Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis

Biotechnology Journal

Volumn 9, Issue 5, 2014, Pages 630-640

  Schoborg, Jennifer A ^a,b   Hodgman, C Eric ^a,b   Anderson, Mark J ^a,b   Jewett, Michael C ^a,b,c  

^a Northwestern University   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Cell free biology; Cell free protein synthesis; In vitro translation; Protein expression; Saccharomyces cerevisiae

Indexed keywords

AMINO ACIDS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; PROTEINS; SUBSTRATES; YEAST;

ACID CONCENTRATIONS; CELL FREE PROTEIN SYNTHESIS; CELL-FREE BIOLOGIES; GREEN FLUORESCENT PROTEIN; IN-VITRO; INORGANIC PHOSPHATES; NUCLEOSIDE TRIPHOSPHATES; PROTEIN EXPRESSIONS;

BIOSYNTHESIS;

AMINO ACID; ASPARTIC ACID; CREATINE PHOSPHATE; GREEN FLUORESCENT PROTEIN; NUCLEOSIDE TRIPHOSPHATE; PHOSPHATE; CREATINE; NUCLEOTIDE; RECOMBINANT PROTEIN;

ARTICLE; CONCENTRATION (PARAMETERS); DIFFUSION; ENERGY; FED BATCH CULTURE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SACCHAROMYCES CEREVISIAE; YEAST CELL; BIOTECHNOLOGY; CELL FREE SYSTEM; CHEMISTRY; CULTURE TECHNIQUE; DEVICES; EQUIPMENT DESIGN; METABOLISM; MICROBIOLOGY; PROCEDURES;

SACCHAROMYCES CEREVISIAE;

AMINO ACIDS; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CELL-FREE SYSTEM; CREATINE; EQUIPMENT DESIGN; NUCLEOTIDES; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE;

EID: 84899922092     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201300383     Document Type: Article

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