Sinorhizobium meliloti dctA mutants with partial ability to transport dicarboxylic acids

Journal of Bacteriology

Volumn 187, Issue 3, 2005, Pages 1161-1172

  Yurgel, Svetlana N ^a   Kahn, Michael L ^a  

^a WASHINGTON STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARRIER PROTEIN; DICARBOXYLIC ACID; FLUOROOROTIC ACID; FUMARIC ACID; GLUTAMATE TRANSPORTER; GLYCINE; MALIC ACID; MEMBRANE PROTEIN; NITROGEN; SUCCINIC ACID;

ALFALFA; AMINO ACID TRANSPORT; ARTICLE; BACTERIUM ISOLATION; BACTERIUM MUTANT; CELL GROWTH; DELETION MUTANT; NITROGEN FIXATION; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN MOTIF; SINORHIZOBIUM MELILOTI; SYMBIOSIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; BIOLOGICAL TRANSPORT; DICARBOXYLIC ACID TRANSPORTERS; DICARBOXYLIC ACIDS; DNA PRIMERS; MEDICAGO SATIVA; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; NITROGEN FIXATION; PLANT ROOTS; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SEQUENCE DELETION; SEQUENCE HOMOLOGY, AMINO ACID; SINORHIZOBIUM MELILOTI; SYMBIOSIS;

BACTERIA (MICROORGANISMS); MEDICAGO SATIVA; NEGIBACTERIA; SINORHIZOBIUM MELILOTI;

EID: 13244295377     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.187.3.1161-1172.2005     Document Type: Article

References (31)

