Rapid screening of quorum-sensing signal N-acyl homoserine lactones by an in vitro cell-free assay

Applied and Environmental Microbiology

Volumn 74, Issue 12, 2008, Pages 3667-3671

  Kawaguchi, Tomohiro ^a   Yung, Pin Chen ^a   Norman, R Sean ^a   Decho, Alan W ^a  

^a UNIVERSITY OF SOUTH CAROLINA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIOLOGY; BIOSENSORS; CHEMICALS; COMPUTER NETWORKS; DETECTORS; NETWORK PROTOCOLS; SIGNAL TRANSDUCTION;

ACYL-HOMOSERINE LACTONE (ACYL-HSL); AGROBACTERIUM TUMEFACIENS (PROPAT); AMERICAN SOCIETY; ASSAY DETECTION; AUTOINDUCERS; BIO SENSOR; CELL-FREE ASSAYS; CHROMOGENIC SUBSTRATES; GALACTOPYRANOSIDE; GALACTOSIDASE; HIGH SENSITIVITY (HS); HIGH-THROUGHPUT (HT); IN-CELL; IN-VITRO; METAGENOMIC LIBRARIES; N ACYL HOMOSERINE LACTONES; QUORUM SENSING (QS); RAPID SCREENING; WHOLE CELL BIOSENSOR;

CELL CULTURE;

5 BROMO 4 CHLORO 3 INDOLYL BETA DEXTRO GALACTOPYRANOSIDE; BETA GALACTOSIDASE; N ACYLHOMOSERINE LACTONE;

BACTERIUM; BIOASSAY; DETECTION METHOD; SIGNALING;

ANALYTIC METHOD; ARTICLE; ASSAY; BIOSENSOR; CELL FREE ASSAY; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; QUORUM SENSING; RHIZOBIUM RADIOBACTER;

ACYL-BUTYROLACTONES; BETA-GALACTOSIDASE; BIOSENSING TECHNIQUES; CELL-FREE SYSTEM; DENSITOMETRY; GALACTOSIDES; INDOLES; LUMINESCENT AGENTS; LUMINESCENT MEASUREMENTS; QUORUM SENSING; RHIZOBIUM RADIOBACTER; SENSITIVITY AND SPECIFICITY; TIME FACTORS;

AGROBACTERIUM TUMEFACIENS; BACTERIA (MICROORGANISMS);

EID: 45749152670     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.02869-07     Document Type: Article

References (43)

