-
1
-
-
84955581565
-
The development of cryo-EM into a mainstream structural biology technique
-
Nogales E (2016) The development of cryo-EM into a mainstream structural biology technique. Nat Methods 13:24–27.
-
(2016)
Nat Methods
, vol.13
, pp. 24-27
-
-
Nogales, E.1
-
2
-
-
84955582151
-
How good can cryo-EM become?
-
Glaeser RM (2016) How good can cryo-EM become? Nat Methods 13:28–32.
-
(2016)
Nat Methods
, vol.13
, pp. 28-32
-
-
Glaeser, R.M.1
-
3
-
-
84969983828
-
Breaking cryo-EM resolution barriers to facilitate drug discovery
-
Merk A, et al. (2016) Breaking cryo-EM resolution barriers to facilitate drug discovery. Cell 165:1698–1707.
-
(2016)
Cell
, vol.165
, pp. 1698-1707
-
-
Merk, A.1
-
4
-
-
84924617498
-
2.8-Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy
-
Campbell MG, Veesler D, Cheng A, Potter CS, Carragher B (2015) 2.8-Å resolution reconstruction of the Thermoplasma acidophilum 20S proteasome using cryo-electron microscopy. eLife 4:e06380.
-
(2015)
eLife
, vol.4
, pp. e06380
-
-
Campbell, M.G.1
Veesler, D.2
Cheng, A.3
Potter, C.S.4
Carragher, B.5
-
5
-
-
84969627248
-
TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action
-
Gao Y, Cao E, Julius D, Cheng Y (2016) TRPV1 structures in nanodiscs reveal mechanisms of ligand and lipid action. Nature 534:347–351.
-
(2016)
Nature
, vol.534
, pp. 347-351
-
-
Gao, Y.1
Cao, E.2
Julius, D.3
Cheng, Y.4
-
6
-
-
84958883175
-
2.3-Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition
-
Banerjee S, et al. (2016) 2.3-Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition. Science 351:871–875.
-
(2016)
Science
, vol.351
, pp. 871-875
-
-
Banerjee, S.1
-
7
-
-
85019070009
-
An atomic structure of the human spliceosome
-
e14
-
Zhang X, et al. (2017) An atomic structure of the human spliceosome. Cell 169: 918–929.e14.
-
(2017)
Cell
, vol.169
, pp. 918-929
-
-
Zhang, X.1
-
8
-
-
85029782310
-
The cryo-electron microscopy structure of human transcription factor IIH
-
Greber BJ, et al. (2017) The cryo-electron microscopy structure of human transcription factor IIH. Nature 549:414–417.
-
(2017)
Nature
, vol.549
, pp. 414-417
-
-
Greber, B.J.1
-
9
-
-
85032875994
-
Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3
-
Hirschi M, et al. (2017) Cryo-electron microscopy structure of the lysosomal calcium-permeable channel TRPML3. Nature 550:411–414.
-
(2017)
Nature
, vol.550
, pp. 411-414
-
-
Hirschi, M.1
-
10
-
-
0035956861
-
Nanohedra: Using symmetry to design self-assembling protein cages, layers, crystals, and filaments
-
Padilla JE, Colovos C, Yeates TO (2001) Nanohedra: Using symmetry to design self-assembling protein cages, layers, crystals, and filaments. Proc Natl Acad Sci USA 98: 2217–2221.
-
(2001)
Proc Natl Acad Sci USA
, vol.98
, pp. 2217-2221
-
-
Padilla, J.E.1
Colovos, C.2
Yeates, T.O.3
-
11
-
-
84861652462
-
Structure of a 16-nm cage designed by using protein oligomers
-
Lai Y-T, Cascio D, Yeates TO (2012) Structure of a 16-nm cage designed by using protein oligomers. Science 336:1129.
-
(2012)
Science
, vol.336
, pp. 1129
-
-
Lai, Y.-T.1
Cascio, D.2
Yeates, T.O.3
-
12
-
-
84877588832
-
Self-assembling cages from coiled-coil peptide modules
-
Fletcher JM, et al. (2013) Self-assembling cages from coiled-coil peptide modules. Science 340:595–599.
