Gene cloning, expression, and characterization of an exo-inulinase from Paenibacillus polymyxa ZJ-9

Applied Biochemistry and Biotechnology

Volumn 173, Issue 6, 2014, Pages 1419-1430

  Gao, Jian ^a   Xu, You Yong ^a   Yang, Hong Mei ^c   Xu, Hong ^b   Xue, Feng ^a   Li, Sha ^b   Feng, Xiao Hai ^b  

^a YANCHENG INSTITUTE OF TECHNOLOGY   (China)

^b NANJING TECH UNIVERSITY   (China)

^c Yancheng Biology Engineering Higher School   (China)

Author keywords

Characterization; Cloning; Exo inulinase; Paenibacillus polymyxa ZJ 9; R,R 2,3 butanediol

Indexed keywords

CHARACTERIZATION; CLONING; ELECTROPHORESIS; ENZYME ACTIVITY; ESCHERICHIA COLI; FERMENTATION; GEL PERMEATION CHROMATOGRAPHY; GENE ENCODING; HYDROLYSIS; INDUSTRIAL APPLICATIONS; PROTEINS; PURIFICATION; SODIUM DODECYL SULFATE;

ESCHERICHIA COLI BL21; EXO-INULINASE; GEL-FILTRATION CHROMATOGRAPHY; JERUSALEM ARTICHOKE TUBERS; PAENIBACILLUS POLYMYXA; R,R-2,3-BUTANEDIOL; SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS; SUBSTRATE SPECIFICITY;

POLYSACCHARIDES;

INULIN; INULINASE; RECOMBINANT ENZYME; SUCROSE; 2,3-BUTYLENE GLYCOL; BACTERIAL PROTEIN; BUTANEDIOL; GLYCOSIDASE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; ENZYME ACTIVITY; ENZYME SPECIFICITY; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; GENE EXPRESSION; HYDROLYSIS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PAENIBACILLUS POLYMYXA; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SEQUENCE ANALYSIS; AMINO ACID SEQUENCE; BIOTECHNOLOGY; CHEMISTRY; ENZYMOLOGY; FERMENTATION; GENETICS; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MICROBIOLOGY; MOLECULAR GENETICS; PAENIBACILLUS; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HELIANTHUS TUBEROSUS; PAENIBACILLUS POLYMYXA;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BIOTECHNOLOGY; BUTYLENE GLYCOLS; CLONING, MOLECULAR; FERMENTATION; GENES, BACTERIAL; GLYCOSIDE HYDROLASES; INULIN; KINETICS; MOLECULAR SEQUENCE DATA; PAENIBACILLUS; RECOMBINANT PROTEINS; SEQUENCE HOMOLOGY, AMINO ACID; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY;

EID: 84904765264     PISSN: 02732289     EISSN: 15590291     Source Type: Journal    
DOI: 10.1007/s12010-014-0950-y     Document Type: Article

References (29)

