Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man8GlcNAc2 and Man5GlcNAc2

Microbial Cell Factories

Volumn 11, Issue , 2012, Pages

  De Pourcq, Karen ^a,b   Vervecken, Wouter ^a,b,c   Dewerte, Isabelle ^a,b   Valevska, Albena ^a,b,c   Van Hecke, Annelies ^a,b   Callewaert, Nico ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

^c Oxyrane Belgium   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; GENOMIC DNA; GLUCOSYLCERAMIDASE; GLYCOSYLATED PROTEIN; MANNOSE; MANNOSYLTRANSFERASE;

AMINO ACID SEQUENCE; ARTICLE; BIOACCUMULATION; BIOTRANSFORMATION; CODON; ENZYME INACTIVATION; ESCHERICHIA COLI; FUNGAL STRAIN; GENE AMPLIFICATION; HYPOCREA JECORINA; IN VITRO STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; PROTEIN SYNTHESIS; RECOMBINANT PLASMID; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS; SIGNAL TRANSDUCTION; YARROWIA LIPOLYTICA;

CARBOHYDRATE SEQUENCE; FUNGAL PROTEINS; GENETIC ENGINEERING; GLUCOSYLCERAMIDASE; GLYCOPROTEINS; GLYCOSYLATION; HUMANS; MANNOSE; MANNOSIDASES; MANNOSYLTRANSFERASES; MOLECULAR SEQUENCE DATA; POLYSACCHARIDES; RECOMBINANT PROTEINS; TRICHODERMA; YARROWIA;

YARROWIA LIPOLYTICA;

EID: 84862183483     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-11-53     Document Type: Article

References (30)

