2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition

Science

Volumn 351, Issue 6275, 2016, Pages 871-875

  Banerjee, Soojay ^a   Bartesaghi, Alberto ^a   Merk, Alan ^a   Rao, Prashant ^a   Bulfer, Stacie L ^b   Yan, Yongzhao ^c   Green, Neal ^d   Mroczkowski, Barbara ^a   Neitz, R Jeffrey ^b   Wipf, Peter ^c   Falconieri, Veronica ^a   Deshaies, Raymond J ^e   Milne, Jacqueline L S ^a   Huryn, Donna ^c   Arkin, Michelle ^b   Subramaniam, Sriram ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF PITTSBURGH   (United States)

^d FREDERICK NATIONAL LABORATORY FOR CANCER RESEARCH   (United States)

^e California Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE 5' O (3 THIOTRIPHOSPHATE); ADENOSINE DIPHOSPHATE; PROTEIN P97; ADENOSINE 5'-O-(3-THIOTRIPHOSPHATE); ADENOSINE TRIPHOSPHATASE; ADENOSINE TRIPHOSPHATE; ENZYME INHIBITOR; NUCLEAR PROTEIN; P97 ATPASE;

DOMAIN STRUCTURE; DRUG DEVELOPMENT; ENZYME; ENZYME ACTIVITY; INHIBITION; MOLECULAR ANALYSIS; RESOLUTION;

ALLOSTERISM; ARTICLE; CONFORMATIONAL TRANSITION; CRYOELECTRON MICROSCOPY; HUMAN; HYDROGEN BOND; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; X RAY CRYSTALLOGRAPHY; ANALOGS AND DERIVATIVES; ANTAGONISTS AND INHIBITORS; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; PROTEIN TERTIARY STRUCTURE;

ADENOSINE DIPHOSPHATE; ADENOSINE TRIPHOSPHATASES; ADENOSINE TRIPHOSPHATE; ALLOSTERIC REGULATION; BINDING SITES; CRYOELECTRON MICROSCOPY; ENZYME INHIBITORS; HUMANS; MODELS, MOLECULAR; NUCLEAR PROTEINS; PROTEIN STRUCTURE, TERTIARY;

EID: 84958883175     PISSN: 00368075     EISSN: 10959203     Source Type: Journal    
DOI: 10.1126/science.aad7974     Document Type: Article

References (37)

1. P. I. Hanson, S. W. Whiteheart, Nat. Rev. Mol. Cell Biol. 6, 519-529 (2005).
2. T. Ogura, A. J. Wilkinson, Genes Cells 6, 575-597 (2001).
3. D. Barthelme, R. T. Sauer, J. Mol. Biol. 10.1016/j.jmb.2015.11.015 (2015).
4. M. Otter-Nilsson, R. Hendriks, E. I. Pecheur-Huet, D. Hoekstra, T. Nilsson, EMBO J. 18, 2074-2083 (1999).
5. H. Meyer, C. C. Weihl, J. Cell Sci. 127, 3877-3883 (2014).
6. D. Avci, M. K. Lemberg, Trends Cell Biol. 25, 611-622 (2015).
7. L. Fang et al., Front. Cell. Neurosci. 9, 16 (2015).
8. R. J. Deshaies, BMC Biol. 12, 94 (2014).
9. C. W. Valle et al., PLOS ONE 6, e29073 (2011).
10. T. F. Chou et al., Proc. Natl. Acad. Sci. U.S.A. 108, 4834-4839 (2011).
11. T. F. Chou, K. Li, K. J. Frankowski, F. J. Schoenen, R. J. Deshaies, ChemMedChem 8, 297-312 (2013).
12. P. Magnaghi et al., Nat. Chem. Biol. 9, 548-556 (2013).
13. P. Polucci et al., J. Med. Chem. 56, 437-450 (2013).
14. C. Alverez et al., ACS Med. Chem. Lett. 6, 1225-1230 (2015).
15. D. J. Anderson et al., "Inhibition of the AAA-ATPase p97 with the first in class inhibitor CB-5083 as a novel approach to treat cancer." Paper presented at the American Association for Cancer Research annual meeting, Philadelphia, 18 to 22 April 2015.
16. J. M. Davies, A. T. Brünger, W. I. Weis, Structure 16, 715-726 (2008).
17. S. J. Kim et al., J. Biol. Chem. 289, 12264-12274 (2014).
18. P. Hänzelmann, A. Buchberger, H. Schindelin, Structure 19, 833-843 (2011).
19. I. Dreveny et al., EMBO J. 23, 1030-1039 (2004).
20. W. K. Tang, D. Xia, J. Biol. Chem. 288, 36624-36635 (2013).
21. E. Chapman, N. Maksim, F. de la Cruz, J. J. La Clair, Molecules 20, 3027-3049 (2015).
22. C. J. Fang et al., ChemMedChem 10, 52-56 (2015).
23. T. F. Chou, R. J. Deshaies, Autophagy 7, 1091-1092 (2011).
24. S. Tao et al., ACS Chem. Biol. 10, 1916-1924 (2015).
25. P. Yi et al., Mol. Cancer Ther. 11, 2610-2620 (2012).
26. S. J. Brown et al., in Probe Reports from the NIH Molecular Libraries Program (National Center for Biotechnology Information, Bethesda, MD, 2010).
27. M. S. Chimenti et al., J. Biomol. Screen. 20, 788-800 (2015).
28. A. Kucukelbir, F. J. Sigworth, H. D. Tagare, Nat. Methods 11, 63-65 (2014).
29. X. Zhang et al., Mol. Cell 6, 1473-1484 (2000).
30. H. Niwa et al., J. Biol. Chem. 287, 8561-8570 (2012).
31. H. O. Yeung et al., Open Biol 4, 130142 (2014).
32. K. Noi et al., Structure 21, 1992-2002 (2013).
33. W. K. Tang et al., EMBO J. 29, 2217-2229 (2010).
34. W. S. Chia, D. X. Chia, F. Rao, S. Bar Nun, S. Geifman Shochat, PLOS ONE 7, e50490 (2012).
35. F. Beuron et al., EMBO J. 25, 1967-1976 (2006).
36. B. Almeida et al., Biochim. Biophys. Acta 1852, 1950-1959 (2015).
37. A. Bartesaghi et al., Science 348, 1147-1151 (2015).