Development and optimization of a binding assay for the XIAP BIR3 domain using fluorescence polarization

Analytical Biochemistry

Volumn 332, Issue 2, 2004, Pages 261-273

  Nikolovska Coleska, Zaneta ^a   Wang, Renxiao ^a   Fang, Xueliang ^a   Pan, Hongguang ^b   Tomita, York ^b   Li, Peng ^c   Roller, Peter P ^c   Krajewski, Krzysztof ^c   Saito, Naoyuki G ^d   Stuckey, Jeanne A ^d   Wang, Shaomeng ^a  

^a UNIVERSITY OF MICHIGAN COMPREHENSIVE CANCER CENTER   (United States)

^b GEORGETOWN UNIVERSITY   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

^d UNIVERSITY OF MICHIGAN   (United States)

Author keywords

Apoptosis; Cheng Prusoff equation; Fluorescence polarization assay; High throughput screening; Ki calculation; Mathematical model; Smac DIABLO; XIAP

Indexed keywords

BINDING ENERGY; CELL DEATH; MATHEMATICAL MODELS; MOLECULES; PEPTIDES; POLARIZATION; PROBES;

CHENG-PRUSOFF EQUATION; FLUORESCENCE POLARIZATION; HIGH THROUGHPUT SCREENING; SMAC/DIABLO; XIAP;

FLUORESCENCE;

ACCURACY; AMINO ACID SEQUENCE; APOPTOSIS; ARTICLE; BINDING AFFINITY; BINDING ASSAY; CALCULATION; CANCER CELL; COMPETITIVE INHIBITION; COMPUTER PROGRAM; DYNAMICS; FLUORESCENCE; MATHEMATICAL ANALYSIS; MEASUREMENT; MOLECULE; PH; POLAROGRAPHY; PRIORITY JOURNAL; PROTEIN DOMAIN; QUANTITATIVE ANALYSIS; SCREENING;

EID: 7444262377     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.05.055     Document Type: Article

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