Construction of recombinant Kluyveromyces marxianus UFV-3 to express dengue virus type 1 nonstructural protein 1 (NS1)

Applied Microbiology and Biotechnology

Volumn 99, Issue 3, 2015, Pages 1191-1203

  Bragança, Caio Roberto Soares ^a   Colombo, Lívia Tavares ^a   Roberti, Alvaro Soares ^a   Alvim, Mariana Caroline Tocantins ^a   Cardoso, Silvia Almeida ^a   Reis, Kledna Constancio Portes ^b   de Paula, Sérgio Oliveira ^a   da Silveira, Wendel Batista ^a   Passos, Flavia Maria Lopes ^a  

^a FEDERAL UNIVERSITY OF VIÇOSA   (Brazil)

^b Microbiologia Veterinária Especial LTDA Microvet   (Brazil)

Author keywords

Dengue virus; Heterologous expression; Kluyveromyces marxianus; NS1 protein

Indexed keywords

CLONING; COENZYMES; SODIUM DODECYL SULFATE; SULFUR COMPOUNDS; TRANSCRIPTION; VIRUSES; YEAST;

DENGUE VIRUS; DENGUE VIRUS PROTEINS; DIAGNOSTIC APPLICATIONS; EXPRESSION OF HETEROLOGOUS PROTEINS; HETEROLOGOUS EXPRESSION; HIGH LEVEL EXPRESSION; KLUYVEROMYCES MARXIANUS; NONSTRUCTURAL PROTEINS;

RECOMBINANT PROTEINS;

NS1 PROTEIN, DENGUE-1 VIRUS; RECOMBINANT PROTEIN; VIRUS PROTEIN;

DNA SEQUENCE; GENE EXPRESSION; GENE VECTOR; GENETIC RECOMBINATION; GENETIC TRANSFORMATION; GENETICS; IMMUNOBLOTTING; KLUYVEROMYCES; METABOLISM; MOLECULAR GENETICS; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TRANSCRIPTION INITIATION;

ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION; GENETIC VECTORS; IMMUNOBLOTTING; KLUYVEROMYCES; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS, DNA; TRANSCRIPTIONAL ACTIVATION; TRANSFORMATION, GENETIC; VIRAL NONSTRUCTURAL PROTEINS;

EID: 84922000508     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-014-5963-5     Document Type: Article

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