A panorama of bacterial inulinases: Production, purification, characterization and industrial applications

International Journal of Biological Macromolecules

Volumn 96, Issue , 2017, Pages 312-322

  Singh, Ram Sarup ^a   Chauhan, Kanika ^a   Kennedy, John F ^b  

^a PUNJABI UNIVERSITY   (India)

^b Institute of Advanced Science and Technology   (United Kingdom)

Author keywords

Fructooligosaccharides; High fructose syrup; Inulinases; Purification; Solid state fermentation; Submerged fermentation

Indexed keywords

BACTERIAL ENZYME; FRUCTOSE; INULINASE; INULOOLIGOSACCHARIDE; OLIGOSACCHARIDE; UNCLASSIFIED DRUG; GLYCOSIDASE;

ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SUBSTRATE; ENZYME SYNTHESIS; INDUSTRY; NONHUMAN; PROTEIN EXPRESSION; REVIEW; SOLID STATE FERMENTATION; SUBMERGED FERMENTATION; SYRUP; BACTERIUM; BIOSYNTHESIS; BIOTECHNOLOGY; ENZYMOLOGY; FERMENTATION; ISOLATION AND PURIFICATION; METABOLISM; PROCEDURES;

BACTERIA; BIOTECHNOLOGY; FERMENTATION; GLYCOSIDE HYDROLASES; INDUSTRY;

EID: 85007237308     PISSN: 01418130     EISSN: 18790003     Source Type: Journal    
DOI: 10.1016/j.ijbiomac.2016.12.004     Document Type: Review

References (101)

