Integrating cell-free biosyntheses of heme prosthetic group and apoenzyme for the synthesis of functional P450 monooxygenase

Biotechnology and Bioengineering

Volumn 110, Issue 4, 2013, Pages 1193-1200

  Kwon, Yong Chan ^a   Oh, In Seok ^b   Lee, Nahum ^b   Lee, Kyung Ho ^a   Yoon, Yeo Joon ^c   Lee, Eun Yeol ^d   Kim, Byung Gee ^b   Kim, Dong Myung ^a  

^a Chungnam National University   (South Korea)

^b Seoul National University   (South Korea)

^c Ewha Womans University   (South Korea)

^d Kyung Hee University   (South Korea)

Author keywords

Cell free protein synthesis; Cytochrome P450 monooxygenase; Heme; In vitro metabolic engineering; Prosthetic group

Indexed keywords

CELL FREE PROTEIN SYNTHESIS; HEME; IN-VITRO; MONOOXYGENASES; PROSTHETIC GROUPS;

BIOACTIVITY; BIOSYNTHESIS; ENZYMES; GENES; MACHINERY; PORPHYRINS; PROTEINS;

PROSTHETICS;

5 AMINOLEVULINATE SYNTHASE; ADENOSINE TRIPHOSPHATE; APOENZYME; CREATINE KINASE; CYTOCHROME P450; GLUTAMATE DEHYDROGENASE; HEME; P450 BM3M2 APOENZYME; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE;

ARTICLE; BINDING AFFINITY; BIOGENESIS; CELL FREE SYSTEM; CELL TRANSFORMATION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; EUKARYOTIC CELL; EXPRESSION VECTOR; FLUORESCENCE; HEME SYNTHESIS; HYDROXYLATION; IN VITRO STUDY; METABOLIC ENGINEERING; NONHUMAN; OPEN READING FRAME; PLASMID; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; RESTRICTION SITE;

CELL-FREE SYSTEM; CYTOCHROME P-450 ENZYME SYSTEM; HEME;

EID: 84874201521     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24785     Document Type: Article

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