Custom selenoprotein production enabled by laboratory evolution of recoded bacterial strains

Nature Biotechnology

Volumn 36, Issue 7, 2018, Pages 624-631

  Thyer, Ross ^a   Shroff, Raghav ^a   Klein, Dustin R ^b   D'Oelsnitz, Simon ^a   Cotham, Victoria C ^b   Byrom, Michelle ^a   Brodbelt, Jennifer S ^b   Ellington, Andrew D ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

^b University of Texas at Austin   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COVALENT BONDS; ESCHERICHIA COLI; RECOMBINANT PROTEINS; SULFUR COMPOUNDS;

ANTIBODY FRAGMENT; BACTERIAL STRAINS; DISULFIDE BONDS; FLUORESCENT REPORTER; INDUSTRIAL SCALE; REDUCING CONDITIONS; SELENOPROTEIN EXPRESSION; SYNTHETIC PEPTIDE;

SELENIUM COMPOUNDS;

BACTERIAL ENZYME; BETA LACTAMASE; BETA LACTAMASE NMC A; GREEN FLUORESCENT PROTEIN; SELENOCYSTEINE; SELENOPROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TRASTUZUMAB; UNCLASSIFIED DRUG; DISULFIDE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL FITNESS; BACTERIAL PHENOMENA AND FUNCTIONS; BACTERIAL STRAIN; CHEMICAL BOND; CONTROLLED STUDY; DISELENIDE BOND; DISULFIDE BOND; ESCHERICHIA COLI; EVOLUTION; FLUORESCENCE; GENETIC CODE; HOST; IN VIVO STUDY; LABORATORY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN STABILITY; PROTEIN SYNTHESIS; STOP CODON; WHOLE GENOME SEQUENCING; BIOSYNTHESIS; CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; GENE EXPRESSION REGULATION; GENETICS;

BETA-LACTAMASES; DIRECTED MOLECULAR EVOLUTION; DISULFIDES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; SELENOCYSTEINE; SELENOPROTEINS;

EID: 85049754558     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt.4154     Document Type: Article

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