1. [1] Ronkart, S.N., Blecker, C.S., Fougnies, C., Van Herck, J.C., Wouters, J., Paquot, M., Determination of physical changes of inulin related to sorption isotherms: an X-ray diffraction, modulated differential scanning calorimetry and environmental scanning electron microscopy study. Carbohydr. Polym. 63 (2006), 210–217.
2. [2] Fleming, S.E., GrootWassink, J.W.D., Murray, E.D., Production of high-fructose syrup from the tubers of the Jerusalem artichoke (Helianthus tuberosus L.). CRC Crit. Rev. Food Sci. Nutr. 12 (1979), 1–28.
3. [3] Ettalibi, M., Baratti, J.C., Purification properties and comparison of invertase, exoinulinases and endoinulinases of Aspergillus ficuum. Appl. Microbiol. Biotechnol. 26 (1987), 13–20.
4. [4] Verma, P.S., Prasad, M., The digestive physiology of Gryllodes sigillatus orthoptera gryllidae. Ind. J. Entomol. 37 (1975), 19–23.
5. [5] Mishra, S.C., Singh, P., Polysaccharide digestive enzymes in the larvae of Stromatium barbatum: a dry wood borer coleopteran cerambycidae. Mater. Organismen 13 (1987), 115–122.
6. [6] Edelman, J., Bacon, J.S.D., Transfructodation in extracts of the tubers of Helianthus tuberosus. Biochem. J. 49 (1951), 529–540.
7. [7] Singh, R.S., Enzymatic preparation of high fructose syrup from inulin. Panesar, P.S., Sharma, H.K., Sarkar, B.C., (eds.) Bioprocessing of Foods, 2011, Asiatech Publishing Inc, New Delhi, India, 77–98.
8. [8] Singh, R.S., Singh, R.P., Response surface optimization of endoinulinase production from a cost effective substrate by Bacillus safensis AS-08 for hydrolysis of inulin. Biocatal. Agric. Biotechnol. 3 (2014), 365–372.
9. [9] de Moura, F.A., Macagnan, F.T., da Silva, L.P., Oligosachharide production by hydrolysis of polysaccharides: a review. Int. J. Food Sci. Technol. 50 (2014), 275–281.
10. [10] Hu, N., Yuan, B., Sun, J., Wang, S.A., Li, F.L., Thermotolerant Kluyveromyces marxianus and Saccharomyces cerevisiae strains representing potentials for bioethanol production from Jerusalem artichoke by consolidated bioprocessing. Appl. Microbiol. Biotechnol. 95 (2012), 1359–1368.
11. [11] Chi, Z.-M., Zhang, T., Cao, T.-S.C., Liu, X.-Y., Cui, W., Zhao, C.-H., Biotechnological potential of inulin for bioprocesses. Bioresour. Technol. 102 (2011), 4295–4303.
12. [12] Liu, X.-Y., Chi, Z., Liu, G.-L., Wang, F., Madzak, C., Chi, Z.-M., Inulin hydrolysis and citric acid production from inulin using the surface-engineered Yarrowia lipolytica displaying inulinase. Metabol. Eng. 12 (2010), 469–476.
13. [13] Petrova, P., Velokova, P., Popova, L., Petrov, K., Direct conversion of chicory flour into (+) −lactic acid by the highly effective inulinase producer Lactobacillus paracasei DSM 23505. Bioresour. Technol. 186 (2015), 329–333.
14. [14] Li, A.X., Guo, L.-Z., Lu, W.-D., Alkaline inulinase production by a newly isolated bacterium Marinimicrobium sp. LS-A18 and inulin hydrolysis by the enzyme. World J. Microbiol. Biotechnol. 28 (2012), 81–89.
15. [15] Kato, K., Araki, T., Kitamura, T., Morita, N., Moori, M., Suzuki, Y., Purification and properties of a thermostable inulinase (β-D-Fructan Fructohydrolase) from Bacillus stearothermophilus KP1289. Starch/Stärke 51 (1999), 253–258.
16. [16] Gao, W., Bao, Y., Liu, Y., Zhang, X., Wang, J., An, L., Characterization of a thermo-stable endoinulinase from a new strain Bacillus smithii T7. Appl. Biochem. Biotechnol. 157 (2009), 498–506.
