Purification and Characterization of Latent Polyphenol Oxidase from Apricot (Prunus armeniaca L.)

Journal of Agricultural and Food Chemistry

Volumn 65, Issue 37, 2017, Pages 8203-8212

  Derardja, Ala Eddine ^a,b   Pretzler, Matthias ^a   Kampatsikas, Ioannis ^a   Barkat, Malika ^b   Rompel, Annette ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b Universite Mentouri Constantine   (Algeria)

Author keywords

activation; apricot; characterization; polyphenol oxidase; protein purification; tyrosinase

Indexed keywords

CHARACTERIZATION; CHEMICAL ACTIVATION; MASS SPECTROMETRY;

APRICOT; CHLOROGENIC ACIDS; LOW CONCENTRATIONS; PEPTIDE MASS FINGERPRINTING; POLYPHENOL OXIDASE; PROTEIN PURIFICATION; TEMPERATURE OPTIMUMS; TYROSINASE;

PURIFICATION;

CATECHOL OXIDASE; CHLOROGENIC ACID; PLANT PROTEIN;

APRICOT; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR WEIGHT; PH; TEMPERATURE;

CATECHOL OXIDASE; CHLOROGENIC ACID; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR WEIGHT; PLANT PROTEINS; PRUNUS ARMENIACA; SUBSTRATE SPECIFICITY; TEMPERATURE;

EID: 85029689141     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/acs.jafc.7b03210     Document Type: Article

References (59)