1. Kogelberg H, Tolner B, Sharma SK, Lowdell MW, Qureshi U, Robson M, Hillyer T, Pedley RB, Vervecken W, Contreras R, Begent RHJ, Chester KA. Clearance mechanism of a mannosylated antibody-enzyme fusion protein used in experimental cancer therapy. Glycobiology 2006, 17:36-45. 10.1093/glycob/cwl053, 17000699.
2. Doebber TW, Wu MS, Bugianesi RL, Ponpipom MM, Furbish FS, Barranger JA, Brady RO, Shen TY. Enhanced macrophage uptake of synthetically glycosylated human placental beta-glucocerebrosidase. J Biol Chem 1982, 257:2193-2199.
3. Murray GJ. Lectin-specific targeting of lysosomal enzymes to reticuloendothelial cells. Methods Enzymol 1987, 149:25-42.
4. Barton NW, Brady RO, Dambrosia JM, Di Bisceglie AM, Doppelt SH, Hill SC, Mankin HJ, Murray GJ, Parker RI, Argoff CE, Grewal RP, Yu K-T, Graham OC, Holder CA, Howard KD, Kaneski CR, Oliver KL, Riesz S, Verderese CL, Zirzow GC. Replacement therapy for inherited enzyme deficiency-macrophage-targeted glucocerebrosidase for Gaucher's disease. N Engl J Med 1991, 324:1464-1470. 10.1056/NEJM199105233242104, 2023606.
5. Sato Y, Beutler E. Binding, internalization, and degradation of mannose-terminated glucocerebrosidase by macrophages. J Clin Invest 1993, 91:1909-1917. 10.1172/JCI116409, 288185, 8486762.
6. Madzak C, Gaillardin C, Beckerich JM. Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review. J Biotechnol 2004, 109:63-81. 10.1016/j.jbiotec.2003.10.027, 15063615.
7. Nakayama K, Nagasu T, Shimma Y, Kuromitsu J, Jigami Y. OCH1 encodes a novel membrane bound mannosyltransferase: outer chain elongation of asparagine-linked oligosaccharides. EMBO J 1992, 11:2511-2519. 556726, 1628616.
8. Jungmann J, Munro S. Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with α-1,6-mannosyltransferase activity. EMBO J 1998, 17:423-434. 10.1093/emboj/17.2.423, 1170393, 9430634.
9. De Pourcq K, De Schutter K, Callewaert N. Engineering of glycosylation in yeast and other fungi: current state and perspectives. Appl Microbiol Biotechnol 2010, 87:1617-1631. 10.1007/s00253-010-2721-1, 20585772.
10. Barnay-Verdier S, Boisrame A, Beckerich JM. Identification and characterization of two α-1,6-mannosyltransferases, Anl1p and Och1p, in the yeast Yarrowia lipolytica. Microbiology 2004, 150:2185-2195. 10.1099/mic.0.26887-0, 15256561.
11. Song Y, Choi MH, Park JN, Kim MW, Kim EJ, Kang HA, Kim JY. Engineering of the yeast Yarrowia lipolytica for the production of glycoproteins lacking the outer-chain mannose residues of N-glycans. Appl Environ Microbiol 2007, 73:4446-4454. 10.1128/AEM.02058-06, 1932813, 17513593.
12. Stolz J, Munro S. The components of the Saccharomyces cerevisiae mannosyl-transferase complex M-Pol I have distinct functions in mannan synthesis. J Biol Chem 2002, 277:44801-44808. 10.1074/jbc.M208023200, 12235155.
13. Nakanishi-Shindo Y, Nakayama K, Tanaka A, Toda Y, Jigami Y. Structure of the N-linked oligosaccharides that show the complete loss of α-1,6-polymannose outer chain from och1, och1 mnn1, and och1 mnn1alg3 mutants of Saccharomyces cerevisiae. J Biol Chem 1993, 268:26338-26345.
14. Yoko-o T, Tsukahara K, Watanabe T, Hata-Sugi N, Yoshimatsu K, Nagasu T, Jigami Y. Schizosaccharomyces pombe och1+ encodes α-1,6-mannosyltransferase that is involved in outer chain elongation of N-linked oligosaccharides. FEBS Lett 2001, 489:75-80. 10.1016/S0014-5793(01)02082-8, 11231017.
15. Vervecken W, Kaigorodov V, Callewaert N, Geysens S, De Vusser K, Contreras R. In vivo synthesis of mammalian-like, hybrid-type N-glycans in Pichia pastoris. Appl Environ Microbiol 2004, 70:2639-2646. 10.1128/AEM.70.5.2639-2646.2004, 404441, 15128513.
16. Kim MW, Kim EJ, Kim JY, Park JS, Oh DB, Shimma Y, Chiba Y, Jigami Y, Rhee SK, Kang HA. Functional characterization of the Hansenula polymorpha HOC1, OCH1, and OCR1 genes as members of the yeast OCH1 mannosyltransferase family involved in protein glycosylation. J Biol Chem 2006, 281:6261-6272. 10.1074/jbc.M508507200, 16407250.
17. Alessandrini F, Pfister S, Kremmer E, Gerber JK, Ring J, Behrendt H. Alterations of glucosylceramide-beta-glucosidase levels in the skin of patients with psoriasis vulgaris. J Invest Dermatol 2004, 123:1030-1036. 10.1111/j.0022-202X.2004.23469.x, 15610510.
18. Callewaert N, Laroy W, Cadirgi H, Geysens S, Saelens X, Min Jou W, Contreras R. Use of HDEL-tagged Trichoderma reesei mannosyl oligosaccharide 1,2-α-D-mannosidase for N-glycan engineering in Pichia pastoris. FEBS Lett 2001, 503:173-178. 10.1016/S0014-5793(01)02676-X, 11513877.
19. Madzak C, Treton B, Blanchin-Roland S. Strong hybrid promoters and integrative expression/secretion vectors for quasi-constitutive expression of heterologous proteins in the yeast Yarrowia lipolytica. J Mol Microbiol Biotechnol 2000, 2:207-216.
20. Romero PA, Sleno B, Herscovics A. Glycoprotein biosynthesis in Saccharomyces cerevisiae. Partial purification of the α-1,6-mannosyltransferase that initiates outer chain synthesis. Glycobiology 1994, 4:135-140. 10.1093/glycob/4.2.135, 8054713.
21. Nakayama K, Nakanishi-Shindo Y, Tanaka A, Haga-Toda Y, Jigami Y. Substrate specificity of α-1,6-mannosyltransferase that initiates N-linked mannose outer chain elongation in Saccharomyces cerevisiae. FEBS Lett 1997, 412:547-550. 10.1016/S0014-5793(97)00634-0, 9276464.
22. Gasmi N, Fudalej F, Kallel H, Nicaud JM. A molecular approach to optimize hIFN α2b expression and secretion in Yarrowia lipolytica. Appl Microbiol Biotechnol 2011, 89:109-119. 10.1007/s00253-010-2803-0, 20821205.
23. Fickers P, Fudalej F, Le Dall MT, Casaregola S, Gaillardin C, Thonart P, Nicaud JM. Identification and characterisation of LIP7 and LIP8 genes encoding two extracellular triacylglycerol lipases in the yeast Yarrowia lipolytica. Fungal Genet Biol 2005, 42:264-274. 10.1016/j.fgb.2004.12.003, 15707847.
24. Barth G, Gaillardin C. Yarrowia lipolytica. Nonconventional Yeasts in Biotechnology. A Handbook 1996, 313-388. Berlin: Springer, Wolf K.
25. Barth G, Gaillardin C. Physiology and genetics of the dimorphic fungus Yarrowia lipolytica. FEMS Microbiol Rev 1997, 19:219-237. 10.1111/j.1574-6976.1997.tb00299.x, 9167256.
26. Fickers P, Le Dall MT, Gaillardin C, Thonart P, Nicaud JM. New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica. J Microbiol Methods 2003, 55:727-737. 10.1016/j.mimet.2003.07.003, 14607415.
27. Vervecken W, Callewaert N, Kaigorodov V, Geysens S, Contreras R. Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids. Methods Mol Biol 2007, 389:119-138. 10.1007/978-1-59745-456-8_9, 17951639.
28. Nicaud JM, Madzak C, van den Broek P, Gysler C, Duboc P, Niederberger P, Gaillardin C. Protein expression and secretion in the yeast Yarrowia lipolytica. FEMS Yeast Res 2002, 2:371-379.
29. Ballou L, Hernandez LM, Alvarado E, Ballou CE. Revision of the oligosaccharide structures of yeast carboxypeptidase Y. Proc Natl Acad Sci USA 1990, 87:3368-3372. 10.1073/pnas.87.9.3368, 53901, 2185468.
30. Laroy W, Contreras R, Callewaert N. Glycome mapping on DNA sequencing equipment. Nat Protoc 2006, 1:397-405. 10.1038/nprot.2006.60, 17406262.