Molecular determinants of peculiar properties of a Pleurotus ostreatus laccase: Analysis by site-directed mutagenesis

Enzyme and Microbial Technology

Volumn 45, Issue 6-7, 2009, Pages 507-513

  Autore, Flavia ^a   Del Vecchio, Claudia ^b   Fraternali, Franca ^a   Giardina, Paola ^b   Sannia, Giovanni ^b   Faraco, Vincenza ^b  

^a KING'S COLLEGE LONDON   (United Kingdom)

^b UNIVERSITY OF NAPLES FEDERICO II   (Italy)

Author keywords

C terminal tail; Enzyme stability; Phenol oxidases; Rational mutagenesis

Indexed keywords

ALKALINE PH; C-TERMINAL EXTENSIONS; C-TERMINAL TAIL; CATALYTIC PROPERTIES; CONFORMATIONAL CHANGE; CONSERVED REGIONS; ENZYME STABILITY; ENZYME STRUCTURES; HIGH STABILITY; LACCASES; LOCAL ENVIRONMENTS; MOLECULAR DETERMINANTS; PLEUROTUS OSTREATUS; RATIONAL MUTAGENESIS; SITE DIRECTED MUTAGENESIS; STABILITY PROPERTIES; SUBSTRATE AFFINITY;

AMINES; AMINO ACIDS; BINDING ENERGY; BINDING SITES; ENZYMES; MUTAGENESIS; ORGANIC ACIDS; PHENOLS;

STABILITY;

ARGININE; ASPARTIC ACID; LACCASE; MUTANT PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; ENZYME ANALYSIS; ENZYME BINDING; ENZYME CONFORMATION; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBSTRATE; GENE MUTATION; GENE REARRANGEMENT; GENETIC CONSERVATION; MOLECULAR DYNAMICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PLEUROTUS OSTREATUS; SITE DIRECTED MUTAGENESIS;

BASIDIOMYCOTA; PLEUROTUS OSTREATUS;

EID: 70349785028     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2009.08.004     Document Type: Article

References (45)

