High-level production of lycopene in metabolically engineered E. coli

Process Biochemistry

Volumn 44, Issue 8, 2009, Pages 899-905

  Kim, Sung Woo ^a,b   Kim, Jae Bum ^a   Ryu, Ji Myung ^a   Jung, Joon Ki ^a   Kim, Jung Hoe ^b  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Inocula dependence; Lycopene; Pantoea ananatis; Plasmid copy number; Synthetic operon; Temperature shift

Indexed keywords

INOCULA DEPENDENCE; LYCOPENE; PANTOEA ANANATIS; PLASMID COPY NUMBER; SYNTHETIC OPERON;

ESCHERICHIA COLI;

ESCHERICHIA COLI; PANTOEA; PANTOEA ANANATIS;

EID: 66049098477     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2009.04.018     Document Type: Article

References (42)

1. Umeno D., and Arnold F.H. A C35 carotenoid biosynthetic pathway. Appl Environ Microbiol 69 (2003) 3573-3579
2. Di Mascio P., Kaiser S., and Sies H. Lycopene as the most efficient biological carotenoid singlet oxygen quencher. Arch Biochem Biophys 274 (1989) 532-538
3. Armstrong G.A. Genetics of eubacterial carotenoid biosynthesis: a colorful tale. Annu Rev Microbiol 51 (1997) 629-659
4. Clinton S.K. Lycopene: chemistry, biology, and implications for human health and disease. Nutr Rev 56 (1998) 35-51
5. Misawa N., Nakagawa M., Kobayashi K., Yamano S., Izawa Y., Nakamura K., et al. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J Bacteriol 172 (1990) 6704-6712
6. Misawa N., Yamano S., and Ikenaga H. Production of beta-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora. Appl Environ Microbiol 57 (1991) 1847-1849
7. Miura Y., Kondo K., Saito T., Shimada H., Fraser P.D., and Misawa N. Production of the carotenoids lycopene, β-carotene, and astaxanthin in the food yeast Candida utilis. Appl Environ Microbiol 64 (1998) 1226-1229
8. Lee P.C., and Schmidt-Dannert C. Metabolic engineering towards biotechnological production of carotenoids in microorganisms. Appl Microbiol Biotechnol 60 (2002) 1-11
9. Yoon S.H., Kim J.E., Lee S.H., Park H.M., Choi M.S., Kim J.Y., et al. Engineering the lycopene synthetic pathway in E. coli by comparison of the carotenoid genes of Pantoea agglomerans and Pantoea ananatis. Appl Microbiol Biotechnol 74 (2007) 131-139
10. Kim S.W., Jung W.H., Ryu J.M., Kim J.B., Jang H.W., Jo Y.B., et al. Identification of an alternative translation initiation site for the Pantoea ananatis lycopene cyclase (crtY) gene in E. coli and its evolutionary conservation. Protein Expr Purif 58 (2008) 23-31
11. Kim S.W., Kim J.B., Jung W.H., Kim J.H., and Jung J.K. Overproduction of β-carotene from metabolically engineered Escherichia coli. Biotechnol Lett 28 (2006) 897-904
12. Yoon S.H., Park H.M., Kim J.E., Lee S.H., Choi M.S., Kim J.Y., et al. Increased beta-carotene production in recombinant Escherichia coli harboring an engineered isoprenoid precursor pathway with mevalonate addition. Biotechnol Prog 23 (2007) 599-605
13. Ruther A., Misawa N., Böger P., and Sandmann G. Production of zeaxanthin in Escherichia coli transformed with different carotenogenic plasmids. Appl Microbiol Biotechnol 48 (1997) 162-167
14. Tao L., Wilczek J., Odom J.M., and Cheng Q. Engineering a beta-carotene ketolase for astaxanthin production. Metab Eng 8 (2006) 523-531
15. Farmer W.R., and Liao J.C. Improving lycopene production in Escherichia coli by engineering metabolic control. Nat Biotechnol 18 (2000) 533-537
16. Wang C.W., Oh M.K., and Liao J.C. Directed evolution of metabolically engineered Escherichia coli for carotenoid production. Biotechnol Prog 16 (2000) 922-926
17. Farmer W.R., and Liao J.C. Precursor balancing for metabolic engineering of lycopene production in Escherichia coli. Biotechnol Prog 17 (2001) 57-61
18. Kajiwara S., Fraser P.D., Kondo K., and Misawa N. Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli. Biochem J 324 (1997) 421-426
19. Alper H., Miyaoku K., and Stephanopoulos G. Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nat Biotechnol 23 (2005) 612-616
