High-level expression in Escherichia coli of selenocysteine-containing rat thioredoxin reductase utilizing gene fusions with engineered bacterial-type SECIS elements and co-expression with the selA, selB and selC genes

Journal of Molecular Biology

Volumn 292, Issue 5, 1999, Pages 1003-1016

  Arnér, Elias S J ^a   Sarioglu, Hakan ^b   Lottspeich, Friedrich ^b   Holmgren, Arne ^a   Böck, August ^b  

^a KAROLINSKA INSTITUTE   (Sweden)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Expression; Heterologous; SECIS element; Selenocysteine; Thioredoxin reductase

Indexed keywords

BACTERIAL PROTEIN; FORMATE DEHYDROGENASE; MESSENGER RNA; RECOMBINANT PROTEIN; SELENOCYSTEINE; SELENOPROTEIN; THIOL; THIOREDOXIN REDUCTASE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENETICS; CELL PROLIFERATION; CODON; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME PURIFICATION; ESCHERICHIA COLI; GENE EXPRESSION; GENE FUSION; GENE INSERTION; GENETIC ENGINEERING; NONHUMAN; OPEN READING FRAME; OXIDATION REDUCTION REACTION; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; PROTEIN SYNTHESIS; SPECIES DIFFERENCE; TRANSLATION REGULATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA; NEGIBACTERIA;

EID: 0033536575     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.1999.3085     Document Type: Article

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