Use of transposase and ends of IS608 enables precise and scarless genome modification for modulating gene expression and metabolic engineering applications in Escherichia coli

Biotechnology Journal

Volumn 11, Issue 1, 2016, Pages 80-90

  Thakker, Chandresh ^a,b   Lin, Kevin ^a   Martini Stoica, Heidi ^a,c   Bennett, George N ^a  

^a RICE UNIVERSITY   (United States)

^b Total Gas and Power New Energies USA Inc   (United States)

^c BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

Counterselection; Genome modification; IS608; Red recombinase; Transposase

Indexed keywords

ANTIBIOTICS; BACTERIA; CHROMOSOMES; ESCHERICHIA COLI; GENE EXPRESSION; METABOLIC ENGINEERING; POLYMERASE CHAIN REACTION;

COUNTERSELECTION; GENOME MODIFICATION; IS608; RED RECOMBINASE; TRANSPOSASE;

GENES;

BETA GALACTOSIDASE; DNA; TRANSPOSASE; TRANSPOSON;

ANTIBIOTIC RESISTANCE; ARTICLE; BINDING SITE; CHROMOSOME; ESCHERICHIA COLI; GENE; GENE DISRUPTION; GENE EXPRESSION; GENE LIBRARY; GENE SEQUENCE; IS608 GENE; METABOLIC ENGINEERING; NONHUMAN; PLASMID; POINT MUTATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RIBOSOME; GENETIC RECOMBINATION; GENETICS; LACTOSE OPERON; METABOLISM; TRANSPOSON;

BETA-GALACTOSIDASE; DNA TRANSPOSABLE ELEMENTS; ESCHERICHIA COLI; GENE EXPRESSION; LAC OPERON; METABOLIC ENGINEERING; POINT MUTATION; RECOMBINATION, GENETIC; TRANSPOSASES;

EID: 84955191442     PISSN: 18606768     EISSN: 18607314     Source Type: Journal    
DOI: 10.1002/biot.201500205     Document Type: Article

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