-
1
-
-
85045290824
-
Recent advances in biosensor technology for potential applications—an overview
-
1 Vigneshvar, S., Sudhakumari, C., Senthilkumaran, B., Prakash, H., Recent advances in biosensor technology for potential applications—an overview. Front Bioeng Biotechnol, 2016, 4.
-
(2016)
Front Bioeng Biotechnol
, pp. 4
-
-
Vigneshvar, S.1
Sudhakumari, C.2
Senthilkumaran, B.3
Prakash, H.4
-
2
-
-
0021997716
-
Genetically-manipulated microorganisms and their products in the oil service industries
-
2 Chakrabarty, A.M., Genetically-manipulated microorganisms and their products in the oil service industries. Trends Biotechnol 3 (1985), 32–39.
-
(1985)
Trends Biotechnol
, vol.3
, pp. 32-39
-
-
Chakrabarty, A.M.1
-
3
-
-
85005991783
-
Cell-free synthetic biology: engineering beyond the cell
-
3 Perez, J.G., Stark, J.C., Jewett, M.C., Cell-free synthetic biology: engineering beyond the cell. Cold Spring Harbor Perspect Biol, 2016, 2016, a023853.
-
(2016)
Cold Spring Harbor Perspect Biol
, vol.2016
, pp. a023853
-
-
Perez, J.G.1
Stark, J.C.2
Jewett, M.C.3
-
4
-
-
84906213344
-
The emerging age of cell-free synthetic biology
-
4 Smith, M.T., Wilding, K.M., Hunt, J.M., Bennett, A.M., Bundy, B.C., The emerging age of cell-free synthetic biology. FEBS Lett 588 (2014), 2755–2761.
-
(2014)
FEBS Lett
, vol.588
, pp. 2755-2761
-
-
Smith, M.T.1
Wilding, K.M.2
Hunt, J.M.3
Bennett, A.M.4
Bundy, B.C.5
-
5
-
-
2942541593
-
A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter
-
5 Pellinen, T., Huovinen, T., Karp, M., A cell-free biosensor for the detection of transcriptional inducers using firefly luciferase as a reporter. Anal Biochem 330 (2004), 52–57.
-
(2004)
Anal Biochem
, vol.330
, pp. 52-57
-
-
Pellinen, T.1
Huovinen, T.2
Karp, M.3
-
6
-
-
84864953230
-
Cell-free protein synthesis: applications come of age
-
6 Carlson, E.D., Gan, R., Hodgman, C.E., Jewett, M.C., Cell-free protein synthesis: applications come of age. Biotechnol Adv 30 (2012), 1185–1194.
-
(2012)
Biotechnol Adv
, vol.30
, pp. 1185-1194
-
-
Carlson, E.D.1
Gan, R.2
Hodgman, C.E.3
Jewett, M.C.4
-
7
-
-
79956158054
-
Microscale to manufacturing scale-up of cell-free cytokine production—a new approach for shortening protein production development timelines
-
7 Zawada, J.F., Yin, G., Steiner, A.R., Yang, J., Naresh, A., Roy, S.M., Gold, D.S., Heinsohn, H.G., Murray, C.J., Microscale to manufacturing scale-up of cell-free cytokine production—a new approach for shortening protein production development timelines. Biotechnol Bioeng 108 (2011), 1570–1578.
-
(2011)
Biotechnol Bioeng
, vol.108
, pp. 1570-1578
-
-
Zawada, J.F.1
Yin, G.2
Steiner, A.R.3
Yang, J.4
Naresh, A.5
Roy, S.M.6
Gold, D.S.7
Heinsohn, H.G.8
Murray, C.J.9
-
8
-
-
84956609810
-
Engineering an allosteric transcription factor to respond to new ligands
-
8 Taylor, N.D., Garruss, A.S., Moretti, R., Chan, S., Arbing, M.A., Cascio, D., Rogers, J.K., Isaacs, F.J., Kosuri, S., Baker, D., Engineering an allosteric transcription factor to respond to new ligands. Nat Methods 13 (2016), 177–183.
