Isolation of a human-like antibody fragment (scFv) that neutralizes ricin biological activity

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Pelat, Thibaut ^a   Hust, Michael ^b   Hale, Martha ^c   Lefranc, Marie Paule ^d,e   Dübel, Stefan ^b   Thullier, Philippe ^a  

^a CENTRE DE RECHERCHES   (France)

^b TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^c UNITED STATES ARMY MEDICAL RESEARCH INSTITUTE OF INFECTIOUS DISEASES   (United States)

^d CNRS and University Montpellier II   (France)

^e INSTITUT UNIVERSITAIRE DE FRANCE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY FRAGMENT; BIOLOGICAL ACTIVITIES; BIOWEAPON; CHARACTERIZATION STUDIES; GERMLINE GENES; HUMAN IGG; LIGHT CHAIN; MOLAR RATIO; MOUSE SERUM; NON-HUMAN PRIMATE; POLYCLONAL; PROTEIN SYNTHESIS; RICINUS COMMUNIS; SEQUENCE IDENTITY;

AMINES; AMINO ACIDS; CLONING; GENES; ORGANIC ACIDS;

ANTIBODIES;

IMMUNOGLOBULIN G; IMMUNOGLOBULIN LAMBDA CHAIN; NEUTRALIZING ANTIBODY; RICIN A; RICIN ANTIBODY; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; IMMUNOGLOBULIN FRAGMENT; PEPTIDE LIBRARY; RICIN;

AMINO ACID COMPOSITION; ANIMAL EXPERIMENT; ANTIBODY ISOLATION; ANTIBODY LIBRARY; ANTIBODY SCREENING; ANTIBODY SPECIFICITY; ANTIBODY STRUCTURE; ANTIGEN ANTIBODY REACTION; ANTIGEN BINDING; ARTICLE; BINDING AFFINITY; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; DEGLYCOSYLATION; DRUG DETERMINATION; DRUG ISOLATION; IMMUNIZATION; MACACA; MALE; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; SEQUENCE HOMOLOGY; SERODIAGNOSIS; VACCINE PRODUCTION; AMINO ACID SEQUENCE; ANIMAL; ANTIBODY AFFINITY; DRUG ANTAGONISM; GENE LIBRARY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; SENSITIVITY AND SPECIFICITY;

MACACA FASCICULARIS; PRIMATES; RICINUS COMMUNIS;

AMINO ACID SEQUENCE; ANIMALS; ANTIBODY AFFINITY; GENE LIBRARY; HUMANS; IMMUNOGLOBULIN FRAGMENTS; MACACA FASCICULARIS; MALE; MOLECULAR SEQUENCE DATA; NEUTRALIZATION TESTS; PEPTIDE LIBRARY; RICIN; SENSITIVITY AND SPECIFICITY;

EID: 67849092221     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-60     Document Type: Article

References (44)