17. [17] Lu, W.-D., Li, A.-X., Guo, Q.-L., Production of novel alkalitolerant and thermostable inulinase from marine actinomycete Nocardiopsis sp. DN-K15 and inulin hydrolysis by the enzyme. Ann. Microbiol. 64 (2014), 441–449.
18. [18] Drent, W.J., Gottschal, J.C., Fermentation of inulin by a new strain of Clostridium thermoautotrophicum isolated from dahlia tubers. FEMS Microbiol. Lett. 78 (1991), 285–292.
19. [19] Cho, Y.J., Yun, J.W., Purification and characterization of an endoinulinase from Xanthomonas oryzae No. 5. Process Biochem. 37 (2002), 1325–1331.
20. [20] Uzunova, K., Vassileva, A., Ivanova, V., Spasova, D., Tonkova, A., Thermostable exo-inlinase production by semicontinuous cultivation of membrane-immobilized Bacillus sp. 11 cells. Process Biochem. 37 (2002), 863–868.
21. [21] Laowklom, N., Chantanaphan, R., Pinphanichakarn, P., Production, purification and characterization of inulinase from a newly isolated Streptomyces sp. CP01. Nat. Resour. 3 (2012), 137–144.
22. [22] Zhou, J., Gao, Y., Zhang, R., Mo, M., Tang, X., Li, J., Xu, B., Ding, J., Huang, Z., A novel low-temperature-active exo-inulinase identified based on molecular-activity strategy from Sphingobacterium sp. GN25 isolated from feces of Grus nigricollis. Process Biochem. 49 (2014), 1656–1663.
23. [23] Warchol, M., Perrin, S., Grill, J.-P., Schneider, F., Characterization of a purified β-fructofuranosidase from Bifidobacterium infantis ATCC 15697. Lett. Appl. Microbiol. 35 (2002), 462–467.
24. [24] Ehrmann, M.A., Korakli, M., Vogel, R.F., Identification of the gene for β-fructofuranosidase of Bifidobacterium lactis DSM10140T and characterization of the enzyme expressed in Escherichia coli. Curr. Microbiol. 46 (2003), 391–397.
25. [25] Janer, C., Rohr, L.M., Pelaez, C., Laloi, M., Cleusix, V., Requena, T., Meile, T., Hydrolysis of oligofructoses by the recombinant β-fructofuranosidase from Bifidobacterium lactis. Syst. Appl. Microbiol. 27 (2004), 279–285.
26. [26] Imamura, L., Hisamitsu, K., Kobashi, K., Purification and characterization of β fructofuranosidase from Bifidobacterium infantis. Biol. Pharm. Bull. 17 (1994), 596–602.
27. [27] Muramatsu, K., Onodera, S., Kikuchi, M., Shiomi, N., Purification and some properties of β-fructofuranosidase from Bifidobacterium adolescentis G1. Biosci. Biotechnol. Biochem. 57 (1993), 1681–1685.
28. [28] Omori, T., Ueno, K., Muramatsu, K., Kikuchi, M., Onodera, S., Shiomi, N., Characterization of recombinant β-fructofuranosidase from Bifidobacterium adolescentis G1. Chem. Cent. J., 4, 2010, 9.
29. [29] Ryan, S.M., Fitzgerald, G.F., van Sinderen, D., Transcriptional regulation and characterization of a novel β-fructofuranosidase-encoding gene from Bifidobacterium breve UCC2003. Appl. Environ. Microbiol. 71 (2005), 3475–3482.
30. [30] Tsujimoto, Y., Watanabe, A., Nakano, K., Watanabe, K., Matsui, H., Tsuji, K., Tsukihara, T., Suzuki, Y., Gene cloning expression, and crystallization of a thermostable exo-inulinase from Geobacillus stearothermophilus KP1289. Appl. Microbiol. Biotechnol. 62 (2003), 180–185.
31. [31] Zhou, J., Peng, M., Zhang, R.J., Li, J., Tang, X., Xu, B., Ding, J., Gao, Y., Ren, J., Huang, Z., Characterization of Sphingomonas sp. JB13 exo-inulinase: a novel detergent-, salt-, and protease-tolerant exo-inulinase. Extremophiles 19 (2015), 383–393.
32. [32] Franck, A., Technological functionality of inulin and oligofructose. Br. J. Nutr. 87 (2002), S287–S291.
