Characterization and structural analysis of human selenium-dependent glutathione peroxidase 4 mutant expressed in Escherichia coli

Free Radical Biology and Medicine

Volumn 71, Issue , 2014, Pages 332-338

  Yu, Yang ^a   Song, Jian ^b   Guo, Xiao ^a   Wang, Shuan ^a   Yang, Xiao ^a   Chen, Long ^a   Wei, Jingyan ^a  

^a JILIN UNIVERSITY   (China)

^b College of Electronic Science and Engineering   (China)

Author keywords

Antioxidants; Cys auxotrophic strain; Free radicals; Glutathione peroxidase 4; Mutants; Overexpression; Selenoenzymes

Indexed keywords

AMINO ACID; CYS 107; CYS 148; CYS 2; CYS 37; CYS 75; CYSTEINE; PHOSPHOLIPID HYDROPEROXIDE GLUTATHIONE PEROXIDASE; SELENIUM; SELENOCYSTEINE; UNCLASSIFIED DRUG; GLUTATHIONE PEROXIDASE; PHOSPHOLIPID-HYDROPEROXIDE GLUTATHIONE PEROXIDASE; RECOMBINANT PROTEIN; SERINE;

AMINO ACID SEQUENCE; ARTICLE; BACTERIAL MUTATION; BIOLOGICAL ACTIVITY; CATALYSIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ESCHERICHIA COLI; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; RESIDUE ANALYSIS; STEADY STATE; STOP CODON; STRUCTURE ANALYSIS; SUBSTITUTION REACTION; CHEMICAL STRUCTURE; CHEMISTRY; GENE EXPRESSION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MUTATION; PLASMID; PROTEIN ENGINEERING;

CLONING, MOLECULAR; CYSTEINE; ESCHERICHIA COLI; GENE EXPRESSION; GLUTATHIONE PEROXIDASE; HUMANS; MODELS, MOLECULAR; MUTATION; PLASMIDS; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; SELENOCYSTEINE; SERINE;

EID: 84898826109     PISSN: 08915849     EISSN: 18734596     Source Type: Journal    
DOI: 10.1016/j.freeradbiomed.2014.03.032     Document Type: Article

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