1. Andersen, J. B., A. Heydorn, M. Hentzer, L. Eberl, O. Geinsenberger, B. B. Christensen, S. Molin, and M. Givskov. 2001. gfp-based N-acyl homoserine lactone sensor system for detection of bacterial communication. Appl. Environ. Microbiol. 67:575-585.
2. Bassler, B. L. 2002. Small talk: cell-to-cell communication in bacteria. Cell 109:421-424.
3. Bradford, M. M. 1976. A rapid and sensitive method for the quantification of microgram quantity of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
4. Brock, T. D. 1964. Action of streptomycin and related antibiotics. Fed. Proc. 23:965-975.
5. Camara, M., P. Williams, and A. Hardman. 2002. Controlling infection by tuning in and tuning down the volume of bacterial small-talk. Lancet Infect. Dis. 2:667-676.
6. Cataldi, T. R. I., G. Bianco, L. Palazzo, and V. Quaranta. 2007. Occurrence of N-acyl-L-homoserine lactones in extracts of some gram-negative bacteria evaluated by gas chromatography-mass spectrometry. Anal. Biochem. 361:226-235.
7. Chen, H. Z., and G. Zubay. 1983. Prokaryotic coupled transcription-translation. Methods Enzymol. 101:674-690.
8. Ezure, T., T. Suzuki, S. Higashide, E. Shintami, K. Endo, S. Kobayashi, M. Shikata, M. Ito, K. Tanimizu, and O. Nishimura. 2006. Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol. Prog. 22:1570-1577.
9. Fuqua, C., and E. P. Greenberg. 2002. Listening in on bacteria: acyl-homoserine lactone signalling. Nat. Rev. Mol. Cell Biol. 3:685-695.
10. Fuqua, C., and S. C. Winans. 1996. Conserved cis-acting promoter elements are required for density-dependent transcription of Agrobacterium tumefaciens conjugal transfer genes. J. Bacteriol. 178:435-440.
11. Fuqua, W. C., and S. C. Winans. 1994. A LuxR-LuxI type regulatory system activates Agrobacterium Ti plasmid conjugal transfer in the presence of a plant tumor metabolite. J. Bacteriol. 176:2796-2806.
12. González, J. E., and N. D. Keshavan. 2006. Messing with bacterial quorum sensing. Microbiol. Mol. Biol. Rev. 70:859-875.
13. Jackson, R. J., and T. Hunt. 1983. Preparation and use of nuclease-treated rabbit reticulocyte lysates for translation of eukaryotic messenger RNA. Methods Enzymol. 96:50-74.
14. Jafra, S., and J. M. van der Wolf. 2004. Fast screening method for detection of acyl-HSL-degrading soil isolates. J. Microbiol. Methods 57:415-420.
15. Kim, D. M., T. Kigawa, C. Y. Choi, and S. Yokaoyama. 1996. A highly efficient cell-free protein synthesis system from Escherichia coli. Eur. J. Biochem. 239:881-886.
16. Luo, Z. Q., Y. Quin, and S. K. Farrand. 2000. The antiactivator traM interferes with the autoinducer-dependent binding of traR to DNA by interacting with the C-terminal region of the quorum sensing activator. J. Biol. Chem. 275:7713-7722.
17. Luo, Z. Q., T. E. Clemente, and S. K. Farrand. 2001. Construction of a derivative of Agrobacterium tumefaciens C58 that does not mutate to tetracycline resistance. Mol. Plant-Microbe Interact. 14:98-103.
18. Luo, Z. Q., S. Su, and S. K. Farrand. 2003. In situ activation of the quorumsensing transcription factor TraR by cognate and noncognate acyl-homoserine lactone ligands: kinetics and consequences. J. Bacteriol. 185:5665-5672.
19. McClean, K. H., M. K. Winson, L. Fish, A. Taylor, S. R. Chabra, M. Camara, M. Daykin, J. H. Lamb, S. Swift, B. W. Bycroft, G. S. A. B. Stewart, and P. Williams. 1997. Quorum sensing and Chromobacterium violaceum: exploitation of violacin production and inhibition for the detection of N-acylhomoserine lactones. Microbiology 143:3703-3711.
20. Miller, M. B., and B. L. Bassler. 2001. Quorum sensing in bacteria. Annu. Rev. Microbiol. 55:165-199.
21. Morin, D., B. Grasland, K. Vallée-Réhel, C. Dufau, and D. Haras. 2003. On-line high-performance liquid chromatography-mass spectrometric detection and quantification of N-acylhomoserine lactones, quorum sensing signal molecules in the presence of biological matrices. J. Chromatogr. A 1002:79-92.
22. Moskowitz, M. 1963. Differences in the precipitability of nucleic acids with streptomycin and dihydrostreptomycin. Nature 200:335-337.
