Optimized invertase expression and secretion cassette for improving Yarrowia lipolytica growth on sucrose for industrial applications

Journal of Industrial Microbiology and Biotechnology

Volumn 40, Issue 11, 2013, Pages 1273-1283

  Lazar, Zbigniew ^a,d   Rossignol, Tristan ^b   Verbeke, Jonathan ^b   Crutz Le Coq, Anne Marie ^b   Nicaud, Jean Marc ^b,c   Robak, Małgorzata ^a  

^a WROC AW UNIVERSITY OF ENVIRONMENTAL AND LIFE SCIENCES   (Poland)

^b UMR1319 MICALIS   (France)

^c CNRS   (France)

^d AGROPARISTECH   (France)

Author keywords

Citric acid; Invertase; Secretion; Sucrose; Yarrowia lipolytica

Indexed keywords

BETA FRUCTOFURANOSIDASE; CITRIC ACID; SUCROSE;

ARTICLE; BIOMASS; BIOREACTOR; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SYNTHESIS; FUNGAL CELL; FUNGUS GROWTH; GENE CASSETTE; GROWTH RATE; HEXOSE TRANSPORT; NONHUMAN; PLASMID; PROMOTER REGION; PROTEIN EXPRESSION; RESTRICTION SITE; SACCHAROMYCES CEREVISIAE; SUGAR INTAKE; YARROWIA LIPOLYTICA;

SACCHAROMYCES CEREVISIAE; YARROWIA LIPOLYTICA;

BETA-FRUCTOFURANOSIDASE; BIOMASS; BIOREACTORS; BIOTECHNOLOGY; CITRIC ACID; CULTURE MEDIA; FRUCTOSE; GLUCOSE; HYDROLYSIS; PROMOTER REGIONS, GENETIC; REPRODUCIBILITY OF RESULTS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SUCROSE; YARROWIA;

EID: 84886314772     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-013-1323-1     Document Type: Article

References (38)

