Systematic screening of soluble expression of antibody fragments in the cytoplasm of E. coli

Microbial Cell Factories

Volumn 15, Issue 1, 2016, Pages

  Gaciarz, Anna ^a   Veijola, Johanna ^a   Uchida, Yuko ^a   Saaranen, Mirva J ^a   Wang, Chunguang ^a   Hörkkö, Sohvi ^a,b   Ruddock, Lloyd W ^a  

^a UNIVERSITY OF OULU   (Finland)

^b OULU UNIVERSITY HOSPITAL   (Finland)

Author keywords

(3 10): Antibody fragments; Cytoplasm; Disulfide bonds; Escherichia coli; Fab; ScFv

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; ANTIBODY; IMMUNOGLOBULIN FRAGMENT;

ANTIBODY STRUCTURE; ARTICLE; BACTERIAL CELL; CIRCULAR DICHROISM; CONTROLLED STUDY; CYTOPLASM; DISULFIDE BOND; ESCHERICHIA COLI; HUMAN; ISOMERIZATION; MOUSE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; ANIMAL; ELECTROSPRAY MASS SPECTROMETRY; GENE VECTOR; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR WEIGHT; PLASMID; PROCEDURES; PROTEIN ENGINEERING; SOLUBILITY;

ANIMALS; ANTIBODIES; CYTOPLASM; ESCHERICHIA COLI; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN FRAGMENTS; MICE; MOLECULAR WEIGHT; PLASMIDS; PROTEIN ENGINEERING; SINGLE-CHAIN ANTIBODIES; SOLUBILITY; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

EID: 84959138693     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-016-0419-5     Document Type: Article

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