Using a vector pool containing variable-strength promoters to optimize protein production in Yarrowia lipolytica

Microbial Cell Factories

Volumn 16, Issue 1, 2017, Pages

  Dulermo, Rémi ^a   Brunel, François ^a   Dulermo, Thierry ^a   Ledesma Amaro, Rodrigo ^a   Vion, Jérémy ^a   Trassaert, Marion ^a   Thomas, Stéphane ^a   Nicaud, Jean Marc ^a   Leplat, Christophe ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

Author keywords

Glucoamylase; Hybrid promoters; Protein production; RedStar2; Xylanase; Yarrowia lipolytica

Indexed keywords

AMYLASE; FUNGAL ENZYME; GLUCAN 1,4 ALPHA GLUCOSIDASE; REDSTAR2 PROTEIN; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; XYLANASE C; BACTERIAL PROTEIN; CULTURE MEDIUM; ENDO 1,4 BETA XYLANASE; FUNGAL PROTEIN; PHOTOPROTEIN; RECOMBINANT PROTEIN; YELLOW FLUORESCENT PROTEIN, BACTERIA;

ALPHA AMYLASE GENE; ARTICLE; ENZYME ACTIVITY; ENZYME SYNTHESIS; EXPRESSION VECTOR; FUNGAL GENE; FUNGUS GROWTH; GENE EXPRESSION; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; YARROWIA LIPOLYTICA; YFP GENE; BIOSYNTHESIS; CHEMISTRY; CULTURE MEDIUM; GENE VECTOR; GENETIC ENGINEERING; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES; YARROWIA;

ALPHA-AMYLASES; BACTERIAL PROTEINS; CULTURE MEDIA; ENDO-1,4-BETA XYLANASES; FUNGAL PROTEINS; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; GLUCAN 1,4-ALPHA-GLUCOSIDASE; LUMINESCENT PROTEINS; PROMOTER REGIONS, GENETIC; RECOMBINANT PROTEINS; YARROWIA;

EID: 85013096212     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/s12934-017-0647-3     Document Type: Article

References (46)