33. [33] Pontes, A.G.O., Silva, K.L., da Cruz Fonseca, S.G., Soares, A.A., de Andrade Feitosa, J.P., Braz-Filho, R., Romero, N.R., Bandeira, M.A.M., Identification and determination of the inulin content in the roots of North east Brazilian species of Pombalia calceolaria L. Carbohydr. Polym. 149 (2016), 391–398.
34. [34] Mensink, M.A., Frijlink, H.W., van der Voort Maarschalk, K., Hinrichs, W.L.J., Inulin, a flexible oligosaccharide I: review of its physicochemical properties. Carbohydr. Polym. 130 (2015), 405–419.
35. [35] Singh, R.S., Chauhan, K., Production, purification, characterization and applications of fungal inulinases. Curr. Biotechnol, 5, 2016, 10.2174/2211550105666160512142330 (E-pub ahead of print).
36. [36] Singh, R.S., Singh, R.P., Yadav, M., Molecular and biochemical characterization of a new endoinulinase producing bacterial strain of Bacillus safensis AS-08. Biologia (Bratisl.) 68 (2013), 1028–1033.
37. [37] Gavrailov, S., Ivanova, V., Effects of nitrogen and carbon sources on the production of inulinase from strain Bacillus sp. SG113. ASN 3 (2016), 68–73.
38. [38] Müller, S.M., Seyfarth, W., Purification and substrate specificity of an extracellular fructanhydrolase from Lactobacillus paracasei ssp paracasei P 4134. New Phytol. 136 (1997), 89–96.
39. [39] Zherebtsov, N.A., Shelamova, S.A., Abramova, I.N., Biosynthesis of inulinases from Bacillus bacteria. Appl. Biochem. Microbiol. 38 (2002), 544–548.
40. [40] Naidoo, K., Ayyachamy, M., Permaul, K., Singh, S., Enhanced fructooligosaccharides and inulinase production by a Xanthomonas campestris pv. phaseoli KM 24 mutant. Bioprocess Biosyst. Eng. 32 (2009), 689–695.
41. [41] Mazutti, M.A., Skrowonski, A., Boni, G., Zabot, G.L., Silva, M.F., de Oliveira, D., Di Luccio, M., Filho, F.M., Rodrigues, M.I., Treichel, H., Partial characterization and inulinases obtained by submerged and solid-state fermentation using agroindustrial residues as substrates: a comparative study. Appl. Biochem. Biotechnol. 160 (2010), 682–693.
42. [42] Angel, S.J., Kavitha, C., Vidyadharani, G., Roy, P., Dhandapani, R., Isolation of inulinase producing bacteria from sugarcane soil. Int. J. Appl. Biol. Pharm. Technol. 3 (2012), 320–326.
43. [43] Belamri, M., Sassi, A.H., Savart, M., Tantaoui-Elaraki, A., Cottin, P., Purification and properties of an extracellular inulinase like β-fructosidase from Bacillus stearothermophilus. Lett. Appl. Microbiol. 19 (1994), 410–413.
44. [44] Brierley, M.R., Steel, R., Agitation-aeration in submerged fermentation II. Effect of two solid disperse phase on oxygen absorption in a fermentor. Appl. Microbiol. 7 (1959), 57–61.
45. [45] Pandey, A., Solid-state fermentation. Biochem. Eng. J. 13 (2003), 81–84.
46. [46] Perez-Guerra, N., Torrado-Agrasar, A., Lopez-Macias, C., Pastrana, L., Main characteristics and applications of solid substrate fermentation. Electron. J. Environ. Agric. Food Chem. 2 (2003), 343–350.
47. [47] Couto, S.R., Sanromán, M.Á., Application of solid-state fermentation to food industry—a review. J. Food Eng. 76 (2006), 291–302.
48. [48] Selvakumar, P., Pandey, A., Solid state fermentation for the synthesis of inulinase from Staphylococcus sp. and Kluyveromyces marxianus. Process Biochem. 34 (1999), 851–855.
49. [49] Ayyachamy, M., Khelawan, K., Pillay, D., Permaul, K., Singh, S., Production of inulinase by Xanthomonas campestris pv phaseoli using onion (Allium cepa) and garlic (Allium sativum) peels in solid state cultivation. Lett. Appl. Microbiol. 45 (2007), 439–444.
