Development of a recombinant Escherichia coli strain for overproduction of the plant pigment anthocyanin

Applied and Environmental Microbiology

Volumn 81, Issue 18, 2015, Pages 6276-6284

  Lim, Chin Giaw ^a   Wong, Lynn ^a,b   Bhan, Namita ^b   Dvora, Hila ^a   Xu, Peng ^a,b   Venkiteswaran, Sankaranarayanan ^a   Koffas, Mattheos A G ^a,b  

^a State University of New York   (United States)

^b RENSSELAER POLYTECHNIC INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COST EFFECTIVENESS; ESCHERICHIA COLI; FLAVONOIDS; GENE EXPRESSION; MICROORGANISMS; ORGANIC COMPOUNDS; PHENOLS;

ANTHOCYANIDIN SYNTHASE; ANTI-AGING PROPERTIES; COLORIMETRIC ASSAYS; GENE EXPRESSION LEVELS; LARGE SCALE PRODUCTIONS; RECOMBINANT ESCHERICHIA COLI; RECOMBINANT MICROORGANISM; TRANSPORTER PROTEINS;

ANTHOCYANINS;

BACTERIUM; BIOASSAY; BIOENGINEERING; GENE EXPRESSION; PIGMENT; PROTEIN; SECRETION;

ESCHERICHIA COLI;

ANTHOCYANIN; CATECHIN; FOOD DYE; PIGMENT;

BIOSYNTHESIS; BIOTRANSFORMATION; COLORIMETRY; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION; GENETICS; METABOLIC ENGINEERING; METABOLISM; PROCEDURES;

ANTHOCYANINS; BIOSYNTHETIC PATHWAYS; BIOTRANSFORMATION; CATECHIN; COLORIMETRY; ESCHERICHIA COLI; FERMENTATION; FOOD COLORING AGENTS; GENE EXPRESSION; METABOLIC ENGINEERING; PIGMENTS, BIOLOGICAL;

EID: 84945262305     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01448-15     Document Type: Article

References (46)

