Rapid Protein Depletion in Human Cells by Auxin-Inducible Degron Tagging with Short Homology Donors

Cell Reports

Volumn 15, Issue 1, 2016, Pages 210-218

  Natsume, Toyoaki ^a   Kiyomitsu, Tomomi ^b,c   Saga, Yumiko ^d,e,f   Kanemaki, Masato T ^a,c,e  

^a NATIONAL INSTITUTE OF GENETICS   (Japan)

^b NAGOYA UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d NATIONAL INSTITUTE OF GENETICS   (Japan)

^e GRADUATE UNIVERSITY FOR ADVANCED STUDIES   (Japan)

^f UNIVERSITY OF TOKYO   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AUXIN; COHESIN; CYTOPLASM PROTEIN; DYNEIN ADENOSINE TRIPHOSPHATASE; NUCLEAR PROTEIN; ARABIDOPSIS PROTEIN; CELL SURFACE RECEPTOR; F BOX PROTEIN; INDOLEACETIC ACID DERIVATIVE; TIR1 PROTEIN, ARABIDOPSIS;

ALLELE; ANIMAL CELL; ARTICLE; AUXIN INDUCIBLE DEGRON TAGGING; CELL CLONING; CRISPR CAS SYSTEM; CULTURE MEDIUM; EMBRYO; GENETIC TRANSFECTION; HCT116 CELL LINE; HUMAN; HUMAN CELL; MOUSE; MOUSE EMBRYONIC STEM CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEPLETION; PROTEIN SUBUNIT; SEQUENCE HOMOLOGY; ANIMAL; DRUG EFFECTS; EMBRYONIC STEM CELL; ESSENTIAL GENE; GENE TARGETING; GENETICS; HCT 116 CELL LINE; METABOLISM; PROCEDURES; PROTEIN DEGRADATION;

ANIMALS; ARABIDOPSIS PROTEINS; CRISPR-CAS SYSTEMS; EMBRYONIC STEM CELLS; F-BOX PROTEINS; GENE TARGETING; GENES, ESSENTIAL; HCT116 CELLS; HUMANS; INDOLEACETIC ACIDS; MICE; PROTEOLYSIS; RECEPTORS, CELL SURFACE; SEQUENCE HOMOLOGY;

EID: 84963606981     PISSN: None     EISSN: 22111247     Source Type: Journal    
DOI: 10.1016/j.celrep.2016.03.001     Document Type: Article

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