50. [50] Dilipkumar, M., Rajasimman, M., Rajamohan, N., Application of statistical design for the production of inulinase by Streptomyces sp. using pressmud. Front. Chem. Sci. Eng. 5 (2011), 463–470.
51. [51] Gao, J., Xu, Y.-Y., Yang, H.-M., Xu, H., Xue, F., Li, S., Feng, X.-H., Gene cloning, expression and characterization of an exoinulinase from Paenibacillus polymyxa ZJ-9. Appl. Biochem. Biotechnol. 173 (2014), 1419–1430.
52. [52] Kwon, Young-Man, Kim, H.-Y., Choi, Y.-J., Cloning and characterization of Pseudomonas mucidolens exoinulinase. J. Microbiol. Biotechnol. 10 (2000), 238–243.
53. [53] Kuzuwa, S., Yokoi, K.J., Kondo, M., Kimoto, H., Yamakawa, A., Taketo, A., Kodaira, K.-I., Properties of the inulinase gene levH1 of Lactobacillus casei IAM 1045; cloning, mutational and biochemical characterization. Gene 495 (2012), 154–162.
54. [54] Menéndez, C., Martínez, D., Trujillo, L.E., Mazola, Y., González, E., Pérez, E.R., Hernández, L., Constitutive high-level expression of a codon-optimized β-fructosidase gene from the hyperthermophile Thermotoga maritima in Pichia pastoris. Appl. Microbiol. Biotechnol. 97 (2013), 1201–1212.
55. [55] Elyachioui, M., Hornez, J.P., Tallliez, R., General properties of extracellular bacterial inulinase. J. Appl. Bacteriol. 73 (1992), 514–519.
56. [56] Haraguchi, K., Hayashi, K., Kasumi, T., Purification and properties of inulinase from Arthrobacter globiformis S64-1. Starch/Stärke 1 (1990), 28–30.
57. [57] Liebl, W., Brem, D., Gotschilch, A., Analysis for the gene for β-fructosidase (invertase, inulinase) of the thermophilic bacterium Thermotoga maritima, and characterisation of the enzyme expressed in Escherichia coli. Appl. Microbiol. Biotechnol. 50 (1998), 55–64.
58. [58] Paludan-Müller, C., Gram, L., Rattray, F.P., Purification and characterisation of an extracellular fructan β-fructosidase from a Lactobacillus pentosus strain isolated from fermented fish, system. Appl. Microbiol. 25 (2002), 3–20.
59. [59] Jedrzejczak-Krzepkowska, M., Tkaczuk, K.L., Bielecki, S., Biosynthesis, purification and characterization of β-fructofuranosidase from Bifidobacterium longum KN29.1. Process Biochem. 46 (2011), 1963–1972.
60. [60] Picard, C., Fioramonti, J., Francois, A., Robinson, T., Neant, F., Matuchansky, C., Review article: bifidobacteria as probiotic agents − physiological effects and clinical benefits. Aliment. Pharmacol. Ther. 22 (2005), 495–512.
61. [61] Bujacz, A., Jedrzejczak-Krzepkowska, M., Bielecki, S., Redzynia, I., Bujacz, G., Crystal structures of the apo form of β-fructofuranosidase from Bifidobacterium longum and its complex with fructose. FEBS J. 278 (2011), 1728–1744.
62. [62] Efstathiou, I., Reysset, G., Truffaut, N., A study of inulinase activity in the Clostridium acetobutylicum strain ABKn8. Appl. Microbiol. Biotechnol. 25 (1986), 143–149.
63. [63] Kwon, H.-J., Jeon, S.-J., You, D.-J., Kim, K.-H., Jeong, Y.-K., Kim, Y.-H., Kim, Y.-M., Kim, B.-W., Cloning and characterization of an exoinulinase from Bacillus polymyxa. Biotechnol. Lett. 25 (2003), 155–159.
64. [64] Tohamy, E.Y., Purification and characterization of exoinulinase enzyme from Streptomyces grisenus. Pak. J. Biol. Sci. 9 (2006), 911–916.
65. [65] Liu, G.-L., Chi, Z., Chi, Z.-M., Molecular characterization and expression of microbial inulinase genes. Crit. Rev. Microbiol. 39 (2013), 152–165.
66. [66] Ohta, K., Akimoto, H., Matsuda, S., Toshimitsu, D., Nakamura, T., Molecular cloning and sequence analysis of two endoinulinase genes from Aspergillus niger. Biosci. Biotechnol. Biochem. 62 (1998), 1731–1738.
