Synthesis and site-directed fluorescence labeling of azido proteins using eukaryotic cell-free orthogonal translation systems

Analytical Biochemistry

Volumn 451, Issue 1, 2014, Pages 4-9

  Quast, Robert B ^a   Claussnitzer, Iris ^b   Merk, Helmut ^b   Kubick, Stefan ^a   Gerrits, Michael ^b  

^a FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^b RiNA GmbH   (Germany)

Author keywords

Cell free protein synthesis; Eukaryotic; Microsomal vesicles; Site directed fluorescence labeling; Staudinger ligation; Translocation

Indexed keywords

AMBER; AMINO ACIDS; CELLS; CYTOLOGY; FLUORESCENCE; PROTEINS;

CELL FREE PROTEIN SYNTHESIS; EUKARYOTIC; FLUORESCENCE LABELING; MICROSOMAL VESICLES; STAUDINGER LIGATION; TRANSLOCATION;

BIOSYNTHESIS;

AMINO ACID TRANSFER RNA LIGASE; AZIDE; BETA GLUCURONIDASE; ERYTHROPOIETIN; FLUORESCENT DYE; PHENYLALANINE; SIGNAL PEPTIDE; SYNTHETASE; TRANSFER RNA;

ARTICLE; CELL FREE SYSTEM; CONTROLLED STUDY; EUKARYOTIC CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SYNTHESIS; SPODOPTERA FRUGIPERDA; WHEAT GERM;

CELL-FREE PROTEIN SYNTHESIS; EUKARYOTIC; MICROSOMAL VESICLES; SITE-DIRECTED FLUORESCENCE LABELING; STAUDINGER LIGATION; TRANSLOCATION;

AMINO ACYL-TRNA SYNTHETASES; ANIMALS; AZIDES; CELL-FREE SYSTEM; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ERYTHROPOIETIN; FLUORESCENT DYES; GLUCURONIDASE; HUMANS; PHENYLALANINE; SF9 CELLS; TRITICUM;

EID: 84896826917     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2014.01.013     Document Type: Article

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