING; GENETIC ENGINEERING; GENETIC MANIPULATION; GENETIC PROCEDURES; GRAM NEGATIVE BACTERIUM; NONHUMAN; PLASMID; PLASMID CURING SYSTEM; POLYMERASE CHAIN REACTION; PSEUDOMONAS PUTIDA; REPLICON; SEQUENCE ANALYSIS; GENETICS; METABOLISM;

GREEN FLUORESCENT PROTEIN; GUIDE RNA;

CRISPR-CAS SYSTEMS; ESCHERICHIA COLI; GENETIC ENGINEERING; GREEN FLUORESCENT PROTEINS; PLASMIDS; PSEUDOMONAS PUTIDA; RNA, GUIDE;

EID: 85026638412     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-017-0748-z     Document Type: Article

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