-
(2016)
Nat Methods
, vol.13
, pp. 177-183
-
-
Taylor, N.D.1
Garruss, A.S.2
Moretti, R.3
Chan, S.4
Arbing, M.A.5
Cascio, D.6
Rogers, J.K.7
Isaacs, F.J.8
Kosuri, S.9
Baker, D.10
-
9
-
-
84992709449
-
Selection and biosensor application of aptamers for small molecules
-
9 Mayer, G., Pfeiffer, F., Selection and biosensor application of aptamers for small molecules. Front Chem, 4, 2016, 25.
-
(2016)
Front Chem
, vol.4
, pp. 25
-
-
Mayer, G.1
Pfeiffer, F.2
-
10
-
-
79956224136
-
Model for biological communication in a nanofabricated cell-mimic driven by stochastic resonance
-
10 Karig, D.K., Siuti, P., Dar, R.D., Retterer, S.T., Doktycz, M.J., Simpson, M.L., Model for biological communication in a nanofabricated cell-mimic driven by stochastic resonance. Nano Commun Netw 2 (2011), 39–49.
-
(2011)
Nano Commun Netw
, vol.2
, pp. 39-49
-
-
Karig, D.K.1
Siuti, P.2
Dar, R.D.3
Retterer, S.T.4
Doktycz, M.J.5
Simpson, M.L.6
-
11
-
-
45749152670
-
Rapid screening of quorum-sensing signal N-acyl homoserine lactones by an in vitro cell-free assay
-
11 Kawaguchi, T., Chen, Y.P., Norman, R.S., Decho, A.W., Rapid screening of quorum-sensing signal N-acyl homoserine lactones by an in vitro cell-free assay. Appl Environ Microbiol 74 (2008), 3667–3671.
-
(2008)
Appl Environ Microbiol
, vol.74
, pp. 3667-3671
-
-
Kawaguchi, T.1
Chen, Y.P.2
Norman, R.S.3
Decho, A.W.4
-
12
-
-
85015135135
-
Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets
-
Cell-free reagents in water-in-oil emulsions were used to sense and report as well as to produce a bacterial quorum sensing signal. This shows the potential of cell-free synthetic biology for both sensing and interacting with the environment.
-
12• Schwarz-Schilling, M., Aufinger, L., Mückl, A., Simmel, F., Chemical communication between bacteria and cell-free gene expression systems within linear chains of emulsion droplets. Integr Biol 8 (2016), 564–570 Cell-free reagents in water-in-oil emulsions were used to sense and report as well as to produce a bacterial quorum sensing signal. This shows the potential of cell-free synthetic biology for both sensing and interacting with the environment.
-
(2016)
Integr Biol
, vol.8
, pp. 564-570
-
-
Schwarz-Schilling, M.1
Aufinger, L.2
Mückl, A.3
Simmel, F.4
-
13
-
-
85013220725
-
Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology
-
gkt052
-
13 Chappell, J., Jensen, K., Freemont, P.S., Validation of an entirely in vitro approach for rapid prototyping of DNA regulatory elements for synthetic biology. Nucleic Acids Res, 2013, 2016 gkt052.
-
(2016)
Nucleic Acids Res
, vol.2013
-
-
Chappell, J.1
Jensen, K.2
Freemont, P.S.3
-
14
-
-
84860375823
-
Expression optimization and synthetic gene networks in cell-free systems
-
14 Karig, D.K., Iyer, S., Simpson, M.L., Doktycz, M.J., Expression optimization and synthetic gene networks in cell-free systems. Nucleic Acids Res 40 (2012), 3763–3774.
-
(2012)
Nucleic Acids Res
, vol.40
, pp. 3763-3774
-
-
Karig, D.K.1
Iyer, S.2
Simpson, M.L.3
Doktycz, M.J.4
-
15
-
-
84927717726
-
Aptamer selection technology and recent advances
-
15 Blind, M., Blank, M., Aptamer selection technology and recent advances. Mol Ther—Nucleic Acids, 4, 2015, e223.
-
(2015)
Mol Ther—Nucleic Acids
, vol.4
, pp. e223
-
-
Blind, M.1
Blank, M.2
-
16
-
-
84948757225
-
Current approaches in SELEX: an update to aptamer selection technology
-
16 Darmostuk, M., Rimpelova, S., Gbelcova, H., Ruml, T., Current approaches in SELEX: an update to aptamer selection technology. Biotechnol Adv 33 (2015), 1141–1161.
