Multiplexed Engineering and Analysis of Combinatorial Enhancer Activity in Single Cells

Molecular Cell

Volumn 66, Issue 2, 2017, Pages 285-299.e5

  Xie, Shiqi ^a   Duan, Jialei ^a   Li, Boxun ^a   Zhou, Pei ^a   Hon, Gary C ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

CRISPR; enhancers; epigenetics; gene editing; gene regulation; single cell RNA seq

Indexed keywords

DEOXYRIBONUCLEASE I; E1A ASSOCIATED P300 PROTEIN; HEMOGLOBIN BETA CHAIN; PROTEIN; PROTEIN KINASE PIM 1; PUROMYCIN; RNAPII PROTEIN; TRANSCRIPTOME; TUMOR NECROSIS FACTOR; UNCLASSIFIED DRUG; GUIDE RNA; HISTONE ACETYLTRANSFERASE PCAF; RNA POLYMERASE II; TN5 TRANSPOSASE; TRANSCRIPTION FACTOR; TRANSPOSASE;

ARTICLE; CHROMATIN; CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT; CONCEPTUAL FRAMEWORK; CONTROLLED STUDY; ENHANCER REGION; EPIGENETICS; FEASIBILITY STUDY; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENE LOCUS; GENE REPRESSION; GENETIC ENGINEERING; GENOTYPE; GOLD STANDARD; HISTONE ACETYLATION; HISTONE MODIFICATION; HUMAN; HUMAN CELL; LEUKEMIA CELL LINE; MOSAIC SINGLE CELL ANALYSIS BY INDEXED CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEAT SEQUENCING; NONHUMAN; PENETRANCE; POLYMERASE CHAIN REACTION; PROMOTER REGION; QUANTITATIVE ANALYSIS; RNA SEQUENCE; SINGLE CELL ANALYSIS; CELL SEPARATION; CRISPR CAS SYSTEM; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENETIC DATABASE; GENETIC TRANSCRIPTION; GENETIC TRANSFECTION; GENETICS; HEK293 CELL LINE; HIGH THROUGHPUT SEQUENCING; K-562 CELL LINE; METABOLISM; PHENOTYPE; PROCEDURES; TRANSCRIPTION INITIATION;

CELL SEPARATION; CRISPR-CAS SYSTEMS; DATABASES, GENETIC; ENHANCER ELEMENTS, GENETIC; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; GENOTYPE; HEK293 CELLS; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; HUMANS; K562 CELLS; P300-CBP TRANSCRIPTION FACTORS; PENETRANCE; PHENOTYPE; RNA POLYMERASE II; RNA, GUIDE; SINGLE-CELL ANALYSIS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC; TRANSCRIPTIONAL ACTIVATION; TRANSCRIPTOME; TRANSFECTION; TRANSPOSASES;

EID: 85017425829     PISSN: 10972765     EISSN: 10974164     Source Type: Journal    
DOI: 10.1016/j.molcel.2017.03.007     Document Type: Article

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