Purification and characterization of polyphenol oxidase from rape flower

Journal of Agricultural and Food Chemistry

Volumn 60, Issue 3, 2012, Pages 823-829

  Sun, Han Ju ^a   Wang, Jing ^a   Tao, Xue Ming ^a   Shi, Juan ^a   Huang, Mei Ying ^a   Chen, Zhe ^a  

^a HEFEI UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

catechol; characterization; Polyphenol oxidase (PPO); purification; rape flower

Indexed keywords

, INHIBITOR; AMMONIUM SULFATE; ASCORBIC ACIDS; CATECHOL; CRUDE ENZYMES; ETHYLENE DIAMINE TETRA-ACETIC ACID; GEL CHROMATOGRAPHY; INHIBITIVE EFFECTS; MICHAELIS-MENTEN CONSTANT; OPTIMAL CONDITIONS; PH VALUE; POLYPHENOL OXIDASE; PRELIMINARY TREATMENT; PURIFICATION FACTORS; RAPE FLOWER; REACTION VELOCITIES; SEPHADEX; SODIUM SULFITE;

AMMONIUM COMPOUNDS; CALCIUM CHLORIDE; CHARACTERIZATION; CITRIC ACID; DIALYSIS; DIMERS; ENZYMES; ION EXCHANGE; KETONES; MOLECULAR WEIGHT; OPTIMIZATION; PHENOLS; SODIUM; SODIUM CHLORIDE;

PURIFICATION;

CATECHOL OXIDASE; VEGETABLE PROTEIN;

ARTICLE; BRASSICA RAPA; CHEMISTRY; DIMERIZATION; ENZYME STABILITY; ENZYMOLOGY; FLOWER; ISOLATION AND PURIFICATION; KINETICS; MOLECULAR WEIGHT; PH;

BRASSICA RAPA; CATECHOL OXIDASE; DIMERIZATION; ENZYME STABILITY; FLOWERS; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR WEIGHT; PLANT PROTEINS;

EID: 84863016586     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf2032999     Document Type: Article

References (32)