-
(2015)
Biotechnol Adv
, vol.33
, pp. 1141-1161
-
-
Darmostuk, M.1
Rimpelova, S.2
Gbelcova, H.3
Ruml, T.4
-
17
-
-
84903178620
-
Thrombin-mediated transcriptional regulation using DNA aptamers in DNA-based cell-free protein synthesis
-
17 Iyer, S., Doktycz, M.J., Thrombin-mediated transcriptional regulation using DNA aptamers in DNA-based cell-free protein synthesis. ACS Synth Biol 3 (2013), 340–346.
-
(2013)
ACS Synth Biol
, vol.3
, pp. 340-346
-
-
Iyer, S.1
Doktycz, M.J.2
-
18
-
-
79951519285
-
Rational design of artificial riboswitches based on ligand-dependent modulation of internal ribosome entry in wheat germ extract and their applications as label-free biosensors
-
18 Ogawa, A., Rational design of artificial riboswitches based on ligand-dependent modulation of internal ribosome entry in wheat germ extract and their applications as label-free biosensors. RNA 17 (2011), 478–488.
-
(2011)
RNA
, vol.17
, pp. 478-488
-
-
Ogawa, A.1
-
19
-
-
84909963314
-
Toehold switches: de-novo-designed regulators of gene expression
-
19 Green, A.A., Silver, P.A., Collins, J.J., Yin, P., Toehold switches: de-novo-designed regulators of gene expression. Cell 159 (2014), 925–939.
-
(2014)
Cell
, vol.159
, pp. 925-939
-
-
Green, A.A.1
Silver, P.A.2
Collins, J.J.3
Yin, P.4
-
20
-
-
84909963313
-
Paper-based synthetic gene networks
-
20 Pardee, K., Green, A.A., Ferrante, T., Cameron, D.E., DaleyKeyser, A., Yin, P., Collins, J.J., Paper-based synthetic gene networks. Cell 159 (2014), 940–954.
-
(2014)
Cell
, vol.159
, pp. 940-954
-
-
Pardee, K.1
Green, A.A.2
Ferrante, T.3
Cameron, D.E.4
DaleyKeyser, A.5
Yin, P.6
Collins, J.J.7
-
21
-
-
84973486576
-
Rapid, low-cost detection of Zika virus using programmable biomolecular components
-
21 Pardee, K., Green Alexander, A., Takahashi Melissa, K., Braff, D., Lambert, G., Lee Jeong, W., Ferrante, T., Ma, D., Donghia, N., Fan, M., et al. Rapid, low-cost detection of Zika virus using programmable biomolecular components. Cell 165 (2016), 1255–1266.
-
(2016)
Cell
, vol.165
, pp. 1255-1266
-
-
Pardee, K.1
Green Alexander, A.2
Takahashi Melissa, K.3
Braff, D.4
Lambert, G.5
Lee Jeong, W.6
Ferrante, T.7
Ma, D.8
Donghia, N.9
Fan, M.10
-
22
-
-
41549119008
-
An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum
-
22 Ferapontova, E.E., Olsen, E.M., Gothelf, K.V., An RNA aptamer-based electrochemical biosensor for detection of theophylline in serum. J Am Chem Soc 130 (2008), 4256–4258.
-
(2008)
J Am Chem Soc
, vol.130
, pp. 4256-4258
-
-
Ferapontova, E.E.1
Olsen, E.M.2
Gothelf, K.V.3
-
23
-
-
84961223490
-
Cell-free synthesis of a functional membrane transporter into a tethered bilayer lipid membrane
-
23 Zieleniecki, J.L., Nagarajan, Y., Waters, S., Rongala, J., Thompson, V., Hrmova, M., Köper, I., Cell-free synthesis of a functional membrane transporter into a tethered bilayer lipid membrane. Langmuir 32 (2016), 2445–2449.
