Identification of new ligands for the methionine biosynthesis transcriptional regulator (MetJ) by FAC-MS

Journal of Molecular Microbiology and Biotechnology

Volumn 22, Issue 4, 2012, Pages 205-214

  Mart Arbona, Ricardo ^a   Teshima, Munehiro ^a   Anderson, Penelope S ^a   Nowak Lovato, Kristy L ^a   Hong Geller, Elizabeth ^a   Unkefer, Clifford J ^a   Unkefer, Pat J ^a  

^a LOS ALAMOS NATIONAL LABORATORY   (United States)

Author keywords

5 Deoxy 5 (methylthio)adenosine; Adenine; Frontal affinity chromatography coupled to mass spectrometry; Methionine biosynthesis; Methionine salvage; MetJ; S adenosyl L methionine; Transcriptional regulator; Transcriptional regulator effector

Indexed keywords

5' METHYLTHIOADENOSINE; METHIONINE BIOSYNTHESIS TRANSCRIPTIONAL REGULATOR; METHIONINE DERIVATIVE; REGULATOR PROTEIN; S ADENOSYLMETHIONINE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; BINDING SITE; DISSOCIATION CONSTANT; ELUTION; FRONTAL AFFINITY CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; NONHUMAN; PROTEIN BINDING; TANDEM MASS SPECTROMETRY;

ADENINE; BACTERIAL PROTEINS; BINDING SITES; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DEOXYADENOSINES; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, BACTERIAL; GENETIC VECTORS; HIGH-THROUGHPUT SCREENING ASSAYS; LIGANDS; METHIONINE; PROTEIN BINDING; REPRESSOR PROTEINS; S-ADENOSYLMETHIONINE; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; THIONUCLEOSIDES; TIME FACTORS; TRANSCRIPTION, GENETIC;

EID: 84864816419     PISSN: 14641801     EISSN: 16602412     Source Type: Journal    
DOI: 10.1159/000339717     Document Type: Article

References (34)

