Cell-Free Synthesis of a Functional Membrane Transporter into a Tethered Bilayer Lipid Membrane

Langmuir

Volumn 32, Issue 10, 2016, Pages 2445-2449

  Zieleniecki, Julius L ^a   Nagarajan, Yagnesh ^b   Waters, Shane ^b   Rongala, Jay ^b   Thompson, Vanessa ^a   Hrmova, Maria ^b   Köper, Ingo ^a  

^a Flinders University ^*  (Australia)

^b UNIVERSITY OF ADELAIDE   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CELL MEMBRANES; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; LIPIDS; PROTEINS;

BILAYER LIPID MEMBRANE; CELL FREE SYNTHESIS; EUKARYOTIC CELLS; FUNCTIONAL FORMS; FUNCTIONAL MEMBRANES; FUNCTIONALIZED; IMMUNOBLOT ANALYSIS; PROTEIN SYNTHESIS;

BIOSYNTHESIS;

1,2-DIPHYTANOYLPHOSPHATIDYLCHOLINE; BORIC ACID; CARRIER PROTEIN; ESTER; LIPID BILAYER; MESSENGER RNA; PHOSPHATIDYLCHOLINE; PLANT PROTEIN; PLANT RNA; THIOCTIC ACID;

BIOSYNTHESIS; CELL FREE SYSTEM; CHEMISTRY; ELECTROCHEMICAL IMPEDANCE SPECTROSCOPY; GENETICS; HORDEUM; IMMUNOBLOTTING; LIPID BILAYER; METABOLISM; PH;

BORIC ACIDS; CELL-FREE SYSTEM; DIELECTRIC SPECTROSCOPY; ESTERS; HORDEUM; HYDROGEN-ION CONCENTRATION; IMMUNOBLOTTING; LIPID BILAYERS; MEMBRANE TRANSPORT PROTEINS; PHOSPHATIDYLCHOLINES; PLANT PROTEINS; RNA, MESSENGER; RNA, PLANT; THIOCTIC ACID;

EID: 84961223490     PISSN: 07437463     EISSN: 15205827     Source Type: Journal    
DOI: 10.1021/acs.langmuir.5b04059     Document Type: Article

References (33)