-
(2016)
Langmuir
, vol.32
, pp. 2445-2449
-
-
Zieleniecki, J.L.1
Nagarajan, Y.2
Waters, S.3
Rongala, J.4
Thompson, V.5
Hrmova, M.6
Köper, I.7
-
24
-
-
85106461376
-
Cell-free synthesis meets antibody production: a review
-
24 Stech, M., Kubick, S., Cell-free synthesis meets antibody production: a review. Antibodies 4 (2015), 12–33.
-
(2015)
Antibodies
, vol.4
, pp. 12-33
-
-
Stech, M.1
Kubick, S.2
-
25
-
-
84959480044
-
Aptamer-based nanobiosensors
-
25 Kim, Y.S., Raston, N.H.A., Gu, M.B., Aptamer-based nanobiosensors. Biosens Bioelectron 76 (2016), 2–19.
-
(2016)
Biosens Bioelectron
, vol.76
, pp. 2-19
-
-
Kim, Y.S.1
Raston, N.H.A.2
Gu, M.B.3
-
26
-
-
84863610029
-
An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells
-
26 Shin, J., Noireaux, V., An E. coli cell-free expression toolbox: application to synthetic gene circuits and artificial cells. ACS Synth Biol 1 (2012), 29–41.
-
(2012)
ACS Synth Biol
, vol.1
, pp. 29-41
-
-
Shin, J.1
Noireaux, V.2
-
27
-
-
84966334405
-
The all E. coli TX-TL toolbox 2.0: a platform for cell-free synthetic biology
-
This study presents several components, concepts, and demonstrations important for developing applications of cell-free synthetic biology. The authors present different transcription factors, characterize components for tuning mRNA and protein dynamics, demonstrate a pulse generator, and assemble the violacene pathway. They also produce whole phages and ultimately show long duration expression in liposomes.
-
27•• Garamella, J., Marshall, R., Rustad, M., Noireaux, V., The all E. coli TX-TL toolbox 2.0: a platform for cell-free synthetic biology. ACS Synth Biol 5 (2016), 344–355 This study presents several components, concepts, and demonstrations important for developing applications of cell-free synthetic biology. The authors present different transcription factors, characterize components for tuning mRNA and protein dynamics, demonstrate a pulse generator, and assemble the violacene pathway. They also produce whole phages and ultimately show long duration expression in liposomes.
-
(2016)
ACS Synth Biol
, vol.5
, pp. 344-355
-
-
Garamella, J.1
Marshall, R.2
Rustad, M.3
Noireaux, V.4
-
28
-
-
18144363600
-
Engineering gene networks to emulate Drosophila embryonic pattern formation
-
28 Isalan, M., Lemerle, C., Serrano, L., Engineering gene networks to emulate Drosophila embryonic pattern formation. PLoS Biol, 3, 2005, e64.
-
(2005)
PLoS Biol
, vol.3
, pp. e64
-
-
Isalan, M.1
Lemerle, C.2
Serrano, L.3
-
29
-
-
84885968220
-
Multi-input regulation and logic with T7 promoters in cells and cell-free systems
-
29 Iyer, S., Karig, D.K., Norred, S.E., Simpson, M.L., Doktycz, M.J., Multi-input regulation and logic with T7 promoters in cells and cell-free systems. PLoS One, 8, 2013, e78442.
-
(2013)
PLoS One
, vol.8
, pp. e78442
-
-
Iyer, S.1
Karig, D.K.2
Norred, S.E.3
Simpson, M.L.4
Doktycz, M.J.5
-
30
-
-
0242268453
-
Principles of cell-free genetic circuit assembly
-
30 Noireaux, V., Bar-Ziv, R., Libchaber, A., Principles of cell-free genetic circuit assembly. Proc Natl Acad Sci 100 (2003), 12672–12677.
-
(2003)
Proc Natl Acad Sci
, vol.100
, pp. 12672-12677
-
-
Noireaux, V.1
Bar-Ziv, R.2
Libchaber, A.3
-
31
-
-
84949895685
-
Rapid cell-free forward engineering of novel genetic ring oscillators
-
31 Niederholtmeyer, H., Sun, Z.Z., Hori, Y., Yeung, E., Verpoorte, A., Murray, R.M., Maerkl, S.J., Rapid cell-free forward engineering of novel genetic ring oscillators. Elife, 4, 2015, e09771.