1. Garcia-Viguera, C.; Bridle, P.; Ferreres, F.; Tomas-Barberan, F. A. Influence of variety, maturity and processing on phenolic compounds of apricot juices and jams Z. Lebensm.-Unters. Forsch. 1994, 199, 433-436 10.1007/BF01193268
2. Siddiq, M. Apricots. In Handbook of Fruits and Fruit Processing, 1 st ed..; Hui, Y. H., Ed.; Blackwell Publishing Professional, Ames, IA, 2006; pp 279-290.
3. Whitaker, J. R.; Lee, C. Y. Recent Advances in Chemistry of Enzymatic Browning. In Enzymatic Browning and Its Prevention, Lee, C. Y.; Whitaker, J. R., Eds.; ACS Symposium Series 600, American Chemical Society: Washington, DC, 1995; pp 2-7.
4. Jiménez, A. M.; Martinez-Tome, M.; Egea, I.; Romojaro, F.; Murcia, M. A. Effect of industrial processing and storage on antioxidant activity of apricot (Prunus armeniaca v. bulida) Eur. Food Res. Technol. 2008, 227, 125-134 10.1007/s00217-007-0701-1
5. McEvily, J.; Iyengar, R.; Otwell, W. S. Inhibition of Enzymatic Browning in Foods and Beverages Crit. Rev. Food Sci. Nutr. 1992, 32, 253-273 10.1080/10408399209527599
6. Pretzler, M.; Rompel, A. What causes the different functionality in type-III-copper enzymes? A state of the art perspective Inorg. Chim. Acta 2017, 10.1016/j.ica.2017.04.041
7. Molitor, C.; Mauracher, S. G.; Rompel, A. Aurone synthase is a catechol oxidase with hydroxylase activity and provides insights into the mechanism of plant polyphenol oxidases Proc. Natl. Acad. Sci. U. S. A. 2016, 113, E1806-E1815 10.1073/pnas.1523575113
8. Mayer, A. M. Polyphenol oxidases in plants and fungi: going places? A review Phytochemistry 2006, 67, 2318-2331 10.1016/j.phytochem.2006.08.006
9. Tran, L. T.; Taylor, J. S.; Constabel, C. P. The polyphenol oxidase gene family in land plants: Lineage-specific duplication and expansion BMC Genomics 2012, 13, 395 10.1186/1471-2164-13-395
10. Chevalier, T.; de Rigal, D.; Mbeguie-A-Mbeguie, D.; Gauillard, F.; Richard Forget, F. C.; Fils-Lycaon, B. R. Molecular cloning and characterization of apricot fruit polyphenol oxidase Plant Physiol. 1999, 119, 1261-1270 10.1104/pp.119.4.1261
11. Mauracher, S. G.; Molitor, C.; Al-Oweini, R.; Kortz, U.; Rompel, A. Latent and active abPPO4 mushroom tyrosinase cocrystallized with hexatungstotellurate(VI) in a single crystal Acta Crystallogr., Sect. D: Biol. Crystallogr. 2014, 70, 2301-2315 10.1107/S1399004714013777
12. Pretzler, M.; Bijelic, A.; Rompel, A. Heterologous expression and characterization of functional mushroom tyrosinase (AbPPO4) Sci. Rep. 2017, 7, 1810 10.1038/s41598-017-01813-1
13. Yoruk, R.; Marshall, M. R. Physicochemical properties and function of plant polyphenol oxidase: a review J. Food Biochem. 2003, 27, 361-422 10.1111/j.1745-4514.2003.tb00289.x
14. Solomon, E. I.; Heppner, D. E.; Johnston, E. M.; Ginsbach, J. W.; Cirera, J.; Qayyum, M.; Kieber-Emmons, M. T.; Kjaergaard, C. H.; Hadt, R. G.; Tian, L. Copper active sites in biology Chem. Rev. 2014, 114, 3659-3853 10.1021/cr400327t
15. Cabanes, J.; Escribano, J.; Gandia-Herrero, F.; Garcia-Carmona, F.; Jimenez- Atienzar, M. Partial purification of latent polyphenol oxidase from peach (Prunus persica L. Cv. Catherina), molecular properties and kinetic characterization of soluble and membrane-bound forms J. Agric. Food Chem. 2007, 55, 10446-10451 10.1021/jf072165y
16. Ünal, M. Ü.; Şener, A. Two-year comparison of the biochemical properties of polyphenol oxidase from Turkish Alyanak apricot (Prunus armenica L.) Food Chem. 2016, 190, 741-747 10.1016/j.foodchem.2015.06.025
17. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 1976, 72, 248-254 10.1016/0003-2697(76)90527-3
18. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature 1970, 227, 680-685 10.1038/227680a0
19. Muñoz, J.; García-Molina, F.; Varón, R.; Rodriguez-Lopez, J.; García-Cánovas, F.; Tudela, J. Calculating molar absorptivities for quinones: Application to the measurement of tyrosinase activity Anal. Biochem. 2006, 351, 128-138 10.1016/j.ab.2006.01.011
20. Coseteng, M. Y.; Lee, C. Y. Changes in apple polyphenol oxidase and polyphenol concentrations in relation to degree of browning J. Food Sci. 1987, 52, 985-989 10.1111/j.1365-2621.1987.tb14257.x
