Cell-free protein synthesis enables high yielding synthesis of an active multicopper oxidase

Biotechnology Journal

Volumn 11, Issue 2, 2016, Pages 212-218

  Li, Jian ^a,b,c   Lawton, Thomas J ^b   Kostecki, Jan S ^d   Nisthal, Alex ^d   Fang, Jia ^b   Mayo, Stephen L ^d   Rosenzweig, Amy C ^b,c   Jewett, Michael C ^a,b,c  

^a Northwestern University   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

^d CALIFORNIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Cell free protein synthesis; Metalloprotein; Multicopper oxidase; Transcription and translation

Indexed keywords

CELLS; COPPER; CYTOLOGY; ESCHERICHIA COLI; METAL IONS; PROTEINS; RECOMBINANT PROTEINS; SOLUBILITY; TRANSCRIPTION;

BIOTECHNOLOGICAL APPLICATIONS; CELL FREE PROTEIN SYNTHESIS; METALLOPROTEIN; MULTICOPPER OXIDASE; OXIDATIVE REACTION; RECOMBINANT HOSTS; RECOMBINANT PROTEIN EXPRESSION; TRANSLATION SYSTEMS;

BIOSYNTHESIS;

COPPER ION; COPPER SULFATE; MULTICOPPER OXIDASE; OXIDOREDUCTASE; UNCLASSIFIED DRUG; ESCHERICHIA COLI PROTEIN;

AEROMONAS HYDROPHILA; ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; BATCH REACTOR; CELL FREE PROTEIN SYNTHESIS; CELL FREE SYSTEM; CELL INCLUSION; CONTROLLED STUDY; ENZYME SYNTHESIS; ESCHERICHIA COLI; HALOARCULA MARISMORTUI; MYCELIOPHTHORA THERMOPHILA; NITROSOMONAS EUROPAEA; NONHUMAN; PRIORITY JOURNAL; PRODUCTIVITY; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SCALE UP; SCHIZOPHYLLUM COMMUNE; STREPTOMYCES COELICOLOR; BIOSYNTHESIS; CHEMISTRY; GENETIC TRANSCRIPTION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; SYNTHETIC BIOLOGY;

CELL-FREE SYSTEM; COPPER SULFATE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; OXIDOREDUCTASES; PROTEIN BIOSYNTHESIS; SYNTHETIC BIOLOGY; TRANSCRIPTION, GENETIC;

EID: 84957429636     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500030     Document Type: Article

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