Engineering an allosteric transcription factor to respond to new ligands

Nature Methods

Volumn 13, Issue 2, 2016, Pages 177-183

  Taylor, Noah D ^a,b   Garruss, Alexander S ^a,b   Moretti, Rocco ^c,d,g   Chan, Sum ^e   Arbing, Mark A ^e   Cascio, Duilio ^e   Rogers, Jameson K ^a,b   Isaacs, Farren J ^f   Kosuri, Sriram ^e   Baker, David ^c,d   Fields, Stanley ^d   Church, George M ^a,b   Raman, Srivatsan ^a,b,h  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF WASHINGTON   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f YALE UNIVERSITY   (United States)

^g VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

^h UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; COLICIN E1; CYSTEINE; DEOXYRIBONUCLEASE I; DNA; FUCOSE; GENTIOBIOSE; GLUTAMINE; GLYCINE; ISOLEUCINE; ISOPROPYL THIOGALACTOSIDE; LACTITOL; LEUCINE; METHIONINE; OLIGONUCLEOTIDE; PHENYLALANINE DERIVATIVE; PORIN; SUCRALOSE; THREONINE; TISSUE FACTOR PATHWAY INHIBITOR; TRYPTOPHAN DERIVATIVE; TYROSINE; BACTERIAL DNA; DISACCHARIDE; ESCHERICHIA COLI PROTEIN; LAC REPRESSOR; LACI PROTEIN, E COLI; PROTEIN BINDING; SUCROSE; SUGAR ALCOHOL; TRICHLOROSUCROSE;

ALLOSTERISM; AMINO ACID SUBSTITUTION; ANALYTICAL ERROR; ARTICLE; BACTERIAL GENOME; CELL METABOLISM; CODON; CONFORMATION; CONTROLLED STUDY; CRYSTAL STRUCTURE; CYTOLOGY; DIMERIZATION; DNA BINDING; DNA SYNTHESIS; ESCHERICHIA COLI; FRAMESHIFT MUTATION; GENETIC CODE; GENETIC TRANSCRIPTION; GENETIC VARIABILITY; HIGH THROUGHPUT SEQUENCING; HYDROGEN BOND; LIGAND BINDING; METABOLIC ENGINEERING; MOLECULAR INTERACTION; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INDUCTION; ANALOGS AND DERIVATIVES; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; METABOLISM; MOLECULAR MODEL; MUTATION; PROTEIN CONFORMATION;

ALLOSTERIC REGULATION; DISACCHARIDES; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; FUCOSE; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; LAC REPRESSORS; MODELS, MOLECULAR; MUTATION; PROTEIN BINDING; PROTEIN CONFORMATION; SUCROSE; SUGAR ALCOHOLS;

EID: 84956609810     PISSN: 15487091     EISSN: 15487105     Source Type: Journal    
DOI: 10.1038/nmeth.3696     Document Type: Article

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