1. Sackmann, E. Supported membranes: Scientific and practical applications Science 1996, 271, 43-48 10.1126/science.271.5245.43
2. Chan, Y. H. M.; Boxer, S. G. Model membrane systems and their applications Curr. Opin. Chem. Biol. 2007, 11 (6) 581-587 10.1016/j.cbpa.2007.09.020
3. Castellana, E. T.; Cremer, P. S. Solid supported lipid bilayers: From biophysical studies to sensor design Surf. Sci. Rep. 2006, 61 (10) 429-444 10.1016/j.surfrep.2006.06.001
4. Köper, I. Insulating tethered bilayer lipid membranes to study membrane proteins Mol. BioSyst. 2007, 3, 651-657 10.1039/b707168j
5. Winterhalter, M. Black lipid membranes Curr. Opin. Colloid Interface Sci. 2000, 5, 250-255 10.1016/S1359-0294(00)00063-7
6. Holt, S. A.; Le Brun, A. P.; Majkrzak, C. F.; McGillivray, D. J.; Heinrich, F.; Losche, M.; Lakey, J. H. An ion-channel-containing model membrane: structural determination by magnetic contrast neutron reflectometry Soft Matter 2009, 5 (13) 2576-2586 10.1039/b822411k
7. Vockenroth, I. K.; Rossi, C.; Shah, M. R.; Koper, I. Formation of tethered bilayer lipid membranes probed by various surface sensitive techniques Biointerphases 2009, 4 (2) 19-26 10.1116/1.3122019
8. Cornell, B. A.; Braach-Maksvytis, V. L. B.; King, L. G.; Osman, P. D. J.; Raguse, B.; Wieczorek, L.; Pace, R. J. A biosensor that uses ion-channel switches Nature 1997, 387 (6633) 580-583 10.1038/42432
9. Raguse, B.; Braach-Maksvytis, V.; Cornell, B. A.; King, L. G.; Osman, P. D. J.; Pace, R. J.; Wieczorek, L. Tethered lipid bilayer membranes: fomation and ionic reservoir characterization Langmuir 1998, 14, 648-659 10.1021/la9711239
10. Terrettaz, S.; Mayer, M.; Vogel, H. Highly electrically insulating tethered lipid bilayers for probing the function of ion channel proteins Langmuir 2003, 19, 5567-5569 10.1021/la034197v
11. Krishna, G.; Schulte, J.; Cornell, B. A.; Pace, R. J.; Osman, P. D. Tethered Bilayer Membranes Containing Ionic Reservoirs: Selectivity and Conductance Langmuir 2003, 19, 2294-2305 10.1021/la026238d
12. Kwak, K. J.; Valincius, G.; Liao, W. C.; Hu, X.; Wen, X. J.; Lee, A.; Yu, B.; Vanderah, D. J.; Lu, W.; Lee, L. J. Formation and Finite Element Analysis of Tethered Bilayer Lipid Structures Langmuir 2010, 26 (23) 18199-18208 10.1021/la1021802
13. Tanaka, M.; Sackmann, E. Polymer-supported membranes as models of the cell surface Nature 2005, 437 (7059) 656-663 10.1038/nature04164
14. McGillivray, D. J.; Valincius, G.; Vanderah, D. J.; Febo-Ayala, W.; Woodward, J. T.; Heinrich, F.; Kasianowicz, J. J.; Losche, M. Molecular-scale structural and functional characterization of sparsely tethered bilayer lipid membranes Biointerphases 2007, 2 (1) 21-33 10.1116/1.2709308
15. Jeuken, L. J. C. Electrodes for integral membrane enzymes Nat. Prod. Rep. 2009, 26 (10) 1234-1240 10.1039/b903252e
16. Kohli, N.; Hassler, B. L.; Parthasarathy, L.; Richardson, R. J.; Ofoli, R. Y.; Worden, R. M.; Lee, I. Tethered lipid bilayers on electrolessly deposited gold for bioelectronic applications Biomacromolecules 2006, 7 (12) 3327-3335 10.1021/bm0603995
17. He, M. Cell-free protein synthesis: applications in proteomics and biotechnology New Biotechnol. 2008, 25 (2-3) 126-132 10.1016/j.nbt.2008.08.004
18. Periasamy, A.; Shadiac, N.; Amalraj, A.; Garajova, S.; Nagarajan, Y.; Waters, S.; Mertens, H. D.; Hrmova, M. Cell-free protein synthesis of membrane (1,3)-beta-d-glucan (Curdlan) synthase: co-translational insertion in liposomes and reconstitution in nanodiscs Biochim. Biophys. Acta, Biomembr. 2013, 1828 (2) 743-57 10.1016/j.bbamem.2012.10.003