1. Thurston F. The structure and function of fungal laccases. Microbiology 140 (1994) 19-26
2. Ullah M.A., Bedford C.T., and Evans C.S. Reactions of pentachlorophenol with laccase from Coriolus versicolor. Appl Microbiol Biotechnol 53 (2000) 230-234
3. Bourbonnais R., and Paice M.G. Oxidation of non-phenolic substrates-an expanded role for laccase in lignin biodegradation. FEBS Lett 267 (1990) 99-102
4. Mayer A.M., and Staples R.C. Laccase: new functions for an old enzyme. Phytochemistry 60 (2002) 551-565
5. Solomon E.I., Sundaram U.M., and Machonkin T.E. Multicopper oxidases and oxygenases. Chem Rev 96 (1996) 2563-2605
6. Ferraroni M., Myasoedova N.M., Schmatchenko V., Leontievsky A.A., Golovleva L.A., Scozzafava A., et al. Crystal structure of a blue laccase from Lentinus tigrinus: evidences for intermediates in the molecular oxygen reductive splitting by multicopper oxidases. BMC Struct Biol 7 (2007) 60 10.1186/1472-6807-7-60
7. Ducros V., Brzozowski A.M., Wilson K.S., Brown S.H., Østergaard P., Schneider P., et al. Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 Å resolution. Nat Struct Biol 5 (1998) 310-316
8. Piontek K., Antorini M., and Choinowski T. Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-Å resolution containing a full complement of coppers. J Biol Chem 277 (2002) 37663-37669
9. Hakulinen N., Kiiskinen L.L., Kruus K., Saloheimo M., Paananen A., Koivula A., et al. Crystal structure of a laccase from Melanocarpus albomyces with an intact trinuclear copper site. Nat Struct Biol 9 (2002) 601-605
10. Garavaglia S., Cambria M.T., Miglio M., Ragusa S., Iacobazzi V., Palmieri F., et al. The structure of Rigidoporus lignosus laccase containing a full complement of copper ions, reveals an asymmetrical arrangement for the T3 copper pair. J Mol Biol 342 (2004) 1519-1531
11. Matera I., Gullotto A., Tilli S., Ferraroni M., Scozzafava A., and Briganti F. Crystal structure of the blue multicopper oxidase from the white-rot fungus Trametes trogii complexed with p-toluate. Inorg Chim Acta 361 14-15 (2008) 4129-4137
12. Bertrand T., Jolivalt C., Briozzo P., Caminade E., Joly N., Madzak C., et al. Crystal structure of a four-copper laccase complexed with an arylamine: insights into substrate recognition and correlation with kinetics. Biochemistry 41 (2002) 7325-7333
13. Enguita F.J., Marcal D., Martins L.O., Grenha R., Henriques A.O., Lindley P.F., et al. Substrate and dioxygen binding to the endospore coat laccase from Bacillus subtilis. J Biol Chem 279 (2004) 23472-23476
14. Pezzella C., Autore F., Giardina P., Piscitelli A., Sannia G., and Faraco V. The Pleurotus ostreatus laccase multi-gene family: isolation and heterologous expression of new family members. Curr Genet 55 1 (2008) 45-57
15. Palmieri G., Cennamo G., and Sannia G. Remazol brilliant blue R decolourisation by the fungus Pleurotus ostreatus and its oxidative enzymatic system. Enz Microb Technol 36 (2005) 17-24
16. Palmieri G., Giardina P., and Sannia G. Laccase-mediated Remazol brilliant blue R decolourization in a fixed-bed bioreactor. Biotechnol Prog 21 (2005) 1436-1441
17. Faraco V., Pezzella C., Miele A., Giardina P., and Sannia G. Bio-remediation of colored industrial wastewaters by the white-rot fungi Phanerochaete chrysosporium and Pleurotus ostreatus and their enzymes. Biodegradation 20 2 (2009) 209-220
18. Faraco V., Pezzella C., Giardina P., Piscitelli A., Vanhulle S., and Sannia G. Decolourization of textile dyes by the white-rot fungi Phanerochaete chrysosporium and Pleurotus ostreatus. J Chem Technol Biotechnol 84 (2009) 414-419
19. Giardina P., Palmieri G., Scaloni A., Fontanella B., Faraco V., Cennamo G., et al. Protein and gene structure of a blue laccase from Pleurotus ostreatus. Biochem J 341 (1999) 655-663
20. Piscitelli A., Giardina P., Mazzoni C., and Sannia G. Recombinant expression of Pleurotus ostreatus laccases in Kluyveromyces lactis and Saccharomyces cerevisiae. Appl Microbiol Biotechnol 69 (2005) 428-439
21. Schwede T., Kopp J., Guex N., and Peitsch M.C. SWISS-MODEL: an automated protein homology-modeling server. Nucleic Acids Res 31 (2003) 3381-3385
22. Festa G., Autore F., Fraternali F., Giardina P., and Sannia G. Development of new laccases by directed evolution: functional and computational analyses. Proteins 72 (2007) 25-34