21. Galeazzi, M. A. M.; Sgarbieri, V. C.; Constantinides, S. M. Isolation and purification and physicochemical characterization of polyphenoloxidase (PPO) from a dwarf variety of banana (Musa cauendishiil) J. Food Sci. 1981, 46, 150-155 10.1111/j.1365-2621.1981.tb14551.x
22. Golan-Goldhirsh, A.; Whitaker, J. R. Effect of ascorbic acid, sodium bisulfite and thiol compounds on mushroom polyphenol oxidase J. Agric. Food Chem. 1984, 32, 1003-1009 10.1021/jf00125a013
23. Flurkey, W. H.; Jen, J. J. Purification of peach polyphenol oxidase in the presence of added protease inhibitors J. Food Biochem. 1980, 4, 29-41 10.1111/j.1745-4514.1980.tb00875.x
24. Fraignier, M.; Marques, L.; Fleuriet, A.; Macheix, J. Biochemical and immunochemical characterisation of polyphenol oxidase from different prunus fruits J. Agric. Food Chem. 1995, 43, 2375-2380 10.1021/jf00057a011
25. Arslan, O.; Temur, A.; Tozlu, I. Polyphenol oxidase from Malatya apricot J. Agric. Food Chem. 1998, 46, 1239-1241 10.1021/jf970599v
26. Yemenicioǧlu, A.; Cemeroǧlu, B. Consistency of Polyphenol Oxidase (PPO) Thermostability in Ripening Apricots (Prunus armeniaca L.): Evidence for the Presence of Thermostable PPO Forming and Destabilizing Mechanisms in Apricots J. Agric. Food Chem. 2003, 51, 2371-2379 10.1021/jf025988q
27. Liu, L.; Cao, S.; Xie, B.; Sun, Z.; Li, X.; Miao, W. Characterization of polyphenol oxidase from litchi pericarp using (-)-epicatechin as substrate J. Agric. Food Chem. 2007, 55, 7140-7143 10.1021/jf070964a
28. Nagai, T.; Suzuki, N. Partial purification of polyphenol oxidase from Chinese cabbage Brassica rapa L J. Agric. Food Chem. 2001, 49, 3922-3926 10.1021/jf000694v
29. Aydin, B.; Gulcin, I.; Alwasel, S. H. Purification and Characterization of Polyphenol Oxidase from Hemşin Apple (Malus communis L.) Int. J. Food Prop. 2015, 18, 2735-2745 10.1080/10942912.2015.1012725
30. Siddiq, M.; Sinha, N. K.; Cash, J. N.; Hanum, T. Partial purification of polyphenol oxidase from plums (Prunus dornesfica L., cv. Stanley) J. Food Biochem. 1996, 20, 111-123 10.1111/j.1745-4514.1996.tb00576.x
31. Siddiq, M.; Dolan, K. D. Characterization of polyphenol oxidase from blueberry (Vaccinium corymbosum L.) Food Chem. 2017, 218, 216-220 10.1016/j.foodchem.2016.09.061
32. Serradell, M. A.; Rozenfeld, P. A.; Martinez, G. A.; Civello, P. M.; Chaves, A. R.; Añón, M. C. Polyphenoloxidase activity from strawberry fruit (Fragaria x ananassa, Duch., cv Selva): characterization and partial purification J. Sci. Food Agric. 2000, 80, 1421-1427 10.1002/1097-0010(200007)80:9<1421::AID-JSFA649>3.3.CO;2-B
33. Dincer, B.; Colak, A.; Aydin, N.; Kadioglu, A.; Guner, S. Characterization of polyphenoloxidase from medlar fruits (Mespilus germanica L., Rosaceae) Food Chem. 2002, 77, 1-7 10.1016/S0308-8146(01)00359-4
34. Duangmal, K.; Owusu Apenten, R. K. A comparative study of polyphenoloxidases from taro (Colocasia esculenta) and potato (Solanum tuberosum var. Romano) Food Chem. 1999, 64, 351-359 10.1016/S0308-8146(98)00127-7
35. Palma-Orozco, G.; Ortiz-Moreno, A.; Dorantes-Alvarez, L.; Sampedro, J. G.; Najera, H. Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota) Phytochemistry 2011, 72, 82-88 10.1016/j.phytochem.2010.10.011
36. Alici, E. H.; Arabaci, G. Purification of polyphenol oxidase from borage (Trachystemon orientalis L.) by using three-phase partitioning and investigation of kinetic properties Int. J. Biol. Macromol. 2016, 93, 1051-1056 10.1016/j.ijbiomac.2016.09.070
37. Mazzafera, P.; Robinson, S. P. Characterisation of polyphenol oxidase in coffee Phytochemistry 2000, 55, 285-296 10.1016/S0031-9422(00)00332-0
38. Marrufo-Hernández, N. A.; Palma-Orozco, G.; Beltrán, H. I.; Nájera, H. Purification, partial biochemical characterization and inactivation of polyphenol oxidase from Mexican Golden Delicious apple (Malus domestica) J. Food Biochem. 2017, 41, e12356 10.1111/jfbc.12356
39. Dragovic-Uzelac, V.; Levaj, B.; Mrkic, V.; Bursac, D.; Boras, M. The content of polyphenols and carotenoids in three apricot cultivars depending on stage of maturity and geographical region Food Chem. 2007, 102, 966-975 10.1016/j.foodchem.2006.04.001
