A molecular approach to optimize hIFN-α2b expression and secretion in Yarrowia lipolytica

Applied Microbiology and Biotechnology

Volumn 89, Issue 1, 2011, Pages 109-119

  Gasmi, Najla ^a,b   Fudalej, Franck ^a,c   Kallel, Héla ^b   Nicaud, Jean Marc ^a,d  

^a UMR1319 MICALIS   (France)

^b PASTEUR INSTITUTE OF TUNIS   (Tunisia)

^c Oxyrane Belgium NV   (Belgium)

^d CNRS   (France)

Author keywords

Codon optimization; Heterologous expression; hIFN 2b; Targeting sequence; Yarrowia lipolytica

Indexed keywords

CODON BIAS; CODON OPTIMIZATION; CODON USAGE; CONSENSUS SEQUENCE; EXPRESSION LEVELS; EXTRACELLULAR LIPASE; EXTRACELLULAR SECRETION; HETEROLOGOUS EXPRESSION; HETEROLOGOUS PROTEINS; INDUCIBLE PROMOTER; MOLECULAR APPROACH; PRODUCTION LEVEL; PROTEIN PRODUCTION; PROTEIN SECRETION; TARGET PROTEINS; TARGETING SEQUENCE; TRANSLATION INITIATION SITE; TRANSLATIONAL EFFICIENCIES; WILD TYPES; Y. LIPOLYTICA; YARROWIA LIPOLYTICA;

GENES; PROTEINS;

OPTIMIZATION;

ACYL COENZYME A OXIDASE; ALPHA2B INTERFERON;

ENZYME ACTIVITY; MOLECULAR ANALYSIS; OPTIMIZATION; PROTEIN; SECRETION; YEAST;

ARTICLE; CODON; CONSENSUS SEQUENCE; CONTROLLED STUDY; GENE SEQUENCE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SECRETION; TRANSLATION INITIATION; YARROWIA LIPOLYTICA;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; CODON; EXTRACELLULAR SPACE; GENE EXPRESSION; HUMANS; INTERFERON ALFA-2B; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEIN SORTING SIGNALS; PROTEIN TRANSPORT; YARROWIA;

YARROWIA LIPOLYTICA;

EID: 78651083665     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2803-0     Document Type: Article

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