19. Shadiac, N.; Nagarajan, Y.; Waters, S.; Hrmova, M. Close allies in membrane protein research: cell-free synthesis and nanotechnology Mol. Membr. Biol. 2013, 30 (3) 229-45 10.3109/09687688.2012.762125
20. Sutton, T.; Baumann, U.; Hayes, J.; Collins, N. C.; Shi, B. J.; Schnurbusch, T.; Hay, A.; Mayo, G.; Pallotta, M.; Tester, M.; Langridge, P. Boron-toxicity tolerance in barley arising from efflux transporter amplification Science 2007, 318 (5855) 1446-9 10.1126/science.1146853
21. Chalmeau, J.; Monina, N.; Shin, J.; Vieu, C.; Noireaux, V. alpha-Hemolysin pore formation into a supported phospholipid bilayer using cell-free expression Biochim. Biophys. Acta, Biomembr. 2011, 1808 (1) 271-8 10.1016/j.bbamem.2010.07.027
22. Ritz, S.; Hulko, M.; Zerfass, C.; May, S.; Hospach, I.; Krasteva, N.; Nelles, G.; Sinner, E. K. Cell-free expression of a mammalian olfactory receptor and unidirectional insertion into small unilamellar vesicles (SUVs) Biochimie 2013, 95 (10) 1909-16 10.1016/j.biochi.2013.06.021
23. Robelek, R.; Lemker, E. S.; Wiltschi, B.; Kirste, V.; Naumann, R.; Oesterhelt, D.; Sinner, E. K. Incorporation of in vitro synthesized GPCR into a tethered artificial lipid membrane system Angew. Chem., Int. Ed. 2007, 46 (4) 605-608 10.1002/anie.200602231
24. Coutable, A.; Thibault, C.; Chalmeau, J.; François, J. M.; Vieu, C.; Noireaux, V.; Trévisiol, E. Preparation of Tethered-Lipid Bilayers on Gold Surfaces for the Incorporation of Integral Membrane Proteins Synthesized by Cell-Free Expression Langmuir 2014, 30 (11) 3132-3141 10.1021/la5004758
25. Yildiz, A. A.; Knoll, W.; Gennis, R. B.; Sinner, E. K. Cell-free synthesis of cytochrome bo(3) ubiquinol oxidase in artificial membranes Anal. Biochem. 2012, 423 (1) 39-45 10.1016/j.ab.2012.01.007
26. Sutton, T.; Baumann, U.; Hayes, J.; Collins, N. C.; Shi, B. J.; Schnurbusch, T.; Hay, A.; Mayo, G.; Pallotta, M.; Tester, M.; Langridge, P. Boron-toxicity tolerance in barley arising from efflux transporter amplification Science 2007, 318, 1446-1449 10.1126/science.1146853
27. Nagarajan, Y.; Rongala, J.; Luang, S.; Singh, A.; Shadiac, N.; Hayes, J.; Sutton, T.; Gilliham, M.; Tyerman, S.; McPhee, G.; Voelcker, N. H.; Mertens, H. D. T.; Kirby, N.; Lee, J.-G.; Yingling, Y. G.; Hrmova, M. Na+-Dependent Anion Transport by a Barley Efflux Protein Revealed through an Integrative Platform Plant Cell 2015, 28 (1) 202-218 10.1105/tpc.15.00625
28. Atanasov, V.; Atanasova, P. P.; Vockenroth, I. K.; Knorr, N.; Koper, I. A molecular toolkit for highly insulating tethered bilayer lipid membranes on various substrates Bioconjugate Chem. 2006, 17 (3) 631-637 10.1021/bc050328n
29. Schnurbusch, T.; Hayes, J.; Hrmova, M.; Baumann, U.; Ramesh, S. A.; Tyerman, S. D.; Langridge, P.; Sutton, T. Boron Toxicity Tolerance in Barley through Reduced Expression of the Multifunctional Aquaporin HvNIP2;1 Plant Physiol. 2010, 153 (4) 1706-1715 10.1104/pp.110.158832
30. Saier, M. H.; Reddy, V. S.; Tamang, D. G.; Västermark, Å. The Transporter Classification Database Nucleic Acids Res. 2014, 42 (D1) D251-D258 10.1093/nar/gkt1097
31. Steinem, C.; Janshoff, A.; Ulrich, W.-P.; Sieber, M.; Galla, H.-J. Impedance analysis of supported lipid bilayer membranes: a scutiny of different preparation techniques Biochim. Biophys. Acta, Biomembr. 1996, 1279, 169-180 10.1016/0005-2736(95)00274-X
32. Hayes, J. E.; Reid, R. J. Boron Tolerance in Barley Is Mediated by Efflux of Boron from the Roots Plant Physiol. 2004, 136 (2) 3376-3382 10.1104/pp.103.037028
33. Rath, A.; Glibowicka, M.; Nadeau, V. G.; Chen, G.; Deber, C. M. Detergent binding explains anomalous SDS-PAGE migration of membrane proteins Proc. Natl. Acad. Sci. U. S. A. 2009, 106 (6) 1760-1765 10.1073/pnas.0813167106