67. [67] Uhm, T.B., Chae, K.-S., Lee, D.W., Kim, H.-S., Cassart, J.-P., Vandenhaute, J., Cloning and nucleotide sequence of the endoinulinase-encoding gene, inu2, from Aspergillus ficuum. Biotechnol. Lett. 20 (1998), 809–812.
68. [68] Onodera, S., Murakami, T., Ito, H., Mori, H., Matsui, H., Honma, M., Chiba, S., Shiomi, N., Molecular cloning and nucleotide sequences of cDNA and gene encoding endoinulinase from Penicillium purpurogenum. Biosci. Biotech. Biochem. 60 (1996), 1780–1785.
69. [69] Laloux, O., Cassart, J.-P., Delcour, J., Van Beeumen, J., Vandenhaute, J., Cloning and sequencing of the inulinase gene from Kluyveromyces marxianus var. marxianus ATCC 12424. FEBS 289 (1991), 64–68.
70. [70] Martin, I., Debarbouille, M., Ferrari, E., Klier, A., Rapoport, G., Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase. Mol. Gen. Genet. 208 (1987), 177–184.
71. [71] Taussig, R., Carlson, M., Nucleotide sequence of the yeast SUC2 gene for invertase. Nuc. Acids Res. 11 (1983), 1943–1954.
72. [72] -Il. Kang, S., -Il. Kim, S., Molecular cloning and sequence analysis of an endo-inulinase gene from Arthrobacter sp. Biotechnol. Lett. 21 (1999), 569–574.
73. [73] Azhar, M., Natalia, D., Syukur, S., Jamsari, Vovien, Gene fragments that encodes inulin hydrolysis enzyme from genomic Bacillus licheniformis: isolation by PCR technique using new primers. Int. J. Biol. Chem. 9 (2015), 59–69.
74. [74] Bin, L., Jingyun, W., Yongming, B., Fan, Z., Lijia, A., Characterization of a key amino acid residues in active sites of inulinase from Bacillus smithii T7 by chemical modification. Chin. J. Catal. 30 (2009), 673–678.
75. [75] Zherebtsov, N.A., Abramova, I.N., Shelamova, S.A., Popova, T.N., Identification of catalytically active groups in inulinase from Bacillus polymyxa 722. Appl. Biochem. Micrbiol. 39 (2003), 544–548.
76. [76] Singh, P.K., Joseph, J., Goyal, S., Grover, A., Shukla, P., Functional analysis of the binding model of microbial inulinases using docking and molecular dynamics simulation. J. Mol. Model., 22, 2016, 69.
77. [77] Wolfgang, W., Südzucker, A.G.M., Fructose. Hubert, P., (eds.) Electronic Ullmann's Encyclopedia of Industrial Chemistry, 2004, Wiley-VCH GmbH & Co. KGaA, Germany, 10.1002/14356007.a12_047.pub2.
78. [78] Ylikahari, R.H., Kahonen, M.T., Hassinen, I., Modification of metabolic effects of ethanol by fructose. Acta Med. Scand. Suppl. 542 (1972), 141–150.
79. [79] Pawan, G.L.S., Fructose. Birch, G.G., Green, L.F., (eds.) Molecular Structure and Function of Food Carbohydrates, 1973, Applied Science Publishers, London, 65–80.
80. [80] Reddy, K.M., Deepthi, S.S., Jayachandra, S.Y., Parameshwar, A.B., Agsar, D., Bikshapathi, E., Sulochana, M.B., In silico structural analysis for exoinulinases in proteomes of Streptomyces sp. using PDB structures as templates. Int. J. Curr. Microbiol. App. Sci. 4 (2015), 858–867.
81. [81] Ganaie, M.A., Lateef, A., Gupta, U.S., Enzymatic trends of fructooligosaccharides production by microorganisms. Appl. Biochem. Biotechnol. 172 (2014), 2143–2159.
82. [82] Park, J.P., Bae, J.T., You, D.J., Kim, B.W., Yun, J.W., Production of inulooligosaccharides from inulin by a novel endoinulinase from Xanthomonas sp. Biotechnol. Lett. 21 (1999), 1043–1046.
83. [83] Yokota, A., Yamauchi, O., Tomita, F., Production of inulotriose from inulin by inulin-degrading enzyme from Streptomyces rochei E87. Lett. Appl. Microbiol. 21 (1995), 330–333.