-
(2015)
Elife
, vol.4
, pp. e09771
-
-
Niederholtmeyer, H.1
Sun, Z.Z.2
Hori, Y.3
Yeung, E.4
Verpoorte, A.5
Murray, R.M.6
Maerkl, S.J.7
-
32
-
-
0037892386
-
Bioremediation: Nature's Way to a Cleaner Environment
-
US Geological Survey
-
32 Chapelle, F.H., Bioremediation: Nature's Way to a Cleaner Environment. 1995, US Geological Survey.
-
(1995)
-
-
Chapelle, F.H.1
-
33
-
-
78649900316
-
Genetically engineered oil-eating microbes for bioremediation: prospects and regulatory challenges
-
33 Ezezika, O.C., Singer, P.A., Genetically engineered oil-eating microbes for bioremediation: prospects and regulatory challenges. Technol Soc 32 (2010), 331–335.
-
(2010)
Technol Soc
, vol.32
, pp. 331-335
-
-
Ezezika, O.C.1
Singer, P.A.2
-
34
-
-
84993958585
-
ATLAS of biochemistry: a repository of all possible biochemical reactions for synthetic biology and metabolic engineering studies
-
34 Hadadi, N., Hafner, J., Shajkofci, A., Zisaki, A., Hatzimanikatis, V., ATLAS of biochemistry: a repository of all possible biochemical reactions for synthetic biology and metabolic engineering studies. ACS Synth Biol 5 (2016), 1155–1166.
-
(2016)
ACS Synth Biol
, vol.5
, pp. 1155-1166
-
-
Hadadi, N.1
Hafner, J.2
Shajkofci, A.3
Zisaki, A.4
Hatzimanikatis, V.5
-
35
-
-
84921496822
-
Cell-free metabolic engineering: biomanufacturing beyond the cell
-
35 Dudley, Q.M., Karim, A.S., Jewett, M.C., Cell-free metabolic engineering: biomanufacturing beyond the cell. Biotechnol J 10 (2015), 69–82.
-
(2015)
Biotechnol J
, vol.10
, pp. 69-82
-
-
Dudley, Q.M.1
Karim, A.S.2
Jewett, M.C.3
-
36
-
-
84957429636
-
Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase
-
36 Li, J., Lawton, T.J., Kostecki, J.S., Nisthal, A., Fang, J., Mayo, S.L., Rosenzweig, A.C., Jewett, M.C., Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase. Biotechnol J 11 (2016), 212–218.
-
(2016)
Biotechnol J
, vol.11
, pp. 212-218
-
-
Li, J.1
Lawton, T.J.2
Kostecki, J.S.3
Nisthal, A.4
Fang, J.5
Mayo, S.L.6
Rosenzweig, A.C.7
Jewett, M.C.8
-
37
-
-
85013198483
-
Design space exploration of the violacein pathway in Escherichia coli based transcription translation cell-free system (TX-TL)
-
37 Nguyen, P.H.B., Wu, Y., Guo, S., Murray, R.M., Design space exploration of the violacein pathway in Escherichia coli based transcription translation cell-free system (TX-TL). bioRxiv 027656 (2016), 1–10.
-
(2016)
bioRxiv
, vol.27656
, pp. 1-10
-
-
Nguyen, P.H.B.1
Wu, Y.2
Guo, S.3
Murray, R.M.4
-
38
-
-
84988579147
-
Portable, on-demand biomolecular manufacturing
-
e212
-
38 Pardee, K., Slomovic, S., Nguyen, P.Q., Lee, J.W., Donghia, N., Burrill, D., Ferrante, T., McSorley, F.R., Furuta, Y., Vernet, A., Portable, on-demand biomolecular manufacturing. Cell 167 (2016), 248–259 e212.
