Cloning-independent expression and analysis of ω-transaminases by use of a cell-free protein synthesis system

Applied and Environmental Microbiology

Volumn 76, Issue 18, 2010, Pages 6295-6298

  Kwon, Yong Chan ^a   Lee, Kyung Ho ^a   Kim, Ho Cheol ^a   Han, Kyuboem ^b   Seo, Joo Hyun ^c   Kim, Byung Gee ^c   Kim, Dong Myung ^a  

^a Chungnam National University   (South Korea)

^b Hanson Biotech Co Ltd   (South Korea)

^c Seoul National University   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL COLONIES; CELL FREE PROTEIN SYNTHESIS; CELL-FREE EXPRESSION; ENZYMATIC ACTIVITIES; INFORMATION GAP; MICROBIAL ENZYMES; NUCLEOTIDE SEQUENCES; PROTEIN FUNCTIONS; SUBSTRATE SPECIFICITY; UNIVERSAL PLATFORM;

CATALYSTS; CLONE CELLS; CLONING; ENZYMES;

GENE EXPRESSION;

AMINOTRANSFERASE; PRIMER DNA;

BACTERIUM; COLONY; ENZYME ACTIVITY; GENE EXPRESSION; GENETIC ANALYSIS; POLYMERASE CHAIN REACTION; PROTEIN; SUBSTRATE;

ARTICLE; CELL FREE SYSTEM; COLORIMETRY; ENZYME SPECIFICITY; GENE EXPRESSION; GENETICS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; POLYMERASE CHAIN REACTION;

CELL-FREE SYSTEM; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; COLORIMETRY; DNA PRIMERS; GENE EXPRESSION; HIGH-THROUGHPUT SCREENING ASSAYS; NUCLEIC ACID AMPLIFICATION TECHNIQUES; POLYMERASE CHAIN REACTION; SUBSTRATE SPECIFICITY; TRANSAMINASES;

BACTERIA (MICROORGANISMS);

EID: 77956813514     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00029-10     Document Type: Article

References (23)

1. Christen, P., and D. E. Metzler. 1985. Transaminases. Wiley, New York, NY.
2. Crowley, L. V. 1967. The Reitman-Frankel colorimetric transaminase procedure in suspected myocardial, infarction. Clin. Chem. 13:482-487.
3. Entzeroth, M. 2003. Emerging trends in high-throughput screening. Curr. Opin. Pharmacol. 3:522-529.
4. Goddard, J. P., and J. L. Reymond. 2004. Recent advances in enzyme assays. Trends Biotechnol. 22:363-370.
5. Gorbalenya, A. E., E. V. Koonin, and M. M. C. Lai. 1991. Putative papainrelated thiol proteases of positive-strand RNA viruses-identification of rubivirus and aphthovirus proteases and delineation of a novel conserved domain associated with proteases of rubivirus, alpha- and coronaviruses. FEBS Lett. 288:201-205.
6. Hertzberg, R. P., and A. J. Pope. 2000. High-throughput screening: new technology for the 21st century. Curr. Opin. Chem. Biol. 4:445-451.
7. Jewett, M. C., K. A. Calhoun, A. Voloshin, J. J. Wuu, and J. R. Swartz. 2008. An integrated cell-free metabolic platform for protein production and synthetic biology. Mol. Syst. Biol. 4:10.
8. Kang, S. H., D. M. Kim, H. J. Kim, S. Y. Jun, and K. Y. Lee. 2005. Cell-free production of aggregation-prone proteins in soluble and active forms. Biotechnol. Prog. 21:1412-1419.
9. Katzen, F., G. Chang, and W. Kudlicki. 2005. The past, present and future of cell-free protein synthesis. Trends Biotechnol. 23:150-156.
10. Kim, D. M., T. Kigawa, C. Y. Choi, and S. Yokoyama. 1996. A highly efficient cell-free protein synthesis system from Escherichia coli. Eur. J. Biochem. 239:881-886.
11. Kim, T. W., J. W. Keum, I. S. Oh, C. Y. Choi, C. G. Park, and D. M. Kim. 2006. Simple procedures for the construction of a robust and cost-effective cell-free protein synthesis system. J. Biotechnol. 126:554-561.
12. Mardis, E. R. 2008. The impact of next-generation sequencing technology on genetics. Trends Genet. 24:133-141.
13. Murthy, T. V., W. Wu, Q. Q. Qiu, Z. Shi, J. LaBaer, and L. Brizuela. 2004. Bacterial cell-free system for high-throughput protein expression and a comparative analysis of Escherichia coli cell-free and whole cell expression systems. Protein Expr. Purif. 36:217-225.
14. Pereira, D. A., and J. A. Williams. 2007. Origin and evolution of high throughput screening. Br. J. Pharmacol. 152:53-61.
15. Reitman, S., and S. Frankel. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28:56-63.
16. Sawasaki, T., T. Ogasawara, R. Morishita, and Y. Endo. 2002. A cell-free protein synthesis system for high-throughput proteomics Proc. Natl. Acad. Sci. U. S. A. 99:14652-14657.
17. Schagger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfatepolyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
18. Schmidt, A. J., D. A. Ryjenkov, and M. Gomelsky. 2005. The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains. J. Bacteriol. 187:4774-4781.
19. Shimizu, Y., Y. Kuruma, B. W. Ying, S. Umekage, and T. Ueda. 2006. Cell-free translation systems for protein engineering. FEBS J. 273:4133-4140.
20. Shin, J. S., H. Yun, J. W. Jang, I. Park, and B. G. Kim. 2003. Purification, characterization, and molecular cloning of a novel amine:pyruvate transaminase from Vibrio fluvialis JS17. Appl. Microbiol. Biotechnol. 61:463-471.
21. Spirin, A. S. 2004. High-throughput cell-free systems for synthesis of functionally active proteins. Trends Biotechnol. 22:538-545.
22. Turner, N. J. 2003. Directed evolution of enzymes for applied biocatalysis. Trends Biotechnol. 21:474-478.
23. Wahler, D., and J. L. Reymond. 2001. High-throughput screening for biocatalysts. Curr. Opin. Biotechnol. 12:535-544.