1. Olsnes S Pihl A Ricin - a potent inhibitor of protein synthesis FEBS Lett 1972, 20(3):327-329 11946449
2. Endo Y Tsurugi K RNA N-glycosidase activity of ricin A-chain. Mechanism of action of the toxic lectin ricin on eukaryotic ribosomes J Biol Chem 1987, 262(17):8128-8130 3036799
3. Audi J Belson M Patel M Schier J Osterloh J Ricin poisoning: A comprehensive review Jama 2005, 294(18):2342-2351 16278363
4. Balint GA Ricin: The toxic protein of castor oil seeds Toxicology 1974, 2(1):77-102 4823740
5. Fodstad O Olsnes S Pihl A Toxicity, distribution and elimination of the cancerostatic lectins abrin and ricin after parenteral injection into mice Br J Cancer 1976, 34(4):418-425 974006
6. Knight B Ricin - a potent homicidal poison Br Med J 1979, 1(6159):350-351 421122
7. Olsnes S The history of ricin, abrin and related toxins Toxicon 2004, 44(4):361-370 15302520
8. Roy CJ Hale M Hartings JM Pitt L Duniho S Impact of inhalation exposure modality and particle size on the respiratory deposition of ricin in BALB/c mice Inhal Toxicol 2003, 15(6):619-638 12692733
9. Mayor S UK doctors warned after ricin poison found in police raid Bmj 2003, 326(7381):126 1169074 12531838
10. Doan LG Ricin: Mechanism of toxicity, clinical manifestations, and vaccine development. A review J Toxicol Clin Toxicol 2004, 42(2):201-208 15214627
11. Zilinskas RA Iraq's biological weapons. The past as future? Jama 1997, 278(5):418-424 9244334
12. Guglielmo-Viret V Splettstoesser W Thullier P An immunochromatographic test for the diagnosis of ricin inhalational poisoning Clin Toxicol (Phila) 2007, 45(5):505-511 17503256
13. Liu JL Anderson GP Goldman ER Isolation of anti-toxin single domain antibodies from a semi-synthetic spiny dogfish shark display library BMC Biotechnol 2007, 7:78 2213646 18021450
14. Burnett JC Henchal EA Schmaljohn AL Bavari S The evolving field of biodefence: Therapeutic developments and diagnostics Nat Rev Drug Discov 2005, 4(4):281-297 15803193
15. Miller DJ Ravikumar K Shen H Suh JK Kerwin SM Robertus JD Structure-based design and characterization of novel platforms for ricin and shiga toxin inhibition J Med Chem 2002, 45(1):90-98 11754581
16. Smallshaw JE Richardson JA Vitetta ES RiVax, a recombinant ricin subunit vaccine, protects mice against ricin delivered by gavage or aerosol Vaccine 2007, 25(42):7459-7469 2049008 17875350
17. Poli MA Rivera VR Pitt ML Vogel P Aerosolized specific antibody protects mice from lung injury associated with aerosolized ricin exposure Toxicon 1996, 34(9):1037-1044 8896195
18. Foxwell BM Detre SI Donovan TA Thorpe PE The use of anti-ricin antibodies to protect mice intoxicated with ricin Toxicology 1985, 34(1):79-88 3969682
19. Pratt TS Pincus SH Hale ML Moreira AL Roy CJ Tchou-Wong KM Oropharyngeal aspiration of ricin as a lung challenge model for evaluation of the therapeutic index of antibodies against ricin A-chain for post-exposure treatment Exp Lung Res 2007, 33(8-9):459-481 17994372
20. Wang Y Guo L Zhao K Chen J Feng J Sun Y Li Y Shen B Novel chimeric anti-ricin antibody C4C13 with neutralizing activity against ricin toxicity Biotechnol Lett 2007, 29(12):1811-1816 17657413
21. Pelat T Hust M Laffly E Condemine F Bottex C Vidal D Lefranc MP Dubel S Thullier P High-affinity, human antibody-like antibody fragment (single-chain variable fragment) neutralizing the lethal factor (LF) of Bacillus anthracis by inhibiting protective antigen-LF complex formation Antimicrob Agents Chemother 2007, 51(8):2758-2764 1932538 17517846
22. Laffly E Danjou L Condemine F Vidal D Drouet E Lefranc MP Bottex C Thullier P Selection of a macaque Fab with framework regions like those in humans, high affinity, and ability to neutralize the protective antigen (PA) of Bacillus anthracis by binding to the segment of PA between residues 686 and 694 Antimicrob Agents Chemother 2005, 49(8):3414-3420 1196273 16048955
23. Chassagne S Laffly E Drouet E Herodin F Lefranc MP Thullier P A high-affinity macaque antibody Fab with human-like framework regions obtained from a small phage display immune library Mol Immunol 2004, 41(5):539-546 15183932
