Lipid-mediated Wnt protein stabilization enables serum-free culture of human organ stem cells

Nature Communications

Volumn 8, Issue , 2017, Pages

  Tuÿsüz, Nesrin ^a   Van Bloois, Louis ^b   Van Den Brink, Stieneke ^c,d   Begthel, Harry ^c,d   Verstegen, Monique M A ^a   Cruz, Luis J ^e   Hui, Lijian ^f   Van Der Laan, Luc J W ^a   De Jonge, Jeroen ^a   Vries, Robert ^c,d   Braakman, Eric ^a   Mastrobattista, Enrico ^b   Cornelissen, Jan J ^a   Clevers, Hans ^c,d   Ten Berge, Derk ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

^b UTRECHT UNIVERSITY   (Netherlands)

^c UNIVERSITY MEDICAL CENTER UTRECHT   (Netherlands)

^d Foundation Hubrecht Organoid Technology (HUB)   (Netherlands)

^e LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^f INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; CHOLESTEROL; LIPID; LIPOSOME; PHOSPHOLIPID; WNT3A PROTEIN; WNT3A PROTEIN, HUMAN;

CELLS AND CELL COMPONENTS; CHEMICAL COMPOUND; CONCENTRATION (COMPOSITION); HYDROPHOBICITY; PHOSPHOLIPID; PROTEIN; SERUM; SOLUBILIZATION; STABILIZATION;

ADULT; ANIMAL CELL; ARTICLE; CELL DIFFERENTIATION; CONDITIONED MEDIUM; EMBRYO; EMBRYONIC STEM CELL; ENDOCRINE CELL; ENTEROENDOCRINE CELL; EXPRESSION VECTOR; GENE ACTIVATION; HEK293 CELL LINE; HUMAN; HUMAN CELL; HUMAN TISSUE; INTESTINE BIOPSY; INTESTINE CELL; LIPOPHILICITY; MOUSE; MULTIPOTENT STEM CELL; NONHUMAN; PROTEIN PURIFICATION; PROTEIN STABILITY; SERUM-FREE MEDIUM; SOLUBILIZATION; STEM CELL CULTURE; STEM CELL EXPANSION; STEM CELL SELF-RENEWAL; WNT SIGNALING PATHWAY; ADULT STEM CELL; BIOPSY; CHEMICAL PHENOMENA; CHEMISTRY; DRUG EFFECT; END STAGE LIVER DISEASE; HEPATITIS C; JEJUNUM; LIVER; LIVER CELL CARCINOMA; LIVER TUMOR; METABOLISM; ORGANOID; PATHOLOGY; PHARMACOLOGY; TISSUE CULTURE TECHNIQUE; WILSON DISEASE;

ADULT STEM CELLS; BIOPSY; CARCINOMA, HEPATOCELLULAR; CHOLESTEROL; CULTURE MEDIA, CONDITIONED; END STAGE LIVER DISEASE; HEPATITIS C; HEPATOLENTICULAR DEGENERATION; HUMANS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; JEJUNUM; LIPOSOMES; LIVER; LIVER NEOPLASMS; ORGANOIDS; PHOSPHOLIPIDS; TISSUE CULTURE TECHNIQUES; WNT3A PROTEIN;

EID: 85014683151     PISSN: None     EISSN: 20411723     Source Type: Journal    
DOI: 10.1038/ncomms14578     Document Type: Article

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