20. Alper H., Jin Y.S., Moxley J.F., and Stephanopoulos G. Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli. Metab Eng 7 (2005) 155-164
21. Yuan L.Z., Rouvière P.E., LaRossa R.A., and Suh W. Chromosomal promoter replacement of the isoprenoid pathway for enhancing carotenoid production in E. coli. Metab Eng 8 (2006) 79-90
22. Yoon S.H., Lee Y.M., Kim J.E., Lee S.H., Lee J.H., Kim J.Y., et al. Enhanced lycopene production in Escherichia coli engineered to synthesize isopentenyl diphosphate and dimethylallyl diphosphate from mevalonate. Biotechnol Bioeng 94 (2006) 1025-1032
23. Zahiri H.S., Yoon S.H., Keasling J.D., Lee S.H., Kim S.W., Yoon S.C., et al. Coenzyme Q10 production in recombinant Escherichia coli strains engineered with heterologous decaprenyl diphosphate synthase gene and foreign mevalonate pathway. Metab Eng 8 (2006) 406-416
24. Das A., Yoon S.H., Lee S.H., Kim J.Y., Oh D.K., and Kim S.W. An update on microbial carotenoid production: application of recent metabolic engineering tools. Appl Microbiol Biotechnol 77 (2007) 505-512
25. Birnbaum S., and Bailey J.E. Plasmid presence changes the relative levels of many host cell proteins and ribosome components in recombinant Escherichia coli. Biotechnol Bioeng 37 (1991) 736-745
26. Bentley W.E., Mirjalili N., Anderson D.C., Davis R.H., and Kompala D.S. Plasmid-encoded protein: the principal factor in the metabolic burden associated with recombinant bacteria. Biotechnol Bioeng 35 (1990) 668-681
27. Vind J., Sorensen M., Rasmussen M., and Pedeson S. Synthesis of proteins in E. coli is limited by the concentration of free ribosomes. J Mol Biol 231 (1993) 678-688
28. Glick B.R. Metabolic load and heterologous gene expression. Biotechnol Adv 13 (1995) 247-261
29. Bollag D.M., Rozycki M.D., and Edelstein S.J. Protein methods. 2nd ed. (1996), Wiley-Liss, Inc., New York pp. 34-35
30. Lee S.Y., Mermelstein L.D., and Papoutsakis E.T. Determination of plasmid copy number and stability in Clostridium acetobutylicum ATCC 824. FEMS Microbiol Lett 108 (1993) 319-324
31. Umeno D., Tobias A.V., and Arnold F.H. Evolution of the C30 carotenoid synthase crtM for function in a C40 pathway. J Bacteriol 184 (2002) 6690-6699
32. O'Connor M., Peifer M., and Bender W. Construction of large DNA segments in Escherichia coli. Science 244 (1989) 1307-1312
33. Jones S.A., and Melling J. Persistence of pBR322-related plasmids in Escherichia coli grown in chemostat cultures. FEMS Microbiol Lett 22 (1984) 239-243
34. Warnes A., and Stephenson J.R. The insertion of large pieces of foreign genetic material reduces the stability of bacterial plasmids. Plasmid 16 (1986) 116-123
35. Smith M.A., and Bidochka M.J. Bacterial fitness and plasmid: the importance of culture conditions and plasmid size. Can J Microbiol 44 (1998) 351-355
36. Alper H., Miyaoku K., and Stephanopoulos G. Characterization of lycopene-overproducing E. coli strains in high cell density fermentations. Appl Microbiol Biotechnol 72 (2006) 968-974
37. Vadali R.V., Fu Y., Bennett G.N., and San K.Y. Enhanced lycopene productivity by manipulation of carbon flow to isopentenyl diphosphate in Escherichia coli. Biotechnol Prog 21 (2005) 1558-1561
38. BAD promoter. J Bacteriol 177 (1995) 4121-4130
39. Lee P.C., Mijts B.N., and Schmidt-Dannert C. Investigation of factors influencing production of the monocyclic carotenoid toluene in metabolically engineered Escherichia coli. Appl Microbiol Biotechnol 65 (2004) 538-546
40. Sandmann G., Albrecht M., Schurr G., Knörzer O., and Böger P. The biotechnological potential and design of novel carotenoids by gene combination in Escherichia coli. Trends Biotechnol 17 (1999) 233-237
41. Martin V.J., Yoshikuni Y., and Keasling J.D. The in vivo synthesis of plant sesquiterpenes by Escherichia coli. Biotechnol Bioeng 75 (2001) 497-503
42. Tao L., Jackson R.E., Rouviere P.E., and Cheng Q. Isolation of chromosomal mutations that affect carotenoid production in Escherichia coli: mutations alter copy number of ColE1-type plasmids. FEMS Microbiol Lett 243 (2005) 227-233