23. Olliver, L., and C. D. Boyd. 1998. In vitro translation of messenger RNA in a rabbit reticulocyte lysate cell-free system. Methods Mol. Biol. 86:221-227.
24. Piper, K. R., and S. K. Farrand. 2000. Quorum sensing but not autoinduction of Ti plasmid conjugal transfer requires control by opine regulon and the antiactivator TraM. J. Bacteriol. 182:1080-1088.
25. Qin, Y., S. Su, and S. K. Farrand. 2007. Molecular basis of transcriptional antiactivation. J. Biol. Chem. 282:19979-19991.
26. Shaw, P. D., G. Ping, S. L. Daly, C. Cha, J. E. Cronan, Jr., K. L. Rinehart, and S. K. Farrand. 1997. Detecting and characterizing N-acyl-homoserine lactone signal molecules by thin-layer chromatography. Proc. Natl. Acad. Sci. USA 94:6036-6041.
27. Singh, M. P., and M. Greenstein. 2006. A simple, rapid, sensitive method detecting homoserine lactone (HSL)-related compounds in microbial extracts. J. Microbiol. Methods 65:32-37.
28. Snyder, L., and W. Champness. 2002. Molecular genetics of bacteria, 2nd ed. ASM Press, Washington, DC.
29. Spelsberg, T. C. 1983. A rapid method for analysis of ligand binding to deoxyribonucleic acid and soluble nucleoproteins using streptomycin: application to steroid receptor ligands. Biochemistry 22:12-21.
30. Spirin, A. S., V. I. Baranov, L. A. Ryabova, S. Y. Ovodov, and Y. B. Alakhov. 1988. A continuous cell-free translation system capable of producing polypeptides in high yield. Science 242:1162-1164.
31. Steindler, L., and V. Venturi. 2007. Detection of quorum-sensing N-acyl homoserine lactone signal molecules by bacterial biosensors. FEMS Microbiol. Lett. 266:1-9.
32. Swerdel, M. R., and A. M. Fallon. 1989. Cell-free translation in lysates from Spodoplera frugiperda (Lepidoptera: Noctuidae) cells. Comp. Biochem. Physiol. 93B:803-806.
33. Swiderska, A., A. K. Berndtson, M. R. Cha, L. Li, G. M. Beaudoin, J. Zhu, and C. Fuqua. 2001. Inhibition of the Agrobacterium tumefaciencs traR quorum sensing regulator - interactions with the traM anti-activator. J. Biol. Chem. 276:49449-49458.
34. Tait, K., I. Joint, M. Daykin, D. L. Milton, P. Williams, and M. Cámara. 2005. Disruption of quorum sensing in seawater abolishes attraction of zoospores of the green alga Ulva to bacterial biofilms. Environ. Microbiol. 7:229-240.
35. Tempe, J., A. Petit, M. Holsters, M. Montagu, and J. Schell. 1977. Thermosensitive step associated with transfer of the Ti plasmid during conjugation: possible relation to transformation in crown gall. Proc. Natl. Acad. Sci. USA 74:2848-2849.
36. Wang, Y. J., and J. R. Leadbetter. 2005. Rapid acyl-homoserine lactone quorum signal decomposition by diverse soils. Appl. Environ. Microbiol. 71:1291-1299.
37. Waters, C. M., and B. L. Bassler. 2005. Quorum sensing: cell-to-cell communication in bacteria. Annu. Rev. Cell Dev. Biol. 21:319-346.
38. Whitehead, N. A., A. M. L. Barnard, H. Slater, N. J. L. Simpson, and G. P. C. Salmond. 2001. Quorum sensing in Gram-negative bacteria. FEMS Microbiol. Rev. 25:365-404.
39. Winson, M. K., S. Swift, L. Fish, J. P. Throup, F. Jørgensen, S. R. Chhabra, B. W. Bycroft, P. Williams, and G. S. A. B. Stewart. 1998. Construction of luxCABE-based plasmid sensors for investigating N-acyl homoserine lactone mediated quorum sensing. FEMS Microbiol. Lett. 163:185-192.
40. Wu, H., Z. Song, N. Hoiby, and M. Givskov. 2004. Quorum sensing in Gram-negative bacteria. Prog. Nat. Sci. 14:377-387.
41. Yan, L., M. S. Allen, M. L. Simpson, G. S. Sayler, and C. D. Cox. 2007. Direct quantification of N-(3-oxo-hexanoyl)-L- homoserine lactone in culture supernatant using a whole-cell bioreporter. J. Microbiol. Methods 68:40-45.
42. Yang, Y.-H., T. H. Lee, J. H. Kim, E. J. Kim, H. S. Joo, C. S. Lee, and B. G. Kim. 2006. High-throughput detection method of quorum-sensing molecules by colorimetry and its applications. Anal. Biochem. 356:297-299.
43. Zhu, J., Y. Chai, Z. Zhong, S. Li, and S. C. Winans. 2003. Agrobacterium bioassay strain for ultrasensitive detection of N-acylhomoserine lactone-type quorum-sensing molecules: detection of autoinducers in Mesorhizobium huakuii. Appl. Environ. Microbiol. 69:6949-6953.