1. Domínguez A, Fermiñán E, Sánchez M, González FJ, Pérez-Campo FM, García S, Herrero AB, San Vicente A, Cabello J, Prado M, Iglesias FJ, Choupina A, Burguillo FJ, Fernández-Lago L, López MC. Non-conventional yeasts as hosts for heterologous protein production. Int Microbiol. 1998;1:131-42.
2. Madhavan A, Sukumaran RK. Promoter and signal sequence from filamentous fungus can drive recombinant protein production in the yeast Kluyveromyces lactis. Bioresour Technol. 2014;165:302-8.
3. Bragança CR, Colombo LT, Roberti AS, Alvim MC, Cardoso SA, Reis KC, de Paula SO, da Silveira WB, Passos FM. Construction of recombinant Kluyveromyces marxianus UFV-3 to express dengue virus type 1 nonstructural protein 1 (NS1). Appl Microbiol Biotechnol. 2015;99:1191-203.
4. Spohner SC, Schaum V, Quitmann H, Czermak P. Kluyveromyces lactis: an emerging tool in biotechnology. J Biotechnol. 2016;222:104-16.
5. Wagner JM, Alper HS. Synthetic biology and molecular genetics in non-conventional yeasts: current tools and future advances. Fungal Genet Biol. 2016;89:126-36.
6. Nicaud JM, Madzak C, van den Broek P, Gysler C, Duboc P, Niederberger P, Gaillardin C. Protein expression and secretion in the yeast Yarrowia lipolytica. FEMS Yeast Res. 2002;2:371-9.
7. Madzak C, Gaillardin C, Beckerich JM. Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J Biotechnol. 2004;109:63-81.
8. Nicaud JM. Yarrowia lipolytica. Yeast. 2012;29:409-18.
9. Madzak C, Beckerich JM. Heterologous protein expression and secretion in Yarrowia lipolytica. In: Yarrowia lipolytica, vol. 25. Berlin: Springer; 2013. p. 1-76.
10. Müller S, Sandal T, Kamp-Hansen P, Dalbøge H. Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Klyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast. 1998;14:1267-83.
11. Madzak C, Treton B, Blanchin-Roland S. Strong hybrid promoters and integrative expression/secretion vectors for quasiconstitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. Mol Microbiol Biotechnol. 2000;2:207-16.
12. Nthangeni MB, Urban P, Pompon D, Smit MS, Nicaud JM. The use of Yarrowia lipolytica for the expression of human cytochrome P450 CYP1A1. Yeast. 2004;21:583-92.
13. Sassi H, Delvigne F, Kar T, Nicaud JM, Coq AM, Steels S, Fickers P. Deciphering how LIP2 and POX2 promoters can optimally regulate recombinant protein production in the yeast Yarrowia lipolytica. Microb Cell Fact. 2016;15:159.
14. Gasmi N, Fudalej F, Kallel H, Nicaud JM. A molecular approach to optimize hIFN α2b expression and secretion in Yarrowia lipolytica. Appl Microbiol Biotechnol. 2011;89:109-19.
15. Barth G, Gaillardin C. Yarrowia lipolytica. In: Wolf K, editor. Non conventional yeasts in biotechnology, vol. 1. Springer: Germany; 1996. p. 313-88.
16. Chen DC, Beckerich JM, Gaillardin C. One-step transformation of the dimorphic yeast Yarrowia lipolytica. Appl Microbiol Biotechnol. 1997;48:232-5.
17. Xuan JW, Fournier P, Gaillardin C. Cloning of the LYS5 gene encoding saccharopine dehydrogenase from the yeast Yarrowia lipolytica by target integration. Curr Genet. 1988;14:15-21.
18. Le Dall MT, Nicaud JM, Gaillardin C. Multiple-copy integration in the yeast Yarrowia lipolytica. Curr Genet. 1994;26:38-44.
19. Boonvitthya N, Bozonnet S, Burapatana V, O'Donohue MJ, Chulalaksananukul W. Comparison of the heterologous expression of Trichoderma reesei endoglucanase II and cellobiohydrolase II in the yeasts Pichia pastoris and Yarrowia lipolytica. Mol Biotechnol. 2013;54:158-69.
20. Wang W, Wei H, Alahuhta M, Chen X, Hyman D, Johnson DK, Zhang M, Himmel ME. Heterologous expression of xylanase enzymes in lipogenic yeast Yarrowia lipolytica. PLoS ONE. 2014;9:e111443.
21. IN promoter. Yeast. 2012;29:59-72.
22. De Pourcq K, Vervecken W, Dewerte I, Valevska A, Van Hecke A, Callewaert N. Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man 8 GlcNAc 2 and Man5 GlcNAc2. Microb Cell Fact. 2012;11:53.
23. Lazar Z, Rossignol T, Verbeke J, Crutz-Le Coq AM, Nicaud JM, Robak M. Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications. J Ind Microbiol Biotechnol. 2013;40:1273-83.
