Conventional gene targeting protocols lead to loss of targeted cells when applied to a silent gene locus in primary fibroblasts

Journal of Biotechnology

Volumn 125, Issue 2, 2006, Pages 185-193

  Marques, Margarita M ^a   Thomson, Alison J ^b   McCreath, Kenneth J ^c   McWhir, Jim ^b  

^a UNIVERSITY OF LEÓN   (Spain)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

^c UNIVERSITY OF VALENCIA   (Spain)

Author keywords

Beta casein; COL1A1; Gene targeting; Promoter trap; Sheep primary fibroblasts; Transcriptionally silent genes

Indexed keywords

CELL CULTURE; CELLS; GENES;

BETA-CASEIN; COL1A1; GENE TARGETING; PROMOTER TRAP; SHEEP PRIMARY FIBROBLASTS; TRANSCRIPTIONALLY SILENT GENES;

BIOTECHNOLOGY;

BETA CASEIN; PROCOLLAGEN;

ANIMAL CELL; ARTICLE; CELL GROWTH; CELL ISOLATION; FETUS; FIBROBLAST; GENE EXPRESSION; GENE LOCUS; GENE SILENCING; GENE TARGETING; LIVESTOCK; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SHEEP; SOUTHERN BLOTTING;

ANIMALS; BLOTTING, SOUTHERN; CELLS, CULTURED; COLLAGEN TYPE I; FIBROBLASTS; GENE TARGETING; GENETIC VECTORS; MODELS, GENETIC; POLYMERASE CHAIN REACTION; PROMOTER REGIONS (GENETICS); SHEEP;

OVIS;

EID: 33746900838     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.03.009     Document Type: Article

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