-
(2016)
Cell
, vol.167
, pp. 248-259
-
-
Pardee, K.1
Slomovic, S.2
Nguyen, P.Q.3
Lee, J.W.4
Donghia, N.5
Burrill, D.6
Ferrante, T.7
McSorley, F.R.8
Furuta, Y.9
Vernet, A.10
-
39
-
-
85006489426
-
Cell-free mixing of Escherichia coli crude extracts to prototype and rationally engineer high-titer mevalonate synthesis
-
Different cell-free extracts, each containing different pathway enzymes, were mixed to assemble and optimize a metabolic pathway. This study shows many benefits of the cell-free environment for facilitating pathway composition, pathway tuning (e.g., by using different plasmid rations), and enzyme screening. These techniques can ultimately be extended to implementing remediation pathways.
-
39•• Dudley, Q.M., Anderson, K.C., Jewett, M.C., Cell-free mixing of Escherichia coli crude extracts to prototype and rationally engineer high-titer mevalonate synthesis. ACS Synth Biol 5 (2016), 1578–1588 Different cell-free extracts, each containing different pathway enzymes, were mixed to assemble and optimize a metabolic pathway. This study shows many benefits of the cell-free environment for facilitating pathway composition, pathway tuning (e.g., by using different plasmid rations), and enzyme screening. These techniques can ultimately be extended to implementing remediation pathways.
-
(2016)
ACS Synth Biol
, vol.5
, pp. 1578-1588
-
-
Dudley, Q.M.1
Anderson, K.C.2
Jewett, M.C.3
-
40
-
-
84962739598
-
A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery
-
The authors combine different cell-free extracts to construct a metabolic pathway. They explore different co-factors as well as genetic modifications to the extract source strain. In addition, they test the effect of freeze drying and reconstituting the cell-free metabolic pathway.
-
40•• Karim, A.S., Jewett, M.C., A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery. Metab Eng 36 (2016), 116–126 The authors combine different cell-free extracts to construct a metabolic pathway. They explore different co-factors as well as genetic modifications to the extract source strain. In addition, they test the effect of freeze drying and reconstituting the cell-free metabolic pathway.
-
(2016)
Metab Eng
, vol.36
, pp. 116-126
-
-
Karim, A.S.1
Jewett, M.C.2
-
41
-
-
85013164425
-
-
Bioremediation of persistent organic pollutants using thermophilic bacteria. Google Patents; 2013.
-
41 O'Driscoll K, Sambrotto R, DiFilippo R, Piccillo P: Bioremediation of persistent organic pollutants using thermophilic bacteria. Google Patents; 2013.
-
-
-
O'Driscoll, K.1
Sambrotto, R.2
DiFilippo, R.3
Piccillo, P.4
-
42
-
-
84908703254
-
Purified cell-free systems as standard parts for synthetic biology
-
42 Matsubayashi, H., Ueda, T., Purified cell-free systems as standard parts for synthetic biology. Curr Opin Chem Biol 22 (2014), 158–162.
-
(2014)
Curr Opin Chem Biol
, vol.22
, pp. 158-162
-
-
Matsubayashi, H.1
Ueda, T.2
-
43
-
-
84965107861
-
Performance benchmarking of four cell-free protein expression systems
-
43 Gagoski, D., Polinkovsky, M.E., Mureev, S., Kunert, A., Johnston, W., Gambin, Y., Alexandrov, K., Performance benchmarking of four cell-free protein expression systems. Biotechnol Bioeng 113 (2016), 292–300.
-
(2016)
Biotechnol Bioeng
, vol.113
, pp. 292-300
-
-
Gagoski, D.1
Polinkovsky, M.E.2
Mureev, S.3
Kunert, A.4
Johnston, W.5
Gambin, Y.6
Alexandrov, K.7
-
44
-
-
84903211692
-
Gene circuit performance characterization and resource usage in a cell-free “breadboard”
-
44 Siegal-Gaskins, D., Tuza, Z.A., Kim, J., Noireaux, V., Murray, R.M., Gene circuit performance characterization and resource usage in a cell-free “breadboard”. ACS Synth Biol 3 (2014), 416–425.
-
(2014)
ACS Synth Biol
, vol.3
, pp. 416-425
-
-
Siegal-Gaskins, D.1
Tuza, Z.A.2
Kim, J.3
Noireaux, V.4
Murray, R.M.5
-
45
-
-
84941731930
-
Characterizing and prototyping genetic networks with cell-free transcription–translation reactions
-
The authors discuss approaches for using cell-free systems to prototype genetic networks and present an RNA transcriptional cascade and a feed-forward loop in a cell-free protein expression system. They compare in vitro and in vivo performance.
