Enhanced enantioselectivity of a carboxyl esterase from Rhodobacter sphaeroides by directed evolution

Applied Microbiology and Biotechnology

Volumn 97, Issue 11, 2013, Pages 4897-4906

  Ma, Jingbo ^a   Wu, Lian ^b   Guo, Fei ^a   Gu, Jiali ^a   Tang, Xiaoling ^a   Jiang, Ling ^a   Liu, Ji ^a   Zhou, Jiahai ^b   Yu, Hongwei ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b SHANGHAI INSTITUTE OF ORGANIC CHEMISTRY   (China)

Author keywords

Directed evolution; Enantioselective esterase; Model substrate; Molecular dynamics simulation; Substrate range

Indexed keywords

DIRECTED EVOLUTION; ENANTIOSELECTIVE; MODEL SUBSTRATES; MOLECULAR DYNAMICS SIMULATIONS; SUBSTRATE RANGE;

ENANTIOSELECTIVITY; ENZYMES; ESTERS; MOLECULAR DYNAMICS;

SUBSTRATES;

CARBOXYLESTERASE; ESTER; MANDELIC ACID DERIVATIVE;

BACTERIUM; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVITY; MOLECULAR ANALYSIS; MUTATION; SUBSTRATE;

ARTICLE; CRYSTAL STRUCTURE; CRYSTALLIZATION; ENANTIOMER; ENANTIOSELECTIVITY; ENZYME CONFORMATION; HIGH THROUGHPUT SCREENING; HYDROGEN BOND; HYDROLYSIS; MOLECULAR DYNAMICS; MUTATION; NONHUMAN; PROTEIN ENGINEERING; PROTEIN EXPRESSION; RHODOBACTER SPHAEROIDES;

AMINO ACID SUBSTITUTION; BINDING SITES; CARBOXYLIC ESTER HYDROLASES; CRYSTALLOGRAPHY, X-RAY; DIRECTED MOLECULAR EVOLUTION; MANDELIC ACIDS; MODELS, MOLECULAR; MOLECULAR DYNAMICS SIMULATION; MUTATION, MISSENSE; PROTEIN BINDING; PROTEIN CONFORMATION; RHODOBACTER SPHAEROIDES; SUBSTRATE SPECIFICITY;

RHODOBACTER SPHAEROIDES;

EID: 84878012848     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4396-2     Document Type: Article

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