24. Moon HY, Van TL, Cheon SA, Choo J, Kim JY, Kang HA. Cell-surface expression of Aspergillus saitoi-derived functional α-1,2-mannosidase on Yarrowia lipolytica for glycan remodeling. J Microbiol. 2013;51:506-14.
25. Nars G, Saurel O, Bordes F, Saves I, Remaud-Siméon M, André I, Milon A, Marty A. Production of stable isotope labelled lipase Lip2 from Yarrowia lipolytica for NMR: investigation of several expression systems. Protein Expr Purif. 2014;101:14-20.
26. Madzak C, Blanchin-Roland S, Cordero Otero RR, Gaillardin C. Functional analysis of upstream regulating regions from the Yarrowia lipolytica XPR2 promoter. Microbiology. 1999;145:75-87.
27. Blazeck J, Liu L, Redden H, Alper H. Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Appl Environ Microbiol. 2011;77:7905-14.
28. Blazeck J, Reed B, Garg R, Gerstner R, Pan A, Agarwala V, Alper HS. Generalizing a hybrid synthetic promoter approach in Yarrowia lipolytica. Appl Microbiol Biotechnol. 2013;97:3037-52.
29. Shabbir Hussain M, Gambill L, Smith S, Blenner MA. Engineering promoter architecture in oleaginous yeast Yarrowia lipolytica. ACS Synth Biol. 2016;5:213-23.
30. Ledesma-Amaro R, Dulermo T, Nicaud JM. Engineering Yarrowia lipolytica to produce biodiesel from raw starch. Biotechnol Biofuels. 2015;8:148.
31. Mehmood N, Husson E, Jacquard C, Wewetzer S, Büchs J, Sarazin C, Gosselin I. Impact of two ionic liquids, 1-ethyl-3-methylimidazolium acetate and 1-ethyl-3-methylimidazolium methylphosphonate, on Saccharomyces cerevisiae: metabolic, physiologic, and morphological investigations. Biotechnol Biofuels. 2015;8:17.
32. Sambrook J, Maniatis T, Fritsch EF. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press; 1989.
33. Mlícková K, Roux E, Athenstaedt K, d'Andrea S, Daum G, Chardot T, Nicaud JM. Lipid accumulation, lipid body formation, and acyl coenzyme A oxidases of the yeast Yarrowia lipolytica. Appl Environ Microbiol. 2004;70:3918-24.
34. Dulermo R, Gamboa-Meléndez H, Michely S, Thevenieau F, Neuvéglise C, Nicaud JM. The evolution of Jen3 proteins and their role in dicarboxylic acid transport in Yarrowia. Microbiologyopen. 2015;4:100-20.
35. Querol A, Barrio E, Huerta T, Ramón D. Molecular monitoring of wine fermentations conducted by active dry yeast strains. Appl Environ Microbiol. 1992;58:2948-53.
36. Dulermo R, Gamboa-Meléndez H, Ledesma-Amaro R, Thévenieau F, Nicaud JM. Unraveling fatty acid transport and activation mechanisms in Yarrowia lipolytica. Biochim Biophys Acta. 2015;1851:1202-17.
37. Viktor MJ, Rose SH, van Zyl WH, Viljoen-Bloom M. Raw starch conversion by Saccharomyces cerevisiae expressing Aspergillus tubingensis amylases. Biotechnol Biofuels. 2013;6:167.
38. Pignède G, Wang H, Fudalej F, Gaillardin C, Seman M, Nicaud JM. Autocloning vectors for gene expression and amplification for the yeast Y. lipolytica. Appl Environ Microbiol. 2000;66:3283-9.
39. Leplat C, Nicaud JM, Rossignol T. High-throughput transformation method for Yarrowia lipolytica mutant library screening. FEMS Yeast Res. 2015;15:fov052.
40. Dulermo R, Gamboa-Meléndez H, Dulermo T, Thevenieau F, Nicaud JM. The fatty acid transport protein Fat1p is involved in the export of fatty acids from lipid bodies in Yarrowia lipolytica. FEMS Yeast Res. 2014;14:883-96.
41. Idiris A, Tohda H, Kumagai H, Takegawa K. Engineering of protein secretion in yeast: strategies and impact on protein production. Appl Microbiol Biotechnol. 2010;86:403-17.
42. Li W, Zhou X, Lu P. Bottlenecks in the expression and secretion of heterologous proteins in Bacillus subtilis. Res Microbiol. 2004;155:605-10.
43. Ahmad M, Hirz M, Pichler H, Schwab H. Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production. Appl Microbiol Biotechnol. 2014;98:5301-17.
44. Liu Z, Tyo KE, Martínez JL, Petranovic D, Nielsen J. Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae. Biotechnol Bioeng. 2012;109:1259-68.
45. Greenbaum D, Colangelo C, Williams K, Gerstein M. Comparing protein abundance and mRNA expression levels on a genomic scale. Genome Biol. 2003;4:117.
46. Vogel C, Marcotte EM. Insights into the regulation of protein abundance from proteomic and transcriptomic analyses. Nat Rev Genet. 2012;13:227-32.