-
45• Takahashi, M.K., Hayes, C.A., Chappell, J., Sun, Z.Z., Murray, R.M., Noireaux, V., Lucks, J.B., Characterizing and prototyping genetic networks with cell-free transcription–translation reactions. Methods 86 (2015), 60–72 The authors discuss approaches for using cell-free systems to prototype genetic networks and present an RNA transcriptional cascade and a feed-forward loop in a cell-free protein expression system. They compare in vitro and in vivo performance.
-
(2015)
Methods
, vol.86
, pp. 60-72
-
-
Takahashi, M.K.1
Hayes, C.A.2
Chappell, J.3
Sun, Z.Z.4
Murray, R.M.5
Noireaux, V.6
Lucks, J.B.7
-
46
-
-
84909607936
-
A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system
-
46 Caschera, F., Noireaux, V., A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system. Metab Eng 27 (2015), 29–37.
-
(2015)
Metab Eng
, vol.27
, pp. 29-37
-
-
Caschera, F.1
Noireaux, V.2
-
47
-
-
79251480753
-
Coarse-grained dynamics of protein synthesis in a cell-free system
-
47 Karzbrun, E., Shin, J., Bar-Ziv, R.H., Noireaux, V., Coarse-grained dynamics of protein synthesis in a cell-free system. Phys Rev Lett, 106, 2011, 048104.
-
(2011)
Phys Rev Lett
, vol.106
, pp. 048104
-
-
Karzbrun, E.1
Shin, J.2
Bar-Ziv, R.H.3
Noireaux, V.4
-
48
-
-
78649788244
-
Efficient cell-free expression with the endogenous E. coli RNA polymerase and sigma factor 70
-
48 Shin, J., Noireaux, V., Efficient cell-free expression with the endogenous E. coli RNA polymerase and sigma factor 70. J Biol Eng, 4, 2010, 1.
-
(2010)
J Biol Eng
, vol.4
, pp. 1
-
-
Shin, J.1
Noireaux, V.2
-
49
-
-
84912105277
-
Biodegradation of dimethyl phthalate ester using free cells, entrapped cells of Variovorax sp. BS1 and cell free enzyme extracts: a comparative study
-
49 Prasad, B., Suresh, S., Biodegradation of dimethyl phthalate ester using free cells, entrapped cells of Variovorax sp. BS1 and cell free enzyme extracts: a comparative study. Int Biodeterior Biodegrad 97 (2015), 179–187.
-
(2015)
Int Biodeterior Biodegrad
, vol.97
, pp. 179-187
-
-
Prasad, B.1
Suresh, S.2
-
51
-
-
85013143466
-
-
Method for producing complex multienzymatical, storage resistant reaction mixtures and use thereof. Google Patents; 2002.
-
51 Peters LE, Bendzko P: Method for producing complex multienzymatical, storage resistant reaction mixtures and use thereof. Google Patents; 2002.
-
-
-
Peters, L.E.1
Bendzko, P.2
-
52
-
-
84898432799
-
Lyophilized Escherichia coli-based cell-free systems for robust, high-density, long-term storage
-
52 Smith, M.T., Berkheimer, S.D., Werner, C.J., Bundy, B.C., Lyophilized Escherichia coli-based cell-free systems for robust, high-density, long-term storage. Biotechniques 56 (2014), 186–193.
-
(2014)
Biotechniques
, vol.56
, pp. 186-193
-
-
Smith, M.T.1
Berkheimer, S.D.2
Werner, C.J.3
Bundy, B.C.4
-
53
-
-
84957434998
-
Cell-free protein synthesis of a cytotoxic cancer therapeutic: onconase production and a just-add-water cell-free system
-
53 Salehi, A.S., Smith, M.T., Bennett, A.M., Williams, J.B., Pitt, W.G., Bundy, B.C., Cell-free protein synthesis of a cytotoxic cancer therapeutic: onconase production and a just-add-water cell-free system. Biotechnol J 11 (2016), 274–281.