1. Ageitos J, Vallejo J, Veiga-Crespo P, Villa T (2011) Oily yeasts as oleaginous cell factories. Appl Microbiol Biotechnol 90(4):1219-1227. doi: 10.1007/s00253-011-3200-z
2. Barth G, Gaillardin C (1996) The dimorphic fungus Yarrowia lipolytica. In: Wolf K (ed) Genetics, biochemistry and molecular biology of non-conventional yeasts. Springer, Berlin Heidelberg New York, pp 313-388
3. Beopoulos A, Chardot T, Nicaud JM (2009) Yarrowia lipolytica: a model and a tool to understand the mechanisms implicated in lipid accumulation. Biochimie 91(6):692-696
4. Blazeck J, Liu L, Redden H, Alper H (2011) Tuning gene expression in Yarrowia lipolytica using a hybrid promoter approach. Appl Environ Microbiol 77(22):7905-7914. doi: 10.1128/aem.05763-11
5. Bordes F, Fudalej F, Dossat V, Nicaud JM, Marty A (2007) A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica. J Microbiol Methods 70(3):493-502
6. Carlson M, Taussig R, Kustu S, Botstein D (1983) The secreted form of invertase in Saccharomyces cerevisiae is synthesized from mRNA encoding a signal sequence. Mol Cell Biol 3(3):439-447
7. 2 N-glycan core. PLoS One 7(6):e39976
8. Fickers P, Le Dall MT, Gaillardin C, Thonart P, Nicaud JM (2003) New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica. J Microbiol Methods 55(3):727-737
9. Förster A, Aurich A, Mauersberger S, Barth G (2007) Citric acid production from sucrose using a recombinant strain of the yeast Yarrowia lipolytica. Appl Microbiol Biotechnol 75(6):1409-1417. doi: 10.1007/s00253-007- 0958-0
10. Gaillardin C, Ribet AM, Heslot H (1985) Integrative transformation of the yeast Yarrowia lipolytica. Curr Genet 10(1):49-58. doi: 10.1007/bf00418493
11. Gasmi N, Fudalej F, Kallel H, Nicaud JM (2011) A molecular approach to optimize hIFN a2b expression and secretion in Yarrowia lipolytica. Appl Microbiol Biotechnol 89(1):109-119. doi: 10.1007/s00253-010-2803-0
12. Goldberg DM, Ellis G (1983) Isocitrate dehydrogenase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 3. Verlag Chemie, Weinheim, pp 183-190
13. Haddouche R, Poirier Y, Delessert S, Sabirova J, Pagot Y, Neuvéglise C, Nicaud J-M (2011) Engineering polyhydroxyalkanoate content and monomer composition in the oleaginous yeast Yarrowia lipolytica by modifying the ß-oxidation multifunctional protein. Appl Microbiol Biotechnol 91(5):1327-1340. doi: 10.1007/s00253-011-3331-2
14. Hong S-P, Seip J, Walters-Pollak D, Rupert R, Jackson R, Xue Z, Zhu Q (2012) Engineering Yarrowia lipolytica to express secretory invertase with strong FBA1IN promoter. Yeast 29(2):59-72. doi: 10.1002/yea.1917
15. Kabran P, Rossignol T, Gaillardin C, Nicaud JM, Neuvéglise C (2012) Alternative splicing regulates targeting of malate dehydrogenase in Yarrowia lipolytica. DNA Res 19(3):231-244. doi: 10.1093/dnares/dss007
16. Kotwal SM, Shankar V (2009) Immobilized invertase. Biotechnol Adv 27(4):311-322
17. Lazar Z, Walczak E, Robak M (2011) Simultaneous production of citric acid and invertase by Yarrowia lipolytica SUC+ transformants. Bioresour Technol 102(13):6982-6989
18. Li Q, Du W, Liu D (2008) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80(5):749-756. doi: 10.1007/s00253-008- 1625-9
19. Madzak C, Gaillardin C, Beckerich JM (2004) Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J Biotechnol 109(1-2):63-81
20. Madzak C, Treton B, Blanchin-Roland S (2000) Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. J Mol Microbiol Biotechnol 2(2):207-216
21. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31(3):426-428. doi: 10.1021/ac60147a030
22. Moeller L, Zehnsdorf A, Aurich A, Bley T, Strehlitz B (2012) Substrate utilization by recombinant Yarrowia lipolytica growing on sucrose. Appl Microbiol Biotechnol 93(4):1695-1702. doi: 10.1007/s00253-011-3681-9
23. Müller S, Sandal T, Kamp-Hansen P, Dalbøge H (1998) Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Kluyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 14 (14):1267-1283. doi: 10.1002/(sici)1097-0061(1998100)14:14<1267:: aid-yea327>3.0.co;2-2
24. Nicaud JM (2012) Yarrowia lipolytica. Yeast 29(10):409-418. doi: 10.1002/yea.2921
25. Nicaud JM, Fabre E, Gaillardin C (1989) Expression of invertase activity in Yarrowia lipolytica and its use as a selective marker. Curr Genet 16(4):253-260
26. Ogrydziak DM, Nicaud JM (2012) Characterization of Yarrowia lipolytica XPR2 multi-copy strains over-producing alkaline extracellular protease - a system for rapidly increasing secretory pathway cargo loads. FEMS Yeast Res 12(8):938-948. doi: 10.1111/j.1567-1364.2012.00846.x
27. Papanikolaou S, Chatzifragkou A, Fakas S, Galiotou-Panayotou M, Komaitis M, Nicaud J-M, Aggelis G (2009) Biosynthesis of lipids and organic acids by Yarrowia lipolytica strains cultivated on glucose. Eur J Lipid Sci Technol 111(12):1221-1232. doi: 10.1002/ejlt.200900055
28. Papanikolaou S, Galiotou-Panayotou M, Chevalot I, Komaitis M, Marc I, Aggelis G (2006) Influence of glucose and saturated free-fatty acid mixtures on citric acid and lipid production by Yarrowia lipolytica. Curr Microbiol 52(2):134-142. doi: 10.1007/s00284-005-0223-7
29. Rymowicz W, Rywinska A, Marcinkiewicz M (2009) High-yield production of erythritol from raw glycerol in fed-batch cultures of Yarrowia lipolytica. Biotechnol Lett 31(3):377-380. doi: 10.1007/s10529-008-9884-1
30. Rywinska A, Juszczyk P, Wojtatowicz M, Robak M, Lazar Z, Tomaszewska L, Rymowicz W (2013) Glycerol as a promising substrate for Yarrowia lipolytica biotechnological applications. Biomass Bioenergy 48:148-166. doi: 10.1016/j.biombioe.2012.11.021
31. Rywinska A, Rymowicz W, Zarowska B, Skrzypinski A (2010) Comparison of citric acid production from glycerol and glucose by different strains of Yarrowia lipolytica. World J Microbiol Biotechnol 26(7):1217-1224. doi: 10.1007/s11274-009-0291-0
32. Sniezewski L, Walczak E, Lazar Z, Robak M (2012) Low peptone dose as inductor of alkaline protease promoter used for invertase gene expression in Yarrowia lipolytica. J Life Sci 6:1100-1108
33. Tomaszewska L, Rywińska A, Gładkowski W (2012) Production of erythritol and mannitol by Yarrowia lipolytica yeast in media containing glycerol. J Indust Microbiol Biotechnol 1-11. doi: 10.1007/s10295-012-1145-6
34. Turki S, Ayed A, Chalghoumi Nj, Weekers F, Thonart P, Hl Kallel (2010) An enhanced process for the production of a highly purified extracellular lipase in the non-conventional yeast Yarrowia lipolytica. Appl Biochem Biotechnol 160(5):1371-1385. doi: 10.1007/s12010-009-8599-7
35. Walczak E, Robak M (2009) Growth on sucrose of Yarrowia lipolytica yeasts clones with invertase gene from Saccharomyces cerevisiae [in Polish]. Acta Sci Pol Biotechnol 8 (4):25-36. http://www.acta.media.pl/pl/full/3/2009/ 000030200900008000040002500036.pdf
36. Wojtatowicz M, Rymowicz W (1991) Effect of inoculum on kinetics and yield of citric acid production on glucose media by Yarrowia lipolytica A-101. Acta Alimentaria Polonica 41(2):137-143
37. Wojtatowicz M, Rymowicz W, Robak M, Zarowska B, Nicaud JM (1997) Kinetics of cell growth and citric acid production by Yarrowia lipolytica Suc+ transformants in sucrose media. Pol J Food Nutr Sci 6(4):49-54
38. Zhang B, Rong C, Chen H, Song Y, Zhang H, Chen W (2012) De novo synthesis of trans-10, cis-12 conjugated linoleic acid in oleaginous yeast Yarrowia lipolytica. Microb Cell Fact 11(1):51