1. Adomas A, Heller G, Olson A, Osborne J, Karlsson M, Nahalkova J, Van Zyl L, Sederoff R, Stenlid J, Finlay R, Asiegbu FO: Comparative analysis of transcript abundance in Pinus sylvestris after challenge with a saprotrophic, pathogenic or mutualistic fungus. Tree Physiol 2008; 28: 885-897. (Pubitemid 351967851)
2. Albers E: Metabolic characteristics and importance of the universal methionine salvage pathway recycling methionine from 5-methylthioadenosine. IUBMB Life 2009; 61: 1132-1142.
3. Annis DA, Nickbarg E, Yang X, Ziebell MR, Whitehurst CE: Affinity selection-mass spectrometry screening techniques for small molecule drug discovery. Curr Opin Chem Biol 2007; 11: 518-526. (Pubitemid 47588997)
4. Berger MF, Bulyk ML: Protein binding microarrays (PBMs) for rapid, high-throughput characterization of the sequence specificities of DNA binding proteins. Methods Mol Biol 2006; 338: 245-260.
5. Berger MF, Philippakis AA, Qureshi AM, He FS, Estep PW, 3rd, Bulyk ML: Compact, universal DNA microarrays to comprehensively determine transcription-factor binding site specificities. Nat Biotechnol 2006; 24: 1429-1435. (Pubitemid 44722107)
6. Calleri E, Temporini C, Caccialanza G, Massolini G: Target-based drug discovery: The emerging success of frontal affinity chromatography coupled to mass spectrometry. Chem Med Chem 2009; 4: 905-916.
7. Chan NW, Lewis DF, Rosner PJ, Kelly MA, Schriemer DC: Frontal affinity chromatography-mass spectrometry assay technology for multiple stages of drug discovery: Applications of a chromatographic biosensor. Anal Biochem 2003; 319: 1-12. (Pubitemid 36802137)
8. Cho BK, Barrett CL, Knight EM, Park YS, Palsson BO: Genome-scale reconstruction of the Lrp regulatory network in Escherichia coli . Proc Natl Acad Sci USA 2008a;105: 19462-19467.
9. Cho BK, Knight EM, Palsson BO: Genomewide identification of protein binding locations using chromatin immunoprecipitation coupled with microarray. Methods Mol Biol 2008b;439: 131-145.
10. Deng G, Sanyal G: Applications of mass spectrometry in early stages of target based drug discovery. J Pharm Biomed Anal 2006; 40: 528-538. (Pubitemid 43227375)
11. Fort S, Kim HS, Hindsgaul O: Screening for galectin-3 inhibitors from synthetic lacto-Nbiose libraries using microscale affinity chromatography coupled to mass spectrometry. J Org Chem 2006; 71: 7146-7154. (Pubitemid 44452892)
12. Geoghegan KF, Kelly MA: Biochemical applications of mass spectrometry in pharmaceutical drug discovery. Mass Spectrom Rev 2005; 24: 347-366. (Pubitemid 40665797)
13. Hermann JC, Marti-Arbona R, Fedorov AA, Fedorov E, Almo SC, Shoichet BK, Raushel FM: Structure-based activity prediction for an enzyme of unknown function. Nature 2007;448: 775-779. (Pubitemid 47266333)
14. Hughes JA: In vivo hydrolysis of S-adenosyl-L-methionine in Escherichia coli increases export of 5-methylthioribose. Can J Microbiol 2006; 52: 599-602. (Pubitemid 44258728)
15. LaMonte BL, Hughes JA: In vivo hydrolysis of s-adenosylmethionine induces the Met regulon of Escherichia coli . Microbiology 2006; 152: 1451-1459.
16. Merlin C, Gardiner G, Durand S, Masters M: The Escherichia coli MetD locus encodes an Abc transporter which includes Abc (MetN), YaeE (MetI), and YaeC (MetQ). J Bacteriol 2002; 184: 5513-5517. (Pubitemid 35024400)
17. Ng ES, Yang F, Kameyama A, Palcic MM, Hindsgaul O, Schriemer DC: High-throughput screening for enzyme inhibitors using frontal affinity chromatography with liquid chromatography and mass spectrometry. Anal Chem 2005; 77: 6125-6133. (Pubitemid 41436944)
18. Palcic MM, Zhang B, Qian X, Rempel B, Hindsgaul O: Evaluating carbohydrate-protein binding interactions using frontal affinity chromatography coupled to mass spectrometry. Methods Enzymol 2003; 362: 369-376. (Pubitemid 36993060)
19. Phillips SE, Manfield I, Parsons I, Davidson BE, Rafferty JB, Somers WS, Margarita D, Cohen GN, Saint-Girons I, Stockley PG: Cooperative tandem binding of Met repressor of Escherichia coli . Nature 1989; 341: 711-715. (Pubitemid 19260884)
20. Saint-Girons I, Duchange N, Cohen GN, Zakin MM: Structure and autoregulation of the metJ regulatory gene in Escherichia coli . J Biol Chem 1984; 259: 14282-14285. (Pubitemid 15224009)
21. Schena M, Shalon D, Davis RW, Brown PO: Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Science 1995; 270: 467-470.
22. Sekowska A, Kung HF, Danchin A: Sulfur metabolism in Escherichia coli and related bacteria: facts and fiction. J Mol Microbiol Biotechnol 2000; 2: 145-177. (Pubitemid 30218817)
23. Shoeman R, Redfield B, Coleman T, Greene RC, Smith AA, Brot N, Weissbach H: Regulation of methionine synthesis in Escherichia coli : effect of metJ gene product and s-adenosylmethionine on the expression of the metF gene. Proc Natl Acad Sci USA 1985; 82: 3601-3605. (Pubitemid 15243892)
24. Slon-Usakiewicz JJ, Dai JR, Ng W, Foster JE, Deretey E, Toledo-Sherman L, Redden PR, Pasternak A, Reid N: Global kinase screening. Applications of frontal affinity chromatography coupled to mass spectrometry in drug discovery. Anal Chem 2005a;77: 1268-1274. (Pubitemid 40327863)
25. Slon-Usakiewicz JJ, Ng W, Dai JR, Pasternak A, Redden PR: Frontal affinity chromatography with MS detection (FAC-MS) in drug discovery. Drug Discov Today 2005b;10: 409-416. (Pubitemid 40444656)
26. Smith AA, Greene RC, Kirby TW, Hindenach BR: Isolation and characterization of the product of the methionine-regulatory gene metJ of Escherichia coli K-12. Proc Natl Acad Sci USA 1985; 82: 6104-6108. (Pubitemid 16223907)
27. Temporini C, Ceruti S, Calleri E, Ferrario S, Moaddel R, Abbracchio MP, Massolini G: Development of an immobilized GPR17 receptor stationary phase for binding determination using frontal affinity chromatography coupled to mass spectrometry. Anal Biochem 2009; 384: 123-129.
28. Toledo-Sherman L, Deretey E, Slon-Usakiewicz JJ, Ng W, Dai JR, Foster JE, Redden PR, Uger MD, Liao LC, Pasternak A, Reid N: Frontal affinity chromatography with MS detection of EphB2 tyrosine kinase receptor. 2. Identification of small-molecule inhibitors via coupling with virtual screening. J Med Chem 2005; 48: 3221-3230. (Pubitemid 40628042)
29. VanGuilder HD, Vrana KE, Freeman WM: Twenty-five years of quantitative pcr for gene expression analysis. Biotechniques 2008; 44: 619-626. (Pubitemid 351656280)
30. Wang J, Zhang B, Fang J, Sujino K, Li H, Otter A, Hindsgaul O, Palcic MM, Wang PG: Frontal affinity chromatography coupled to mass spectrometry: an effective method for K d determination and screening of-gal derivatives binding to anti-gal antibodies (IgG). Carbohydrate Chem 2003; 22: 347-376. (Pubitemid 37412122)
31. Yi L, Li Z, Yuan K, Qu X, Chen J, Wang G, Zhang H, Luo H, Zhu L, Jiang P, Chen L, Shen Y, Luo M, Zuo G, Hu J, Duan D, Nie Y, Shi X, Wang W, Han Y, Li T, Liu Y, Ding M, Deng H, Xu X: Small molecules blocking the entry of severe acute respiratory syndrome coronavirus into host cells. J Virol 2004; 78: 11334-11339. (Pubitemid 39299674)
32. Zepperitz C, Hofner G, Wanner KT: Ms-binding assays: kinetic, saturation, and competitive experiments based on quantitation of bound marker as exemplified by the GABA transporter mgat1. Chem Med Chem 2006; 1: 208-217. (Pubitemid 43700301)
33. Zhang B, Palcic MM, Mo H, Goldstein IJ, Hindsgaul O: Rapid determination of the binding affinity and specificity of the mushroom polyporus squamous lectin using frontal affinity chromatography coupled to electrospray mass spectrometry. Glycobiology 2001a;11: 141-147. (Pubitemid 32331674)
34. Zhang B, Palcic MM, Schriemer DC, Alvarez-Manilla G, Pierce M, Hindsgaul O: Frontal affinity chromatography coupled to mass spectrometry for screening mixtures of enzyme inhibitors. Anal Biochem 2001b;299: 173-182. (Pubitemid 34053407)