24. Pelat T Bedouelle H Rees AR Crennell SJ Lefranc MP Thullier P Germline Humanization of a Non-human Primate Antibody that Neutralizes the Anthrax Toxin, by in Vitro and in Silico Engineering J Mol Biol 2008, 384(5):1400-7 18976662
25. Carter P Bedouelle H Winter G Improved oligonucleotide site-directed mutagenesis using M13 vectors Nucleic Acids Res 1985, 13(12):4431-4443 321798 2989795
26. Pommie C Levadoux S Sabatier R Lefranc G Lefranc MP IMGT standardized criteria for statistical analysis of immunoglobulin V-REGION amino acid properties J Mol Recognit 2004, 17(1):17-32 14872534
27. McGuinness CR Mantis NJ Characterization of a novel high-affinity monoclonal immunoglobulin G antibody against the ricin B subunit Infect Immun 2006, 74(6):3463-3470 1479246 16714577
28. Hust M Dubel S Schirrmann T Selection of recombinant antibodies from antibody gene libraries Methods Mol Biol 2007, 408:243-255 18314587
29. Kirsch MI Hulseweh B Nacke C Rulker T Schirrmann T Marschall HJ Hust M Dubel S Development of human antibody fragments using antibody phage display for the detection and diagnosis of Venezuelan equine encephalitis virus (VEEV) BMC Biotechnol 2008, 8:66 2543005 18764933
30. Sambrook J Russell DW Molecular cloning: A laboratory manual Cold Spring Harbor, N.Y.: Cold Spring Harbor Laboratory Press 3 2001
31. Hust M Meysing M Schirrmann T Selke M Meens J Gerlach GF Dubel S Enrichment of open reading frames presented on bacteriophage M13 using hyperphage Biotechniques 2006, 41(3):335-342 16989094
32. Soltes G Hust M Ng KK Bansal A Field J Stewart DI Dubel S Cha S Wiersma EJ On the influence of vector design on antibody phage display J Biotechnol 2007, 127(4):626-637 1866265 16996161
33. Kugler J Nieswandt S Gerlach GF Meens J Schirrmann T Hust M Identification of immunogenic polypeptides from a Mycoplasma hyopneumoniae genome library by phage display Appl Microbiol Biotechnol 2008, 80(3):447-458 18636254
34. Hust M Jostock T Menzel C Voedisch B Mohr A Brenneis M Kirsch MI Meier D Dubel S Single chain Fab (scFab) fragment BMC Biotechnol 2007, 7:14 1829395 17346344
35. Andris-Widhopf J Rader C Steinberger P Fuller R Barbas CF 3rd Methods for the generation of chicken monoclonal antibody fragments by phage display J Immunol Methods 2000, 242(1-2):159-181 10986398
36. Rader C Steinberger P Barbas CF 3rd Library panning on immobilized antigens Phage display A laboratory manual Silverman. New York: CSHL press Barbas CF 3rd, DR Burton JK, Scott GJ 2001:10.12-10.15
37. Renard M Belkadi L Hugo N England P Altschuh D Bedouelle H Knowledge-based design of reagentless fluorescent biosensors from recombinant antibodies J Mol Biol 2002, 318(2):429-442 12051849
38. Hale ML Microtitre-based assay for evaluating the biological activity of ribosome-inactivating proteins Pharmacol Toxicol 2001, 88(5):255-260 11393586
39. Karlsson R Michaelsson A Mattsson L Kinetic analysis of monoclonal antibody-antigen interactions with a new biosensor based analytical system J Immunol Methods 1991, 145(1-2):229-240 1765656
40. Schuck P Minton AP Kinetic analysis of biosensor data: Elementary tests for self-consistency Trends Biochem Sci 1996, 21(12):458-460 9009825
41. Kirsch M Zaman M Meier D Dubel S Hust M Parameters affecting the display of antibodies on phage J Immunol Methods 2005, 301(1-2):173-185 15992816
42. Lefranc MP IMGT, the international ImMunoGeneTics database Nucleic Acids Res 2003, 31(1):307-310 165532 12520009
43. Giudicelli V Duroux P Ginestoux C Folch G Jabado-Michaloud J Chaume D Lefranc MP IMGT/LIGM-DB, the IMGT comprehensive database of immunoglobulin and T cell receptor nucleotide sequences Nucleic Acids Res 2006, 34: Database D781-784 1347451 16381979
44. Monod MY Giudicelli V Chaume D Lefranc MP IMGT/JunctionAnalysis: The first tool for the analysis of the immunoglobulin and T cell receptor complex V-J and V-D-J JUNCTIONs Bioinformatics 2004, 20(Suppl 1):I379-I385 15262823