1. Xu P, Bhan N, Koffas MA. 2013. Engineering plant metabolism into microbes: from systems biology to synthetic biology. Curr Opin Biotechnol 24:291-299. http://dx.doi.org/10.1016/j.copbio.2012.08.010.
2. Bhan N, Xu P, Koffas MAG. 2013. Pathway and protein engineering approaches to produce novel and commodity small molecules. Curr Opin Biotechnol 24:1137-1143. http://dx.doi.org/10.1016/j.copbio.2013.02.019.
3. Mora-Pale M, Sanchez-Rodriguez SP, Linhatrdt RJ, Dordick JS, Koffas MAG. 2013. Metabolic engineering and in vitro biosynthesis of phytochemicals and non-natural analogues. Plant Sci 210:10-24. http://dx.doi .org/10.1016/j.plantsci.2013.05.005.
4. Mora-Pale M, Sanchez-Rodriguez SP, Linhardt RJ, Dordick JS, Koffas MAG. 2014. Biochemical strategies for enhancing the in vivo production of natural products with pharmaceutical potential. Curr Opin Biotechnol 25:86-94. http://dx.doi.org/10.1016/j.copbio.2013.09.009.
5. Lin Y, Jain R, Yan Y. 2014. Microbial production of antioxidant food ingredients via metabolic engineering. Curr Opin Biotechnol 26:71-78. http://dx.doi.org/10.1016/j.copbio.2013.10.004.
6. Lin Y, Yan Y. 2014. Biotechnological production of plant-specific hydroxylated phenylpropanoids. Biotechnol Bioeng 111:1895-1899. http: //dx.doi.org/10.1002/bit.25237.
7. Chemler JA, Koffas MA. 2008. Metabolic engineering for plant natural product biosynthesis in microbes. Curr Opin Biotechnol 19:597-605. http://dx.doi.org/10.1016/j.copbio.2008.10.011.
8. Nielsen J, Jewett MC. 2008. Impact of systems biology on metabolic engineering of Saccharomyces cerevisiae. FEMS Yeast Res 8:122-131. http://dx.doi.org/10.1111/j.1567-1364.2007.00302.x.
9. Nielsen J, Larsson C, van Maris A, Pronk J. 2013. Metabolic engineering of yeast for production of fuels and chemicals. Curr Opin Biotechnol 24:398-404. http://dx.doi.org/10.1016/j.copbio.2013.03.023.
10. Förster J, Famili I, Fu P, Palsson B, Nielsen J. 2003. Genome-scale reconstruction of the Saccharomyces cerevisiae metabolic network. Genome Res 13:244-253. http://dx.doi.org/10.1101/gr.234503.
11. Bro C, Regenberg B, Förster J, Nielsen J. 2006. In silico aided metabolic engineering of Saccharomyces cerevisiae for improved bioethanol production. Metab Eng 8:102-111. http://dx.doi.org/10.1016/j.ymben.2005 .09.007.
12. Ververidis F, Trantas E, Douglas C, Vollmer G, Kretzschmar G, Panopoulos N. 2007. Biotechnology of flavonoids and other phenylpropanoidderived natural products. Part II: reconstruction of multienzyme pathways in plants and microbes. Biotechnol J 2:1235-1249.
13. Willrodt C, Karande R, Schmid A, Jusling M. 2015. Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants. Curr Opin Biotechnol 35:52-62. http://dx.doi.org/10 .1016/j.copbio.2015.03.010.
14. Mateus N, De Freitas V. 2008. Anthocyanins as food colorants, p 283-304. In Gould K, Davies KM, Winefield C (ed), Anthocyanins: biosynthesis, functions, and applications. Springer, New York, N.Y.
15. McCann D, Barrett A, Cooper A, Crumpler D, Dalen L, Grimshaw K, Kitchin E, Lok K, Porteous L, Prince E, Sonuga-Barke E, Warner JO, Stevenson J. 2007. Food additives and hyperactive behaviour in 3-yearold and 8/9-year-old children in the community: a randomised, doubleblinded, placebo-controlled trial. Lancet 370:1560-1567. http://dx.doi .org/10.1016/S0140-6736(07)61306-3.
16. Stevens LJ, Kuczek T, Burgess JR, Stochelski MA, Arnold LE, Galland L. 2013. Mechanisms of behavioral, atopic, and other reactions to artificial food colors in children. Nutr Rev 71:268-281. http://dx.doi.org/10.1111 /nure.12023.
17. Espín JC, García-Conesa MT, Tomás-Barberán FA. 2007. Nutraceuticals: facts and fiction. Phytochemistry 68:2986-3008. http://dx.doi.org/10 .1016/j.phytochem.2007.09.014.
18. Santos-Buelga C, Mateus N, De Freitas V. 2014. Anthocyanins. Plant pigments and beyond. J Agric Food Chem 62:6879-6884.
19. Pietta PG. 2000. Flavonoids as antioxidants. J Nat Prod 63:1035-1042. http://dx.doi.org/10.1021/np9904509.
20. Yan Y, Chemler J, Huang L, Martens S, Koffas MA. 2005. Metabolic engineering of anthocyanin biosynthesis in Escherichia coli. Appl Environ Microbiol 71:3617-3623. http://dx.doi.org/10.1128/AEM.71.7.3617-3623 .2005.
21. Yan Y, Li Z, Koffas MA. 2008. High-yield anthocyanin biosynthesis in engineered Escherichia coli. Biotechnol Bioeng 100:126-140. http://dx .doi.org/10.1002/bit.21721.
22. Leonard E, Yan Y, Fowler ZL, Li Z, Lim CG, Lim KH, Koffas MA. 2008. Strain improvement of recombinant Escherichia coli for efficient production of plant flavonoids. Mol Pharm 5:257-265. http://dx.doi.org/10.1021 /mp7001472.
23. Zhao S, Jones JA, Lachance D, Bhan N, Khalidi O, Venkataraman S, Wang Z, Koffas MAG. 2015. Improvement of catechin production in Escherichia coli through combinatorial metabolic engineering. Metab Eng 28:43-53. http://dx.doi.org/10.1016/j.ymben.2014.12.002.
24. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, New York, N.Y.
25. Datsenko KA, Wanner BL. 2000. One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640 - 6645. http://dx.doi.org/10.1073/pnas.120163297.
26. Nakajima J, Tanaka Y, Yamazaki M, Saito K. 2001. Reaction mechanism from leucoanthocyanidin to anthocyanidin 3-glucoside, a key reaction for coloring in anthocyanin biosynthesis. J Biol Chem 276:25797-25803. http: //dx.doi.org/10.1074/jbc.M100744200.
27. Zhang H, Wang L, Deroles S, Bennett R, Davies K. 2006. New insight into the structures and formation of anthocyanic vacuolar inclusions in flower petals. BMC Plant Biol 6:29-35. http://dx.doi.org/10.1186/1471 -2229-6-29.
28. Springob K, Nakajima J, Yamazaki M, Saito K. 2003. Recent advances in the biosynthesis and accumulation of anthocyanins. Nat Prod Rep 20: 288-303. http://dx.doi.org/10.1039/b109542k.
29. Piddock LJV. 2006. Clinically relevant chromosomally encoded multidrug resistance efflux pumps in bacteria. Clin Microbiol Rev 19:382-402. http://dx.doi.org/10.1128/CMR.19.2.382-402.2006.
30. Burse A, Weingart H, Ullrich MS. 2004. The phytoalexin-inducible multidrug efflux pump AcrAB contributes to virulence in the fire blight pathogen, Erwinia amylovora. Mol Plant Microbe Interact 17:43-54. http: //dx.doi.org/10.1094/MPMI.2004.17.1.43.
31. Fujii T, Fujii Y, Machida K, Ochiai A, Ito M. 2009. Efficient biotransformations using Escherichia coli with tolC acrAB mutations expressing cytochrome P450 genes. Biosci Biotechnol Biochem 73:805-810. http: //dx.doi.org/10.1271/bbb.80627.
32. Lim HN, Lee Y, Hussein R. 2011. Fundamental relationship between operon organization and gene expression. Proc Natl Acad Sci U S A 108: 10626-10631. http://dx.doi.org/10.1073/pnas.1105692108.
33. Xu P, Wang W, Li L, Bhan N, Zhang F, Koffas MAG. 2014. Design and kinetic analysis of a hybrid promoter-regulator system for malonyl-CoA sensing in Escherichia coli. ACS Chem Biol 9:451-458. http://dx.doi.org /10.1021/cb400623m.
34. Xu P, Li L, Zhang F, Stephanopoulos G, Koffas M. 2014. Improving fatty acids production by engineering dynamic pathway regulation and metabolic control. Proc Natl Acad Sci U S A 111:11299-11304. http://dx.doi .org/10.1073/pnas.1406401111.
35. Kuznetsova E, Proudfoot M, Gonzalez CF, Brown G, Omelchenko MV, Borozan I, Carmel L, Wolf YI, Mori H, Savchenko AV, Arrowsmith CH, Koonin EV, Edwards AM, Yakunin AF. 2006. Genome-wide analysis of substrate specificities of the Escherichia coli haloacid dehalogenaselike phosphatase family. J Biol Chem 281:36149-36161. http://dx.doi.org /10.1074/jbc.M605449200.
36. Dietrich JA, McKee AE, Keasling JD. 2010. High-throughput metabolic engineering: advances in small-molecule screening and selection. Annu Rev Biochem 79:563-590. http://dx.doi.org/10.1146/annurev-biochem -062608-095938.
37. Cress BF, Toparlak OD, Guleria S, Lebovich M, Stieglitz JT, Englaender JA, Jones JA, Linhardt RJ, Koffas MA. 30 March 2015. CRISPathBrick: modular combinatorial assembly of type II-A CRISPR arrays for dCas9- mediated multiplex transcriptional repression in E. coli. ACS Synth Biol http://dx.doi.org/10.1021/acssynbio.5b00012.
38. Jones JA, Toparlak OD, Koffas MA. 2015. Metabolic pathway balancing and its role in the production of biofuels and chemicals. Curr Opin Biotechnol 33:52-59.
39. Novy R, Morris B. 2001. Use of glucose to control basal expression in the pET system. Innovations 13:13-15.
40. Castañeda-Ovando A, de Lourdes Pacheco-Hernández M, Páez- Hernández ME, Rodríguez JA, Galán-Vidal CA. 2009. Chemical studies of anthocyanins: a review. Food Chem 113:859-871. http://dx.doi.org/10 .1016/j.foodchem.2008.09.001.
41. Santos CNS, Stephanopoulos G. 2008. Melanin-based high-throughput screen for L-tyrosine production in Escherichia coli. Appl Environ Microbiol 74:1190-1197. http://dx.doi.org/10.1128/AEM.02448-07.
42. Lim CG, Fowler ZL, Hueller T, Schaffer S, Koffas MA. 2011. High-yield resveratrol production in engineered Escherichia coli. Appl Environ Microbiol 77:3451-3460. http://dx.doi.org/10.1128/AEM.02186-10.
43. Xu P, Ranganathan S, Fowler ZL, Maranas CD, Koffas MA. 2011. Genome-scale metabolic network modeling results in minimal interventions that cooperatively force carbon flux towards malonyl-CoA. Metab Eng 13:578-587. http://dx.doi.org/10.1016/j.ymben.2011.06.008.
44. Chemler JA, Fowler ZL, McHugh KP, Koffas MA. 2010. Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering. Metab Eng 12:96-104. http://dx.doi.org/10 .1016/j.ymben.2009.07.003.
45. Xu H, Liu X, Yan Q, Yuan F, Gao Y. 2015. A novel copigment of quercetagetin for stabilization of grape skin anthocyanins. Food Chem 166:50-55. http://dx.doi.org/10.1016/j.foodchem.2014.05.125.
46. Wu J, Guan Y, Zhong Q. 2015. Yeast mannoproteins improve thermal stability of anthocyanins at pH 7.0. Food Chem 172:121-128. http://dx .doi.org/10.1016/j.foodchem.2014.09.059.