-
(2013)
Science
, vol.340
, pp. 595-599
-
-
Fletcher, J.M.1
-
13
-
-
84874061841
-
Re-engineering protein interfaces yields copper-inducible ferritin cage assembly
-
Huard DJE, Kane KM, Tezcan FA (2013) Re-engineering protein interfaces yields copper-inducible ferritin cage assembly. Nat Chem Biol 9:169–176.
-
(2013)
Nat Chem Biol
, vol.9
, pp. 169-176
-
-
Huard, D.J.E.1
Kane, K.M.2
Tezcan, F.A.3
-
14
-
-
84902006780
-
Accurate design of co-assembling multi-component protein nanomaterials
-
King NP, et al. (2014) Accurate design of co-assembling multi-component protein nanomaterials. Nature 510:103–108.
-
(2014)
Nature
, vol.510
, pp. 103-108
-
-
King, N.P.1
-
15
-
-
84979578745
-
Accurate design of megadalton-scale two-component icosahedral protein complexes
-
Bale JB, et al. (2016) Accurate design of megadalton-scale two-component icosahedral protein complexes. Science 353:389–394.
-
(2016)
Science
, vol.353
, pp. 389-394
-
-
Bale, J.B.1
-
16
-
-
84982076183
-
Flexible, symmetry-directed approach to assembling protein cages
-
Sciore A, et al. (2016) Flexible, symmetry-directed approach to assembling protein cages. Proc Natl Acad Sci USA 113:8681–8686.
-
(2016)
Proc Natl Acad Sci USA
, vol.113
, pp. 8681-8686
-
-
Sciore, A.1
-
17
-
-
0042780350
-
Designing repeat proteins: Well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins
-
Binz HK, Stumpp MT, Forrer P, Amstutz P, Plückthun A (2003) Designing repeat proteins: Well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins. J Mol Biol 332:489–503.
-
(2003)
J Mol Biol
, vol.332
, pp. 489-503
-
-
Binz, H.K.1
Stumpp, M.T.2
Forrer, P.3
Amstutz, P.4
Plückthun, A.5
-
18
-
-
49449118042
-
Light-activated DNA binding in a designed allosteric protein
-
Strickland D, Moffat K, Sosnick TR (2008) Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA 105:10709–10714.
-
(2008)
Proc Natl Acad Sci USA
, vol.105
, pp. 10709-10714
-
-
Strickland, D.1
Moffat, K.2
Sosnick, T.R.3
-
19
-
-
84855513111
-
Systematic control of protein interaction using a modular ER/K α-helix linker
-
Sivaramakrishnan S, Spudich JA (2011) Systematic control of protein interaction using a modular ER/K α-helix linker. Proc Natl Acad Sci USA 108:20467–20472.
-
(2011)
Proc Natl Acad Sci USA
, vol.108
, pp. 20467-20472
-
-
Sivaramakrishnan, S.1
Spudich, J.A.2
-
20
-
-
84962265255
-
DARPin-based crystallization chaperones exploit molecular geometry as a screening dimension in protein crystallography
-
Batyuk A, Wu Y, Honegger A, Heberling MM, Plückthun A (2016) DARPin-based crystallization chaperones exploit molecular geometry as a screening dimension in protein crystallography. J Mol Biol 428:1574–1588.
-
(2016)
J Mol Biol
, vol.428
, pp. 1574-1588
-
-
Batyuk, A.1
Wu, Y.2
Honegger, A.3
Heberling, M.M.4
Plückthun, A.5
-
21
-
-
84957709564
-
Generation of fluorogen-activating designed ankyrin repeat proteins (FADAs) as versatile sensor tools
-
Schütz M, et al. (2016) Generation of fluorogen-activating designed ankyrin repeat proteins (FADAs) as versatile sensor tools. J Mol Biol 428:1272–1289.
-
(2016)
J Mol Biol
, vol.428
, pp. 1272-1289
-
-
Schütz, M.1
-
22
-
-
85029379855
-
Rigidly connected multispecific artificial binders with adjustable geometries
-
Wu Y, et al. (2017) Rigidly connected multispecific artificial binders with adjustable geometries. Sci Rep 7:11217.