40. Dijkstra, L.; Walker, J. R. L. Enzymic Browning in Apricots J. Sci. Food Agric. 1991, 54, 229-234 10.1002/jsfa.2740540208
41. Palma-Orozco, G.; Marrufo-Hernández, N. A.; Sampedro, J. G.; Nájera, H. Purification and Partial Biochemical Characterization of Polyphenol Oxidase from Mango (Mangifera indica cv. Manila) J. Agric. Food Chem. 2014, 62, 9832-9840 10.1021/jf5029784
42. Zhou, H. W.; Feng, X. Polyphenol Oxidase from Yali Pear (Pyrus bretschneideri) J. Sci. Food Agric. 1991, 57, 307-313 10.1002/jsfa.2740570302
43. Siddiq, M.; Cash, J. N.; Sinha, N. K.; Akhter, P. Characterization and inhibition of PPO from pears (Pyrus communis L. ev Bose and Red) J. Food Biochem. 1994, 17, 327-337 10.1111/j.1745-4514.1993.tb00477.x
44. Ünal, M. Ü.; Şener, A. Determination of some biochemical properties of polyphenol oxidase from Emir grape (Vitis vinifera L. cv. Emir) J. Sci. Food Agric. 2006, 86, 2374-2379 10.1002/jsfa.2627
45. Yue-Ming, J.; Zauberman, G.; Fuchs, Y. Partial purification and some properties of polyphenol oxidase extracted from litchi fruit pericarp Postharvest Biol. Biotechnol. 1997, 10, 221-228 10.1016/j.foodchem.2012.10.103
46. Jiang, Y. M. Purification and some properties of longan fruit Food Chem. 1999, 66, 75-79
47. Liu, N. N.; Liu, W.; Wang, D. J.; Zhou, Y. B.; Lin, X. J.; Wang, X.; Li, S. B. Purification and partial characterization of polyphenol oxidase from the flower buds of Lonicera japonica Thunb Food Chem. 2013, 138, 478-483
48. Sun, H. J.; Wang, J.; Tao, X. M.; Shi, J.; Huang, M. Y.; Chen, Z. Purification and Characterization of Polyphenol Oxidase from Rape Flower J. Agric. Food Chem. 2012, 60, 823-829 10.1021/jf2032999
49. Mayer, A. M. Polyphenol oxidases in plants-recent progress Phytochemistry 1987, 26, 11-20 10.1016/S0031-9422(00)81472-7
50. Sojo, M. M.; Núñez-Delicado, E.; García-Carmona, F.; Sánchez- Ferrer, A. Partial purification of a banana polyphenol oxidase using triton X-114 and PEG 8000 for removal of polyphenols J. Agric. Food Chem. 1998, 46, 4924-4930 10.1021/jf980473d
51. Mauracher, S. G.; Molitor, C.; Michael, C.; Kragl, M.; Rizzi, A.; Rompel, A. High level protein-purification allows the unambiguous polypeptide determination of latent isoform PPO4 of mushroom tyrosinase Phytochemistry 2014, 99, 14-25 10.1016/j.phytochem.2013.12.016
52. Molitor, C.; Mauracher, S. G.; Pargan, S.; Mayer, R. L.; Halbwirth, H.; Rompel, A. Latent and active aurone synthase from petals of C. grandiflora: a polyphenol oxidase with unique characteristics Planta 2015, 242, 519-537 10.1007/s00425-015-2261-0
53. Moore, B. M.; Flurkey, W. H. Sodium dodecyl sulfate activation of a plant polyphenoloxidase J. Biol. Chem. 1990, 265, 4982-4988
54. Das, J. R.; Bhat, S. G.; Gowda, L. R. Purification and characterization of a polyphenol oxidase from the kew cultivar of Indian pineapple fruit J. Agric. Food Chem. 1997, 45, 2031-2035 10.1021/jf9607674
55. Paul, B.; Gowda, L. R. Purification and Characterization of a Polyphenol Oxidase from the Seeds of Field Bean (Dolichos lablab) J. Agric. Food Chem. 2000, 48, 3839-3846 10.1021/jf000296s
56. Raffert, G.; Flurkey, W. H. Carbohydrate associated with broad bean polyphenol oxidase is resistant to enzymatic and chemical deglycosylation Phytochemistry 1995, 38, 1355-1360 10.1016/0031-9422(94)00816-C
57. Marusek, C. M.; Trobaugh, N. M.; Flurkey, W. H.; Inlow, J. K. Comparative analysis of polyphenol oxidase from plant and fungal species J. Inorg. Biochem. 2006, 100, 108-123 10.1016/j.jinorgbio.2005.10.008
58. Lerch, K. Primary structure of tyrosinase from Neurospora crassa. II. Complete amino acid sequence and chemical structure of a tripeptide containing an unusual thioether J. Biol. Chem. 1982, 257, 6414-6419
59. Zekiri, F.; Molitor, C.; Mauracher, S. G.; Michael, C.; Mayer, R. L.; Gerner, C.; Rompel, A. Purification and characterization of tyrosinase from walnut leaves (Juglans regia) Phytochemistry 2014, 101, 5-15 10.1016/j.phytochem.2014.02.010