Evaluating fermentation effects on cell growth and crude extract metabolic activity for improved yeast cell-free protein synthesis

Biochemical Engineering Journal

Volumn 91, Issue , 2014, Pages 140-148

  Choudhury, Alaksh ^a,b   Hodgman, C Eric ^a,b   Anderson, Mark J ^a,b   Jewett, Michael C ^a,b,c  

^a NORTHWESTERN UNIVERSITY   (United States)

^b Northwestern University   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Cell free protein synthesis; Defined media; Enzyme biocatalysis; Fermentation; Growth kinetics; Modelling yeast

Indexed keywords

AMINO ACIDS; FERMENTATION; GROWTH KINETICS; METABOLISM; PHYSIOLOGY; YEAST;

CELL FREE PROTEIN SYNTHESIS; CHEMICALLY DEFINED MEDIAS; DEFINED MEDIA; ENZYME BIOCATALYSIS; FERMENTATION CONDITIONS; METABOLIC ACTIVITY; PRECURSOR MONOMER; PROTEIN SYNTHESIS; CRUDE EXTRACT; YEAST CELL;

BIOSYNTHESIS; FERMENTATION;

CELL EXTRACT; AMINO ACID; LUCIFERASE; MONOMER; NUCLEOTIDE; PROTEIN PRECURSOR;

ALCOHOL PRODUCTION; AMINO ACID METABOLISM; ARTICLE; BATCH FERMENTATION; CARBON BALANCE; CELL DENSITY; CELL FREE PROTEIN SYNTHESIS; CELL FREE SYSTEM; CELL GROWTH; CELL METABOLISM; CELL VIABILITY; FUNGAL BIOMASS; GLUCOSE INTAKE; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; SYNTHETIC BIOLOGY; YEAST CELL; CHEMICAL COMPOSITION; CONTROLLED STUDY; CRUDE EXTRACT; CULTURE MEDIUM; CULTURE OPTIMIZATION; CULTURE TECHNIQUE; EXTRACT; FERMENTATION; IN VITRO STUDY; METABOLIC ACTIVITY ASSAY; METABOLIC REGULATION; REPRODUCIBILITY; WILD TYPE;

EID: 84907335243     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2014.07.014     Document Type: Article

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