-
(2016)
Biotechnol J
, vol.11
, pp. 274-281
-
-
Salehi, A.S.1
Smith, M.T.2
Bennett, A.M.3
Williams, J.B.4
Pitt, W.G.5
Bundy, B.C.6
-
54
-
-
85013164421
-
Heat-stable preservation of protein expression systems for portable therapeutics production
-
54 Karig, D.K., Bessling, S., Thielen, P., Zhang, S., Wolfe, J., Heat-stable preservation of protein expression systems for portable therapeutics production. bioRxiv 089664 (2016), 1–27.
-
(2016)
bioRxiv
, vol.89664
, pp. 1-27
-
-
Karig, D.K.1
Bessling, S.2
Thielen, P.3
Zhang, S.4
Wolfe, J.5
-
55
-
-
84957428307
-
A cell-free expression and purification process for rapid production of protein biologics
-
55 Sullivan, C.J., Pendleton, E.D., Sasmor, H.H., Hicks, W.L., Farnum, J.B., Muto, M., Amendt, E.M., Schoborg, J.A., Martin, R.W., Clark, L.G., A cell-free expression and purification process for rapid production of protein biologics. Biotechnol J 11 (2016), 238–248.
-
(2016)
Biotechnol J
, vol.11
, pp. 238-248
-
-
Sullivan, C.J.1
Pendleton, E.D.2
Sasmor, H.H.3
Hicks, W.L.4
Farnum, J.B.5
Muto, M.6
Amendt, E.M.7
Schoborg, J.A.8
Martin, R.W.9
Clark, L.G.10
-
56
-
-
84954341987
-
Toward microfluidic reactors for cell-free protein synthesis at the point-of-care
-
56 Timm, A.C., Shankles, P.G., Foster, C.M., Doktycz, M.J., Retterer, S.T., Toward microfluidic reactors for cell-free protein synthesis at the point-of-care. Small 12 (2016), 810–817.
-
(2016)
Small
, vol.12
, pp. 810-817
-
-
Timm, A.C.1
Shankles, P.G.2
Foster, C.M.3
Doktycz, M.J.4
Retterer, S.T.5
-
57
-
-
84940489518
-
The PURE system for the cell-free synthesis of membrane proteins
-
57 Kuruma, Y., Ueda, T., The PURE system for the cell-free synthesis of membrane proteins. Nat Protoc 10 (2015), 1328–1344.
-
(2015)
Nat Protoc
, vol.10
, pp. 1328-1344
-
-
Kuruma, Y.1
Ueda, T.2
-
58
-
-
69049107340
-
Silica-coated alginate beads for in vitro protein synthesis via transcription/translation machinery encapsulation
-
58 Lim, S.Y., Kim, K.-O., Kim, D.-M., Park, C.B., Silica-coated alginate beads for in vitro protein synthesis via transcription/translation machinery encapsulation. J Biotechnol 143 (2009), 183–189.
-
(2009)
J Biotechnol
, vol.143
, pp. 183-189
-
-
Lim, S.Y.1
Kim, K.-O.2
Kim, D.-M.3
Park, C.B.4
-
59
-
-
84974831419
-
DNA microgels as a platform for cell-free protein expression and display
-
59 Kahn, J.S., Ruiz, R.C., Sureka, S., Peng, S., Derrien, T.L., An, D., Luo, D., DNA microgels as a platform for cell-free protein expression and display. Biomacromolecules 17 (2016), 2019–2026.
-
(2016)
Biomacromolecules
, vol.17
, pp. 2019-2026
-
-
Kahn, J.S.1
Ruiz, R.C.2
Sureka, S.3
Peng, S.4
Derrien, T.L.5
An, D.6
Luo, D.7
-
60
-
-
85027933472
-
Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements
-
60 Kelwick, R., Webb, A.J., MacDonald, J.T., Freemont, P.S., Development of a Bacillus subtilis cell-free transcription-translation system for prototyping regulatory elements. Metab Eng 38 (2016), 370–381.
-
(2016)
Metab Eng
, vol.38
, pp. 370-381
-
-
Kelwick, R.1
Webb, A.J.2
MacDonald, J.T.3
Freemont, P.S.4
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