Enhanced activity of Rhizomucor miehei lipase by directed evolution with simultaneous evolution of the propeptide

Applied Microbiology and Biotechnology

Volumn 96, Issue 2, 2012, Pages 443-450

  Wang, Jue ^a   Wang, Dan ^a   Wang, Bo ^a   Mei, Zhuo Hang ^a   Liu, Ji ^a   Yu, Hong Wei ^a  

^a ZHEJIANG UNIVERSITY   (China)

Author keywords

Activity; Directed evolution; Propeptide; Protein folding; RML

Indexed keywords

CATALYTIC DOMAINS; CATALYTIC RATES; DIRECTED EVOLUTION; E. COLI; FULL-LENGTH PROTEINS; PROPEPTIDES; RHIZOMUCOR MIEHEI LIPASE; RML; SEQUENCE ANALYSIS; SHORT SEQUENCES; SYNERGISTIC EFFECT;

ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING; ISOMERS; PROTEIN FOLDING; RATE CONSTANTS; THERMODYNAMIC PROPERTIES;

PROTEINS;

PROPEPTIDE; PROTEIN PRECURSOR; TRIACYLGLYCEROL LIPASE; UNCLASSIFIED DRUG;

CATALYSIS; COLIFORM BACTERIUM; ENZYME ACTIVITY; MUTATION; PEPTIDE; PROTEIN;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ESCHERICHIA COLI; MOLECULAR CLONING; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; RHIZOMUCOR; RHIZOMUCOR MIEHEI; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

BIOCATALYSIS; CATALYTIC DOMAIN; DIRECTED MOLECULAR EVOLUTION; FUNGAL PROTEINS; KINETICS; LIPASE; MUTAGENESIS, SITE-DIRECTED; RHIZOMUCOR;

ESCHERICHIA COLI; RHIZOMUCOR MIEHEI;

EID: 84866508244     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4049-5     Document Type: Article

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