84. [84] Yun, J.W., Choi, Y.J., Song, C.H., Song, S.K., Microbial production of inulo-oligosaccharides by an endoinulinase from Pseudomonas sp. expressed in Escherichia coli. J. Biosci. Bioeng. 87 (1999), 291–295.
85. [85] Kim, C.H., Rhee, S.K., Ethanol production from Jerusalem artichoke by inulinase and Zymomonas mobilis. Appl. Biochem. Biotechnol. 23 (1990), 171–180.
86. [86] Gao, J., Xu, H., Li, Q.-j., Feng, X.-h., Li, S., Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3-butanediol. Bioresour. Technol. 101 (2010), 7076–7082.
87. [87] Ge, X.-Y., Qian, H., Zhang, W.-G., Improvement of L-lactic acid production from Jerusalem artichoke tubers by mixed culture of Aspergillus niger and Lactobacillus sp. Bioresour. Technol. 100 (2009), 1872–1874.
88. [88] Kim, D.-M., Kim, H.-S., Continuous production of gluconic acid and sorbitol from Jerusalem artichoke and glucose using an oxidoreductase of Zymomonas mobilis and inulinase. Biotechnol. Bioeng. 39 (1992), 336–342.
89. [89] Saha, B.C., Production of mannitol from inulin by simultaneous enzymatic saccharification and fermentation with Lactobacillus intermedius NRRL B-3693. Enzyme Microb. Technol. 39 (2006), 991–995.
90. [90] Li, S.Z., Chan-Halbrendt, C., Ethanol production in (the) People's Republic of China: potential and technologies. Appl. Energy 86 (2009), S162–S169.
91. [91] Szambelan, K., Nowak, J., Czarnecki, Z., Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers. Biotechnol. Lett. 26 (2004), 845–848.
92. [92] Allais, J.-J., Torres, E.F., Baratti, J., Continuous production of ethanol with Zymomonas mobilis growing on Jerusalem artichoke juice. Biotechnol. Bioeng. 29 (1987), 778–782.
93. [93] Sun, L.-H., Wang, X.-D., Dai, J.-Y., Xiu, Z.-L., Microbial production of 2,3-butanediol from Jerusalem artichoke tubers by Klebsiella pneumoniae. Appl. Microbiol. Biotechnol. 82 (2009), 847–852.
94. [94] Kang, Su-ll, Chang, Y.-J., Oh, S.-J., Su-ll Kim, Purification and properties of an endoinulinase from an Arthrobacter sp. Biotechnol. Lett. 20 (1998), 983–986.
95. [95] Haraguchi, K., Akita, K., Hayashi, K., Kubo, N., Production of β-D-fructofuranosidase by Arthrobacter globiformis S64-1. Rep. Natl. Food Res. Inst. 51 (1987), 1–5.
96. [96] Vullo, D.L., Cotto, C.E., Sineriz, F., Characteristics of an inulinase produced by Bacillus subtilis 430A, a strain isolated from the rhizosphere of Vernonia herbacea (Vell Rusby). Appl. Environ. Microbiol. 57 (1991), 2392–2394.
97. [97] Looten, Ph., Blanchet, D., Vandecasteele, J.P., The β-fructofuranosidase activities of a strain of Clostridium acetobutylicum grown on inulin. Appl. Microbiol. Biotechnol. 25 (1987), 419–425.
98. [98] Allais, J.-J., Kammoun, S., Blanc, P., Girard, C., Baratti, J.C., Isolation and characterization of bacterial strains with inulinase activity. Appl. Environ. Microbiol. 52 (1986), 1086–1090.
99. [99] Müller, M., Steller, J., Comparative studies of the degradation of grass fructan and inulin by strains of Lactobacillus paracasei subsp. paracasei and Lactobacillus plantarum. J. Appl. Bacteriol. 78 (1995), 229–236.
100. [100] Selvakumar, P., Pandey, A., Comparative study on inulinase synthesis by Staphylococcus sp. and Kluyveromyces marxainus under submerged fermentation. Bioresour. Technol. 69 (1999), 123–127.
101. [101] Sharma, A.D., Kainth, S., Gill, P.K., Inulinase production using garlic (Allium sativum) powder as a potential substrate in Streptomyces sp. J. Food Eng. 77 (2006), 486–491.