-
(2017)
Sci Rep
, vol.7
, pp. 11217
-
-
Wu, Y.1
-
23
-
-
21744440783
-
Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins
-
Amstutz P, et al. (2005) Intracellular kinase inhibitors selected from combinatorial libraries of designed ankyrin repeat proteins. J Biol Chem 280:24715–24722.
-
(2005)
J Biol Chem
, vol.280
, pp. 24715-24722
-
-
Amstutz, P.1
-
24
-
-
71049152941
-
Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: Comparison of DARPin and camelid VHH binding mode
-
Veesler D, et al. (2009) Crystal structure and function of a DARPin neutralizing inhibitor of lactococcal phage TP901-1: Comparison of DARPin and camelid VHH binding mode. J Biol Chem 284:30718–30726.
-
(2009)
J Biol Chem
, vol.284
, pp. 30718-30726
-
-
Veesler, D.1
-
25
-
-
84920848625
-
Designed ankyrin repeat proteins (DARPins): Binding proteins for research, diagnostics, and therapy
-
Plückthun A (2015) Designed ankyrin repeat proteins (DARPins): Binding proteins for research, diagnostics, and therapy. Annu Rev Pharmacol Toxicol 55:489–511.
-
(2015)
Annu Rev Pharmacol Toxicol
, vol.55
, pp. 489-511
-
-
Plückthun, A.1
-
26
-
-
53049085400
-
Chaperone-assisted crystallography with DARPins
-
Sennhauser G, Grütter MG (2008) Chaperone-assisted crystallography with DARPins. Structure 16:1443–1453.
-
(2008)
Structure
, vol.16
, pp. 1443-1453
-
-
Sennhauser, G.1
Grütter, M.G.2
-
27
-
-
85022322918
-
Electron cryo-microscopy structure of the mechanotransduction channel NOMPC
-
Jin P, et al. (2017) Electron cryo-microscopy structure of the mechanotransduction channel NOMPC. Nature 547:118–122.
-
(2017)
Nature
, vol.547
, pp. 118-122
-
-
Jin, P.1
-
28
-
-
77956176007
-
Residue-resolved stability of full-consensus ankyrin repeat proteins probed by NMR
-
Wetzel SK, et al. (2010) Residue-resolved stability of full-consensus ankyrin repeat proteins probed by NMR. J Mol Biol 402:241–258.
-
(2010)
J Mol Biol
, vol.402
, pp. 241-258
-
-
Wetzel, S.K.1
-
29
-
-
78349313323
-
Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module
-
Kramer MA, Wetzel SK, Plückthun A, Mittl PRE, Grütter MG (2010) Structural determinants for improved stability of designed ankyrin repeat proteins with a redesigned C-capping module. J Mol Biol 404:381–391.
-
(2010)
J Mol Biol
, vol.404
, pp. 381-391
-
-
Kramer, M.A.1
Wetzel, S.K.2
Plückthun, A.3
Mittl, P.R.E.4
Grütter, M.G.5
-
30
-
-
84865302440
-
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries
-
Kummer L, et al. (2012) Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries. Proc Natl Acad Sci USA 109:E2248–E2257.
-
(2012)
Proc Natl Acad Sci USA
, vol.109
, pp. E2248-E2257
-
-
Kummer, L.1
-
31
-
-
84864343828
-
A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end
-
Pecqueur L, et al. (2012) A designed ankyrin repeat protein selected to bind to tubulin caps the microtubule plus end. Proc Natl Acad Sci USA 109:12011–12016.
-
(2012)
Proc Natl Acad Sci USA
, vol.109
, pp. 12011-12016
-
-
Pecqueur, L.1
-
32
-
-
84892930656
-
Design, construction, and characterization of a second-generation DARP in library with reduced hydrophobicity
-
Seeger MA, et al. (2013) Design, construction, and characterization of a second-generation DARP in library with reduced hydrophobicity. Protein Sci 22:1239–1257.
-
(2013)
Protein Sci
, vol.22
, pp. 1239-1257
-
-
Seeger, M.A.1
-
33
-
-
84981710085
-
Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein
-
Coscia F, et al. (2016) Fusion to a homo-oligomeric scaffold allows cryo-EM analysis of a small protein. Sci Rep 6:30909.