1. Sun, H.-J.; Jiang, S.-T.; Zi, M.-Y.; Ding, Q. Purification, chemical composition and in vitro antioxidant activity of two protein-bound polysaccharides from rapeseed meal Food Sci. Biotechnol. 2009, 18, 1386-1391
2. Sun, H.-J.; Jiang, S.-T.; Pan, M.; Ding, Q. In vivo antioxidative capacities of rapeseed meal polysaccharides J. Food Agric. Environ. 2009, 7, 97-102
3. Sun, H.-J.; Ding, Q.; Xu, J.-Y.; Tong, J.; Shi, J.; Chen, Z. Fractionation of polysaccharides from rapeseed by ultrafiltration: effect of molecular pore size and operation conditions on the membrane performance Sep. Purif. Technol. 2011, 80, 670-676
4. Espin, J. C.; Morales, M.; Varon, R.; Tudela, J.; Garcia-Canovas, F. Monophenolase activity of polyphenol oxidase from Verdedoncella apple J. Agric. Food Chem. 1995, 43, 2807-2812
5. Galleazi, M. A.; Sgarbieri, V. C.; Constantinides, S. M. Isolation, purification and physicochemical characterization of PPO from a dwarf variety of banana (Musa cavensdishii L.) J. Food Sci. 1981, 46, 150-155
6. Chen, J.-S.; Preston, J. F.; Wei, C.-I.; Hooshar, P.; Gleeson, R. A.; Marshall, M. R. Structural comparison of crustacean, potato, and mushroom polyphenol oxidases J. Agric. Food Chem. 1992, 40, 1326-1330
7. Paul, B.; Gowda, L. R. Purification and characterization of a polyphenol oxidase from the seeds of field bean (Dolichos lablab) J. Agric. Food Chem. 2000, 48, 3839-3846
8. Fujita, S.; Saari, N. B.; Maegawa, M.; Tetsuka, T.; Hayashi, N.; Tono, T. Purification and properties of polyphenol oxidase from cabbage (Brassica oleracea L.) J. Agric. Food Chem. 1995, 43, 1138-1142
9. Zou, W.; Chen, Y.; Lu, C. Differences in biochemical responses to cold stress in two contrasting varieties of rape seed (Brassica napus L.) For. Stud. China 2007, 9, 142-146
10. Kumar, V. B. A.; Mohan, T. C. K.; Murugan, K. Purification and kinetic characterization of polyphenol oxidase from Barbados cherry (Malpighia glabra L.) Food Chem. 2008, 110, 328-333
11. Palma-Orozco, G.; Ortiz-Moreno, A.; Dorantes-álvarez, L.; Sampedro, J. G.; Nájera, H. Purification and partial biochemical characterization of polyphenol oxidase from mamey (Pouteria sapota) Phytochemistry 2011, 72, 82-88
12. Das, J. R.; Bhat, S. G.; Gowda, L. R. Purification and characterization of a polyphenol oxidase from the kew cultivar of Indian pineapple fruit J. Agric. Food Chem. 1997, 45, 2031-2035 (Pubitemid 127485519)
13. Flurkey, W. H.; Joseph, J. J. Hydrophobic adsorption chromatography of peach polyphenol oxidase J. Food Sci. 1980, 45, 1622-1624
14. Ayaz, F. A.; Demir, O.; Torun, H.; Kolcuoglu, Y.; Colak, A. Characterization of polyphenoloxidase (PPO) and total phenolic contents in medlar (Mespilus germanica L.) fruit during ripening and over ripening Food Chem. 2008, 106, 291-298 (Pubitemid 47332119)
15. Lineweaver, H.; Burk, D. The determination of enzyme dissociation constant J. Am. Chem. Soc. 1934, 56, 658-666
16. Bradford, M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal. Biochem. 1976, 72, 248-254
17. Constantinides, S. M.; Bedford, C. L. Multiple forms of phenoloxidase J. Food Sci. 1967, 32, 446-450
18. Rivas, N. D. J.; Whitaker, J. R. Purification and some properties of two polyphenol oxidases from Bartlett pears Plant Physiol. 1973, 52, 501-507
19. Kahn, V. Polyphenol oxidase isoenzymes in avocado Phytochemistry 1976, 15, 267-272
20. Gómez-López, V. M. Some biochemical properties of polyphenol oxidase from two varieties of avocado Food Chem. 2002, 77, 163-169 (Pubitemid 34518230)
21. Dincer, B.; Colak, A.; Aydin, N.; Kadioglu, A.; Güner, S. Characterization of polyphenoloxidase from medlar fruits (Mespilus germanica L., Rosaceae) Food Chem. 2002, 77, 1-7 (Pubitemid 34230379)
22. Wesche-Ebeling, P.; Montgomery, M. W. Strawberry polyphenoloxidase: purification and characterization J. Food Sci. 1990, 55, 1315-1319
23. Gregory, R. P. F.; Bendall, D. S. The purification and some properties of polyphenol oxidase from tea (Camellia sinensis L.) Biochem. J. 1966, 101, 569-581
24. Ziyan, E.; Perkyardimci, S. Purification and characterization of pear (Pyrus communis) polyphenol oxidase Turk. J. Chem. 2004, 28, 547-557 (Pubitemid 39586174)
25. Siddiq, M.; Sinha, N. K.; Cash, J. N. Characterization of polyphenoloxidase from Stanley plums J. Food Sci. 1992, 57, 1177-1179
26. Mayer, A. M. Polyphenol oxidases in plants and fungi: going places? A review Phytochemistry 2006, 67, 2318-2331 (Pubitemid 44527933)
27. Perera, E.; Moyano, F. J.; Díaz, M.; Perdomo-Morales, R.; Montero-Alejo, V.; Alonso, E.; Carrillo, O.; Galich, G. S. Polymorphism and partial characterization of digestive enzymes in the spiny lobster Panulirus argus Comp. Biochem. Physiol., Part B: Biochem. Mol. Biol. 2008, 150, 247-254
28. Fernandez-Lafuente, R.; Guisan, J. M.; Ali, S.; Cowan, D. Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability Enzyme Microb. Technol. 2000, 26, 568-573 (Pubitemid 30227645)
29. Nagai, T.; Suzuki, N. Partial purification of polyphenol oxidase from Chinese cabbage Brassica rapa L J. Agric. Food Chem. 2001, 49, 3922-3926 (Pubitemid 32822717)
30. Mazzafera, P.; Robinson, S. P. Characterization of polyphenol oxidase in coffee Phytochemistry 2000, 55, 285-296
31. Weemaes, C.; Ludikhuyze, L.; Broeck, I. V. D.; Hendrickx, M. High pressure inactivation of polyphenoloxidase J. Food Sci. 1998, 63, 873-877
32. Goulart, P. D. F. P.; Alves, J. D.; Magalhães, M. M.; Lima, L. C. D. O.; Meyer, L. E. Purification of polyphenoloxidase from coffee fruits Food Chem. 2003, 83, 7-11 (Pubitemid 36945449)