1. Baker, K. E., K. P. Ditullio, J. Neuhard, and R. A. Kelln. 1996. Utilization of orotate as a pyrimidine source by Salmonella typhimurium and Escherichia coli required the dicarboxylate transport protein encoded by dctA. J. Bacteriol. 178:7099-7105.
2. Bendahan, A., A. Armon, N. Madani, M. P. Kavanaugh, and B. I. Kanner. 2000. Arginine 447 plays a pivotal role in substrate interactions in a neuronal glutamate transporter. J. Biol. Chem. 275:37436-37442.
3. Charles, T. C., and T. M. Finan. 1991. Analysis of a 1,600-kilobase Rhizobium meliloti megaplasmid using defined deletions generated in vivo. Genetics 127:5-20.
4. Choi, I., and S. Y. Chiu. 2000. Loss of cell viability by histidine substitution of leucine 325 of the glutamate transporter EAAAT1. Biochem. Biophys. Res. Commun. 275:382-385.
5. Gardiol, A., A. Arias, C. Cervenansky, and G. Martinez-Drets. 1982. Succinate dehydrogenase mutant of Rhizobium meliloti. J. Bacteriol. 15:1621-1623.
6. Grzemski, W., J. P. Akowski, and M. L. Kahn. Altering bacterial metabolism in a nitrogen fixing symbiosis using conditional and impaired mutations in Sinorhizobium meliloti citrate synthase. Mol. Plant Microbiol. Int., in press.
7. Humphreys, G. O., G. A. Willshaw, H. R. Smith, and E. S. Anderson. 1976. Mutagenesis of plasmid DNA with hydroxylamine: isolation of mutants of multi-copy plasmids. Mol. Gen. Genet. 145:101-108.
8. 4-dicarboxylate permease (DctA) as derived from protein fusions with Escherichia coli K12 alkaline phosphatase (PhoA) and β-galactosidase (LacZ). Mol. Gen. Genet. 241:106-114.
9. Kahn, M. L., T. R. McDermott, and M. K. Udvardi. 1998. Carbon and nitrogen metabolism in rhizobia, p. 461-485. In H. P. Spaink, A. Kondorosi, and P. J. J. Hooykaas (ed.), The Rhizobiaceae. Kluwer Academic Publisher, Dordrecht, The Netherlands.
10. Kavanaugh, M. P., A. Bendahan, N. Zerangue, Y. Zhang, and B. I. Kanner. 1997. Mutation of an amino acid residue influencing potassium coupling in the glutamate transporter GLT-1 induces obligate exchange. J. Biol. Chem. 272:1703-1708.
11. Leigh, J. A., E. R. Signer, and G. C. Walker. 1985. Exopolysaccharide- deficient mutants of Rhizobium meliloti that form ineffective nodules. Proc. Natl. Acad. Sci. USA 82:6231-6235.
12. Lolkema, J. S., and D. J. Slotboom. 1998. Estimation of structural similarity of membrane proteins by hydropathy profile alignment. Mol. Membr. Biol. 15:33-42.
13. McDermott, T. R., and M. L. Kahn. 1992. Cloning and mutagenesis of the Rhizobium meliloti isocitrate dehydrogenase gene. J. Bacteriol. 174:4790-4797.
14. Mitrovie, A. D., S. G. Amara, G. A. Johnston, and R. J. Vandenberg. 1998. Identification of functional domains of the human glutamate transporters EAAT1 and EAAT2. J. Biol. Chem. 273:14698-14706.
15. Pines, G., N. C. Danbolt, M. Bjoras, Y. Zhang, A. Bendahan, L. Eide, H. Koepsell, J. Storm-Mathisen, E. Seeberg, and B. I. Kanner. 1992. Cloning and expression of a rat brain L-glutamate transporter. Nature 360:464-467.
16. +)-coupled glutamate transporter form rat brain. J. Biol. Chem. 270:17093-17097.
17. Reid, C. J., and P. S. Poole. 1998. Roles of DctA and DctB in signal detection by the dicarboxylic acid transport system of Rhizobium leguminosarum. J. Bacteriol. 180:2660-2669.
18. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Schafer, A., A. Tauch, W. Jager, J. Kalinowski, G. Thierbach, and A. Puhler. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73.
20. Simon, R., U. Priefer, and A. Pühler. 1983. Vector plasmids for in-vivo and in-vitro manipulations of gram-negative bacteria, p. 98-106. In A. Pühler (ed.), Molecular genetics of the bacterial-plant interaction: the Rhizobium meliloti-Medicago sativa system. Springer-Verlag, Berlin, Germany.
21. Slotboom, D. G., J. S. Lolkema, and W. N. Konings. 1996. Membrane topology of the C-terminal half of the neuronal, glial, and bacterial glutamate transporter family. J. Biol. Chem. 271:31317-31321.
22. Slotboom, D. G., I. Sobczak, W. N. Konings, and J. S. Lolkema. 1999. A conserved serine-rich stretch in the glutamate transporter family forms a substrate-sensitive loop. Proc. Natl. Acad. Sci. USA 96:1482-1487.
23. Somerville, J. E., and M. L. Kahn. 1983. Cloning of the glutamine synthetase I gene from Rhizobium meliloti. J. Bacteriol. 156:168-176.
24. Vasse, J., F. DeBilly, S. Camut, and G. Truchet. 1990. Correlation between ultrastructural differentiation of bacteroids and nitrogen fixation in alfalfa nodules. J. Bacteriol. 172:4295-4306.
25. 4-dicarboxylate transport and symbiotic nitrogen fixation are located on a megaplasmid. J. Bacteriol. 170:927-934.
26. 4-dicarboxylate transport genes in Rhizobium meliloti. Mol. Microbiol. 3:813-823.
27. Yurgel, S., M. W. Mortimer, K. N. Rogers., and M. L. Kahn. 2000. New substrates for the dicarboxylate transport system of Sinorhizobium meliloti. J. Bacteriol. 182:4216-4221.
28. Yurgel, S. N., and M. L. Kahn. 2000. Unpublished observations.
29. Zarbiv, R., M. Grunewald, M. P. Kavanaugh, and B. I. Kanner. 1998. Cysteine scanning of the surroundings of an alkali-ion binding site of the glutamate transporter GLT-1 reveals a conformationally sensitive residue. J. Biol. Chem. 273:14231-14237.
30. +)-coupled rat brain glutamate transporter. Proc. Natl. Acad. Sci. USA 95:751-755.
31. Ziegler, M. A. 2004. Personal communication.