Amino acid sequence requirements at residues 69 and 238 for the SME-1 β-lactamase to confer resistance to β-lactam antibiotics

Antimicrobial Agents and Chemotherapy

Volumn 47, Issue 3, 2003, Pages 1062-1067

  Majiduddin, Fahd K ^a   Palzkill, Timothy ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN; BACTERIAL ENZYME; BETA LACTAM ANTIBIOTIC; BETA LACTAMASE; CARBAPENEM; CEFOTAXIME; CHLORAMPHENICOL; CYSTEINE; IMI 1 BETA LACTAMASE; IMIPENEM; KPC 1 BETA LACTAMASE; NMC A BETA LACTAMASE; SME 1 BETA LACTAMASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL MUTATION; CATALYSIS; CONCENTRATION RESPONSE; CONTROLLED STUDY; DISULFIDE BOND; DRUG HYDROLYSIS; ENZYME ANALYSIS; ESCHERICHIA COLI; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; BETA-LACTAM RESISTANCE; BETA-LACTAMASES; CARBAPENEMS; CEPHALOSPORINS; CLONING, MOLECULAR; CODON; DISULFIDES; ESCHERICHIA COLI; GENE LIBRARY; HYDROLYSIS; MICROBIAL SENSITIVITY TESTS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PENICILLINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 0037416972     PISSN: 00664804     EISSN: None     Source Type: Journal    
DOI: 10.1128/AAC.47.3.1062-1067.2003     Document Type: Article

References (20)

1. Bradley, J. S., J. Garau, H. Lode, K. V. Rolston, S. E. Wilson, and J. P. Quinn. 1999. Carbapenems in clinical practice: a guide to their use in serious infection. Int. J. Antimicrob. Agents 11:93-100.
2. Bullock, W. O., J. M. Fernandez, and J. M. Short. 1987. XL-1 Blue: a high efficiency plasmid transforming recA Eschericia coli strain with β-galactosidase selection. BioTechniques 5:376-379.
3. Hellinger, W. C., and N. S. Brewer. 1999. Carbapenems and monobactams: imipenem, meropenem, and aztreonam. Mayo Clin. Proc. 74:420-434.
4. Ho, S. N., H. D. Hunt, R. M. Horton, J. K. Pullen, and L. R. Pease. 1989. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77:51-59.
5. Koshland, D. E., Jr. 1998. Conformational changes: how small is big enough? Nat. Med. 4:1112-1114.
6. Livermore, D. M., and N. Woodford. 2000. Carbepenemases: a problem in waiting? Curr. Opin. Microbiol. 3:489-495.
7. Naas, T., and P. Nordmann. 1994. Analysis of a carbapenem-hydrolyzing class A beta-lactamase from Enterobacter cloacae and of its LysR-type regulatory protein. Proc. Natl. Acad. Sci. USA 91:7693-7697.
8. Naas, T., L. Vandel, W. Sougakoff, D. M. Livermore, and P. Nordmann. 1994. Cloning and sequence analysis of the gene for a carbapenem-hydrolyzing class A beta-lactamase, SME-1, from Serratia marcescens S6. Antimicrob. Agents Chemother. 38:1262-1270.
9. Nordmann, P., and L. Poirel. 2002. Emerging carbapenemases in gram-negative aerobes. Clin. Microbiol. Infect. 8:321-331.
10. Petrosino, J., G. W. Rudgers, H. Gilbert, and T. Palzkill. 1999. Contribution of aspartate 49 and phenylalanine 142 residues of a tight binding inhibitory protein of β-lactamases. J. Biol. Chem. 274:2394-2400.
11. Poirel, L., and P. Nordmann. 2002. Acquired carbapenem-hydrolyzing beta-lactamases and their genetic support. Curr. Pharm. Biotechnol. 3:117-127.
12. Raquet, X., J. Lamotte-Brasseur, F. Bouillenne, and J.-M. Frere. 1997. A disulfide bridge near the active site of carbapenem hydrolyzing class A β-lactamases might explain their unusual substrate profile. Proteins 27:47-58.
13. Rasmussen, B. A., K. Bush, D. Keeney, Y. Yang, R. Hare, C. O'Gara, and A. A. Medeiros. 1996. Characterization of IMI-1 β-lactamase, a class A carbapenem-hydrolyzing enzyme from Enterobacter cloacae. Antimicrob. Agents Chemother. 40:2080-2086.
14. Schultz, S. C., G. Dalbadie-McFarland, J. J. Neitzel, and J. H. Richards. 1987. Stability of wild-type and mutant RTEM-1 beta-lactamases: effect of the disulfide bond. Proteins 2:290-297.
15. Sougakoff, W., G. L'Hermite, L. Pernot, T. Naas, V. Guillet, P. Nordmann, V. Jarlier, and J. Delettre. 2002. Structure of the imipenem-hydrolyzing class A beta-lactamase SME-1 from Serratia marcescens. Acta Crystallogr. Sect. D Biol. Crystallogr. 58(Pt 2):267-274.
16. Sougakoff, W., T. Naas, P. Nordmann, E. Collatz, and V. Jarlier. 1999. Role of Ser-237 in the substrate specificity of the carbapenem-hydrolyzing class A beta-lactamase SME-1. Biochim. Biophys. Acta 1433:153-158.
17. Swaren, P., L. Maveyraud, X. Raquet, S. Cabantous, C. Duez, J.-D. Pedelacq, S. Mariotte-Boyer, L. Mourey, R. Labia, M.-H. Nicolas-Chanoine, P. Nordmann, J.-M. Frere, and J.-P. Samama. 1998. X-ray analysis of the NMC-A β-lactamase at 1.64-Å resolution, a class A carbapenemase with broad spectrum specificity. J. Biol. Chem. 273:26714-26721.
18. Vanhove, M., G. Guillaume, P. Ledent, J. H. Richards, R. H. Pain, and J. M. Frere. 1997. Kinetic and thermodynamic consequences of the removal of the Cys-77 - Cys-123 disulphide bond for the folding of TEM-1 beta-lactamase. Biochem. J. 321(Pt 2):413-417.
19. Waldburger, C. D., J. F. Schildbach, and R. T. Sauer. 1995. Are buried salt bridges important for protein stability and conformational specificity? Nat. Struct. Biol. 2:122-128.
20. Yigit, H., A. M. Queenan, G. J. Anderson, A. Domenech-Sanchez, J. W. Biddle, C. D. Steward, S. Alberti, K. Bush, and F. C. Tenover. 2001. Novel carbapenem-hydrolyzing beta-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob. Agents Chemother. 45:1151-1161.