Combinatorial pathway optimization in Escherichia coli by directed co-evolution of rate-limiting enzymes and modular pathway engineering

Biotechnology and Bioengineering

Volumn 113, Issue 12, 2016, Pages 2661-2669

  Lv, Xiaomei ^a   Gu, Jiali ^b   Wang, Fan ^a   Xie, Wenping ^a   Liu, Min ^a   Ye, Lidan ^a   Yu, Hongwei ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b HUZHOU UNIVERSITY   (China)

Author keywords

directed co evolution; isoprene; MEP pathway; modular pathway engineering

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING; INDUSTRIAL RESEARCH; ISOPRENE; METABOLIC ENGINEERING; METABOLISM; PROTEINS; RECOMBINANT PROTEINS; THROUGHPUT;

CO-EVOLUTION; COMBINATORIAL STRATEGIES; HIGH THROUGHPUT SCREENING METHODS; MEP PATHWAYS; METABOLIC INTERMEDIATES; MICROBIAL BIOSYNTHESIS; PATHWAY ENGINEERINGS; RATE-LIMITING ENZYMES;

BIOCHEMICAL ENGINEERING;

1 DEOXYXYLULOSE 5 PHOSPHATE REDUCTASE; 1 DEOXYXYLULOSE 5 PHOSPHATE SYNTHASE; ISOMERASE; ISOPENTENYLDIPHOSPHATE DIMETHYLALLYLDIPHOSPHATE ISOMERASE; ISOPRENE; LYCOPENE; OXIDOREDUCTASE; PHOSPHATE; SYNTHETASE; UNCLASSIFIED DRUG; 1,3 BUTADIENE DERIVATIVE; BACTERIAL PROTEIN; HEMITERPENE; PENTANE; RECOMBINANT PROTEIN;

ARTICLE; CARBOHYDRATE METABOLISM; COEVOLUTION; COMBINATORIAL LIBRARY; CONTROLLED STUDY; ESCHERICHIA COLI; HIGH THROUGHPUT SCREENING; METABOLIC ENGINEERING; METHYLERYTHRITOL PHOSPHATE PATHWAY; MOLECULAR DYNAMICS; NONHUMAN; PROCESS OPTIMIZATION; PROMOTER REGION; PROTEIN ENGINEERING; WILD TYPE; BIOSYNTHESIS; COMBINATORIAL CHEMISTRY; DIRECTED MOLECULAR EVOLUTION; GENETIC ENHANCEMENT; GENETICS; METABOLIC CLEARANCE RATE; METABOLISM; PHYSIOLOGY; PROCEDURES;

BACTERIAL PROTEINS; BIOSYNTHETIC PATHWAYS; BUTADIENES; COMBINATORIAL CHEMISTRY TECHNIQUES; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETIC ENHANCEMENT; HEMITERPENES; METABOLIC CLEARANCE RATE; METABOLIC ENGINEERING; PENTANES; RECOMBINANT PROTEINS;

EID: 84992451563     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.26034     Document Type: Article

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