23. Berendsen H.J.C., van der Spoel D., and van Drunen R. GROMACS: a message-passing parallel molecular dynamics implementation. Comput Phys Commun 91 (1995) 43-56
24. Daura X., Mark A.E., and van Gunsteren W.F. Parametrization of aliphatic CHn united atoms of GROMOS96 force field. J Comput Chem 19 (1998) 535-547
25. Berendsen H.J.C., Postma J.P.M., van Gunsteren W.F., Hermans J., and Pullman B. Interaction models for water in relation to protein hydration. Intermol Forces (1981) 331-342
26. Berendsen H.J.C., Postma J.P.M., van Gunsteren W.F., Dinola A., and Haak J.R. Molecular dynamics with coupling to an external bath. J Chem Phys 81 (1984) 3684-3690
27. Hess B., Bekker H., Berendsen H.J.C., and Fraaije J. LINCS: a linear constraint solver for molecular simulations. J Comput Chem 18 (1997) 1463-1472
28. Darden T., Perera L., Li L., and Pedersen L. New tricks for modelers from the crystallography toolkit: the particle mesh Ewald algorithm and its use in nucleic acid simulations. Struct Fold Des 7 (1999)
29. Humphrey W., Dalke A., and Schulten K. VMD-visual molecular dynamics. J Mol Graph 14 (1996) 33-38
30. Simossis V.A., and Heringa J. PRALINE: a multiple sequence alignment toolbox that integrates homology-extended and secondary structure information. Nucleic Acids Res 33 (2005) 289-294
31. Fernandez Larrea J., and Stahl U. Isolation and characterization of a laccase gene from Podospora anserina. Mol Gen Genet 252 (1996) 539-551
32. Germann U.A., Muller G., Hunziker P.E., and Lerch K. Characterization of 2 allelic forms of Neurospora crassa laccase amino-terminal and carboxyl-terminal processing of a precursor. J Biol Chem 263 (1988) 885-896
33. Kiiskinen L.L., and Saloheimo M. Molecular cloning and expression in Saccharomyces cerevisiae of a laccase gene from the ascomycete Melanocarpus albomyces. Appl Environ Microb 70 (2004) 137-144
34. Bulter T., Alcalde M., Sieber V., Meinhold P., Schlachtbauer C., and Arnold F.H. Functional expression of a fungal laccase in Saccharomyces cerevisiae by directed evolution. Appl Environ Microb 69 (2003) 987-995
35. Zumárraga M., Camarero S., Shleev S., Martínez-Arias A., Ballesteros A., Plou F.J., et al. Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra. Proteins 71 1 (2008) 250-260
36. Zumarraga M., Vaz Domínguez C., Camarero S., Shleev S., Polaina J., Martínez-Arias A., et al. Combinatorial Saturation Mutagenesis of the Myceliophthora thermophila Laccase T2 mutant: the connection between the C-terminal plug and the conserved 509vsg511tripeptide. Comb Chem High Throughput Screen 11 (2008) 807-816
37. Gelo-Pujic M., Kim H.H., Butlin N.G., and Palmore G.T. Electrochemical studies of a truncated laccase produced in Pichia pastoris. Appl Environ Microbiol 65 (1999) 5515-5521
38. Giardina P., Autore F., Faraco V., Festa G., Palmieri G., Piscitelli A., et al. Structural characterization of heterodimeric laccases from Pleurotus ostreatus. Appl Microbiol Biotechnol 75 (2007) 1293-1300
39. Faraco V., Ercole C., Festa G., Giardina P., Piscitelli A., and Sannia G. Heterologous expression of heterodimeric laccases from Pleurotus ostreatus in Kluyveromyces lactis. Appl Microbiol Biotechnol 77 (2008) 1329-1335
40. Madzak C., Mimmi M.C., Caminade E., Brault A., Baumberger S., Briozzo P., et al. Shifting the optimal pH of activity for a laccase from the fungus Trametes versicolor by structure-based mutagenesis. Protein Eng Des Sel 19 (2006) 77-84
41. Xu F. Effects of redox potential and hydroxide inhibition on the pH activity profile of fungal laccases. J Biol Chem 272 (1997) 924-928
42. Ceylan H., Kubilay S., Aktas N., and Sahiner N. An approach for prediction of optimum reaction conditions for laccase-catalyzed bio-transformation of 1-naphthol by response surface methodology (RSM). Bioresour Technol 99 (2008) 2025-2031
43. Zhang J., Liu X., Xu Z., Chen H., and Yang Y. Degradation of chlorophenols catalyzed by laccase. Int Biodeter Biodegr 61 (2008) 351-356
44. Aktaş N., and Tanyolaç A. Reaction conditions for laccase catalyzed polymerization of catechol. Bioresour Technol 87 3 (2003) 209-214
45. Kunamneni A., Camarero S., García-Burgos C., Plou F.J., Ballesteros A., and Alcalde M. Engineering and applications of fungal laccases for organic synthesis. Microb Cell Factories 7 (2008) 32 10.1186/1475-2859-7-32