Engineering a genetically encoded competitive inhibitor of the KEAP1-NRF2 interaction via structure-based design and phage display

Protein Engineering, Design and Selection

Volumn 29, Issue 1, 2015, Pages 1-9

  Guntas, Gurkan ^a   Lewis, Steven M ^a   Mulvaney, Kathleen M ^a,b   Cloer, Erica W ^a,b   Tripathy, Ashutosh ^a   Lane, Thomas R ^a   Major, Michael B ^a,b   Kuhlman, Brian ^a,b  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

Author keywords

KEAP1; NRF2; protein design; redox regulation; Rosetta molecular modeling program

Indexed keywords

AMINO ACIDS; BINS; PROTEINS;

KEAP1; NRF2; PROTEIN DESIGN; REDOX REGULATION; ROSETTA;

TRANSCRIPTION;

BOVINE SERUM ALBUMIN; HEME OXYGENASE 1; KELCH LIKE ECH ASSOCIATED PROTEIN 1; MERCAPTOETHANOL; OLIGONUCLEOTIDE; SCAFFOLD PROTEIN; SIGNAL PEPTIDE; TRANSCRIPTION FACTOR NRF2; ANTIBODY; KEAP1 PROTEIN, HUMAN; NFE2L2 PROTEIN, HUMAN; RECOMBINANT PROTEIN;

ANION EXCHANGE; ARTICLE; CIRCULAR DICHROISM; COMPETITIVE INHIBITION; CRYSTAL STRUCTURE; GEL FILTRATION CHROMATOGRAPHY; GENETIC CODE; GENETIC ENGINEERING; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; ISOTHERMAL TITRATION CALORIMETRY; MOLECULAR MODEL; MUTAGENESIS; PHAGE DISPLAY; PRIORITY JOURNAL; PROTEIN DATABASE; PROTEIN EXPRESSION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SURFACE PLASMON RESONANCE; WESTERN BLOTTING; ANTAGONISTS AND INHIBITORS; CELL SURFACE DISPLAY; CHEMISTRY; GENETICS; HEK293 CELL LINE; METABOLISM; OXIDATION REDUCTION REACTION; PROCEDURES; PROTEIN ENGINEERING;

ANTIBODIES; CELL SURFACE DISPLAY TECHNIQUES; HEK293 CELLS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; KELCH-LIKE ECH-ASSOCIATED PROTEIN 1; MODELS, MOLECULAR; NF-E2-RELATED FACTOR 2; OXIDATION-REDUCTION; PROTEIN ENGINEERING; RECOMBINANT PROTEINS;

EID: 84959915528     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzv055     Document Type: Article

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