1. Wang, J. M., Zhang, T., Chi, Z., Liu, G. L., & Chi, Z. M. (2011). 18S rDNA integration of the exo-inulinase gene into chromosomes of the high ethanol producing yeast Saccharomyces sp. W0 for direct conversion of inulin to bioethanol. Biomass and Bioenergy, 35, 3032-3039.
2. Li, S. Z., & Chan-Halbrendt, C. (2009). Ethanol production in (the) People's Republic of China: potential and technologies. Applied Energy, 86, S162-169.
3. Kwon, H. J., Jeon, S. J., You, D. J., Kim, K.H., Jeong, Y. K., Kim, Y.H., Kim, Y.M., & Kim, B.W. (2003). Cloning and characterization of an exoinulinase from Bacillus polymyxa. Biotechnology Letters, 25, 155-159.
4. Vandamme, E. J., & Derycke, D. G. (1983). Microbial inulinases: fermentation process, properties and applications. Advances in Applied Microbiology, 29, 139-176.
5. Neagu, C., & Bahrim, G. (2011). Inulinases-a versatile tool for biotechnology. Innovative Romanian Food Biotechnology, 9, 1-11.
6. Chi, Z. M., Zhang, T., Cao, T. S., Liu, X. Y., Cui, W., & Zhao, C. H. (2011). Biotechnological potential of inulin for bioprocesses. Bioresource Technology, 102, 4295-4303.
7. Goosen, C., & Dijkhuizen, L. (2008). Exo-inulinase of Aspergillus niger N402: a hydrolytic enzyme with significant transfructosylating activity. Biocatalysis and Biotransformation, 26, 49-58.
8. Kango, N., & Jain, S. C. (2011). Production and properties of microbial inulinases: recent advances. Food Biotechnology, 25, 165-212.
9. Singh, P., & Gill, P. K. (2006). Production of inulinases: recent advances. Food Technology Biotechnology, 44(2), 151-162. (Pubitemid 44033839)
10. Sun, L. H., Wang, X. D., Dai, J. Y., & Xiu, Z. L. (2009). Microbial production of 2,3-butanediol from Jerusalem artichoke tubers by Klebsiella pneumoniae. Applied Microbiology and Biotechnology, 82, 847- 852.
11. Celiñska, E., & Grajek, W. (2009). Biotechnological production of 2,3-butanediol - current state and prospects. Biotechnology Advances, 27, 715-725.
12. Gao, J., Xu, H., Li, Q. J., Feng, X. H., & Li, S. (2010). Optimization of medium for one-step fermentation of inulin extract from Jerusalem artichoke tubers using Paenibacillus polymyxa ZJ-9 to produce R,R-2,3- butanediol. Bioresource Technology, 101, 7076-7082.
13. Ui, S., Masuda, H., & Muraki, H. (1984). Laboratory-scale production of acetoin isomers (D(-) and L(+)) by bacterial fermentation. Journal Fermentation Technology, 62, 151-156.
14. Fergus, G. P., Michael, G., & Carole, T. (1988). A numerical classification of the genus Bacillus. Journal of General Microbiology, 134, 1847-1882.
15. Holt, J. G., Sneath, P. H. A., Mair, N. S., & Sharpe, M. E. (1986). 9th ed., Bergey's manual of systematic bacteriology, vol. 2, 9th ed (pp. 1104-1139). Baltimore: Williams & Wilkins.
16. Zhou, X. L., Shen, W., Rao, Z. M., Wang, Z. X., & Zhuge, J. (2004). A rapid method for preparation of fungal chromosome DNA. Weishengwu Xue Tongbao, 31, 89-92.
17. Xu, Z., Qing, Y. J., Li, S., Feng, X. H., Xu, H., & Ouyang, P. K. (2011). A novel L-arabinose isomerase from Lactobacillus fermentum CGMCC2921 for D-tagatose production: gene cloning, purification and characterization. Journal of Molecular Catalysis B: Enzymatic, 70, 1-7.
18. Xiao, R., Tanida, M., & Takao, S. (1988). Inulinase from Chrysosporium pannorum. Journal Fermentation Technology, 66, 553-558.
19. Nguyen, Q. D., Mattes, F., Hoschke, A., Rezessy-Szabo, J., & Bhat, M. K. (1999). Production, purification and identification of fructooligosaccharides produced by β-fructofuranosidase from Aspergillus niger IMI 303386. Biotechnology Letters, 21, 183-186. (Pubitemid 29171431)
20. Szambelan, K., Nowak, J., & Czarnecki, Z. (2004). Use of Zymomonas mobilis and Saccharomyces cerevisiae mixed with Kluyveromyces fragilis for improved ethanol production from Jerusalem artichoke tubers. Biotechnology Letters, 26, 845-848. (Pubitemid 38982866)
21. Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426-428.
22. T. Applied Microbiology and Biotechnology, 96, 1517-1526.
23. Liebl, W., Feil, R., Gabelsberger, J., Kellermann, J., & Schleifer, K. H. (1992). Purification and characterization of a novel thermostable 4-α-glucanotransferase of Thermotoga maritima cloned in Es-cherichia coli. European Journal of Biochemistry, 207, 81-88.
24. Reddy, V. A., & Maley, F. (1990). Identification of an active-site residue in yeast invertase by affinity labeling and site-directed mutagenesis. The Journal of Biological Chemistry, 265, 10817-10820. (Pubitemid 20200065)
25. Nagem, R. A., Rojas, A. L., Golubev, A. M., Korneeva, O. S., Enevskava, E. V., Kulminskava, A. A., Neustroev, K. N., & Polikarpov, I. (2004). Crystal structure of exo-inulinase from Aspergillus awamori: The enzyme fold and structural determinants of substrate recognition. Journal of Molecular Biology, 344, 471-480.
26. Martin, I., Debarbouille, M., Ferrari, E., Klier, A., & Rapoport, G. (1987). Characterization of the levanase gene of Bacillus subtilis which shows homology to yeast invertase. Molecular Genetics and Genomics, 208, 177-184.
27. Gunasekaran, P., Karunakaran, T., Cami, B., Mukundan, A. G., Preziosi, L., & Baratti, J. (1990). Cloning and sequencing of the sacA gene: characterization of a sucrase from Zymomonas mobilis. Journal of Bacteriology, 172, 6727-6735.
28. Pandey, A., Soccol, C. R., & Fontana, J. D. (1999). Recent developments in microbial inulinases. Applied Biochemistry and Biotechnology, 81, 35-52.
29. Rouwenhorst, R. J., & Visser, L. E. (1988). Production, distribution and kinetic properties of inulinase in continuous cultures of Kluyveromyces marxianus CBS6556. Applied and Environmental Microbiology, 54, 1311-1137.