-
(2016)
Sci Rep
, vol.6
, pp. 30909
-
-
Coscia, F.1
-
34
-
-
84911466782
-
Structure of a designed protein cage that self-assembles into a highly porous cube
-
Lai Y-T, et al. (2014) Structure of a designed protein cage that self-assembles into a highly porous cube. Nat Chem 6:1065–1071.
-
(2014)
Nat Chem
, vol.6
, pp. 1065-1071
-
-
Lai, Y.-T.1
-
35
-
-
84957074708
-
Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression
-
Bale JB, et al. (2015) Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression. Protein Sci 24:1695–1701.
-
(2015)
Protein Sci
, vol.24
, pp. 1695-1701
-
-
Bale, J.B.1
-
36
-
-
51649115555
-
Dynamic charge interactions create surprising rigidity in the ER/K α-helical protein motif
-
Sivaramakrishnan S, Spink BJ, Sim AYL, Doniach S, Spudich JA (2008) Dynamic charge interactions create surprising rigidity in the ER/K α-helical protein motif. Proc Natl Acad Sci USA 105:13356–13361.
-
(2008)
Proc Natl Acad Sci USA
, vol.105
, pp. 13356-13361
-
-
Sivaramakrishnan, S.1
Spink, B.J.2
Sim, A.Y.L.3
Doniach, S.4
Spudich, J.A.5
-
37
-
-
85014129582
-
MotionCor2: Anisotropic correction of beam-induced motion for improved cryo-electron microscopy
-
Zheng SQ, et al. (2017) MotionCor2: Anisotropic correction of beam-induced motion for improved cryo-electron microscopy. Nat Methods 14:331–332.
-
(2017)
Nat Methods
, vol.14
, pp. 331-332
-
-
Zheng, S.Q.1
-
38
-
-
5144235316
-
XMIPP: A new generation of an open-source image processing package for electron microscopy
-
Sorzano CO, et al. (2004) XMIPP: A new generation of an open-source image processing package for electron microscopy. J Struct Biol 148:194–204.
-
(2004)
J Struct Biol
, vol.148
, pp. 194-204
-
-
Sorzano, C.O.1
-
39
-
-
84973518493
-
Processing of structurally heterogeneous cryo-EM data in RELION
-
Scheres SHW (2016) Processing of structurally heterogeneous cryo-EM data in RELION. Methods Enzymol 579:125–157.
-
(2016)
Methods Enzymol
, vol.579
, pp. 125-157
-
-
Scheres, S.H.W.1
-
40
-
-
84894623755
-
Quantifying the local resolution of cryo-EM density maps
-
Kucukelbir A, Sigworth FJ, Tagare HD (2014) Quantifying the local resolution of cryo-EM density maps. Nat Methods 11:63–65.
-
(2014)
Nat Methods
, vol.11
, pp. 63-65
-
-
Kucukelbir, A.1
Sigworth, F.J.2
Tagare, H.D.3
-
41
-
-
4444221565
-
UCSF Chimera–A visualization system for exploratory research and analysis
-
Pettersen EF, et al. (2004) UCSF Chimera–A visualization system for exploratory research and analysis. J Comput Chem 25:1605–1612.
-
(2004)
J Comput Chem
, vol.25
, pp. 1605-1612
-
-
Pettersen, E.F.1
-
43
-
-
84899803729
-
Relaxation of backbone bond geometry improves protein energy landscape modeling
-
Conway P, Tyka MD, DiMaio F, Konerding DE, Baker D (2014) Relaxation of backbone bond geometry improves protein energy landscape modeling. Protein Sci 23:47–55.
-
(2014)
Protein Sci
, vol.23
, pp. 47-55
-
-
Conway, P.1
Tyka, M.D.2
DiMaio, F.3
Konerding, D.E.4
Baker, D.5
-
44
-
-
84996565974
-
Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta
-
Wang RY-R, et al. (2016) Automated structure refinement of macromolecular assemblies from cryo-EM maps using Rosetta. eLife 5:e17219.
-
(2016)
eLife
, vol.5
, pp. e17219
-
-
Wang, R.Y.-R.1
|