A cost-effective polyphosphate-based metabolism fuels an all E. coli cell-free expression system

Metabolic Engineering

Volumn 27, Issue , 2015, Pages 29-37

  Caschera, Filippo ^a   Noireaux, Vincent ^a  

^a University of Minnesota   (United States)

Author keywords

Cell free transcription translation; E. coli; Hexametaphosphate; Maltodextrin; Metabolism; Phosphorylation

Indexed keywords

BIOSYNTHESIS; CELLS; COST EFFECTIVENESS; COSTS; CYTOLOGY; ESCHERICHIA COLI; MACHINERY; METABOLIC ENGINEERING; METABOLISM; PHOSPHORYLATION; PHYSIOLOGY; POLYSACCHARIDES; PROTEINS; SYNTHESIS (CHEMICAL); TRANSCRIPTION;

CELL FREE PROTEIN SYNTHESIS; CELL-FREE; CELL-FREE EXPRESSION; E. COLI; HEXAMETAPHOSPHATE; INORGANIC PHOSPHATES; MALTODEXTRINS; PHOSPHOENOLPYRUVATES;

CELL ENGINEERING;

CREATINE PHOSPHATE; FUEL; PHOSPHOENOLPYRUVATE; POLYPHOSPHATE; ADENOSINE TRIPHOSPHATE; COMPLEX MIXTURES; POLYPHOSPHATES; RECOMBINANT PROTEINS;

ARTICLE; BACTERIOPHAGE; CELL FREE EXPRESSION SYSTEM; CELL FREE SYSTEM; COST EFFECTIVENESS ANALYSIS; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; IN VITRO STUDY; METABOLIC ENGINEERING; MICROPLATE READER; MOLECULAR WEIGHT; NONHUMAN; PROTEIN PHOSPHORYLATION; PROTEIN SYNTHESIS; BACTERIOPHAGES; BIOSYNTHESIS; CHEMISTRY; COMPLEX MIXTURES; GENETICS; METABOLISM; PROTEIN BIOSYNTHESIS; TRANSCRIPTION, GENETIC;

ADENOSINE TRIPHOSPHATE; BACTERIOPHAGES; COMPLEX MIXTURES; ESCHERICHIA COLI; POLYPHOSPHATES; PROTEIN BIOSYNTHESIS; RECOMBINANT PROTEINS; TRANSCRIPTION, GENETIC;

EID: 84909607936     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2014.10.007     Document Type: Article

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